BRENDA - Enzyme Database

The purification and properties of monoacylglycerol kinase from bovine brain

Shim, Y.H.; Lin, C.H.; Strickland, K.P.; Biochem. Cell Biol. 67, 233-241 (1989)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
2.7.1.94
additional information
protein peak eluted from hydroxylapatite by 25 mM phosphate activates and stabilizes
Bos taurus
2.7.1.94
Phospholipids
e.g. phosphatidylcholine, phosphatidylethanolamine or sphingomyelin, inhibition alone, synergistic activation with the protein peak eluted from hydroxylapatite by 25 mM phosphate
Bos taurus
General Stability
EC Number
General Stability
Organism
2.7.1.94
DTT, EDTA or ATP stabilizes during purification
Bos taurus
2.7.1.94
protein peak eluted from hydroxylapatite by 25 mM phosphate activates and stabilizes
Bos taurus
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
2.7.1.94
5,5'-dithiobis(2-nitrobenzoic acid)
DTT prevents inhibition
Bos taurus
2.7.1.94
NEM
DTT prevents inhibition
Bos taurus
2.7.1.94
p-chloromercuriphenylsulfonate
DTT prevents inhibition
Bos taurus
2.7.1.94
phosphatidylcholine
inhibition alone, synergistic activation with the protein peak eluted from hydroxylapatite by 25 mM phosphate
Bos taurus
2.7.1.94
phosphatidylethanolamine
inhibition alone, synergistic activation with the protein peak eluted from hydroxylapatite by 25 mM phosphate
Bos taurus
2.7.1.94
sphingomyelin
inhibition alone, synergistic activation with the protein peak eluted from hydroxylapatite by 25 mM phosphate
Bos taurus
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.7.1.94
cytosol
-
Bos taurus
5829
-
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
2.7.1.94
Ca2+
5 mM, 58% as effective as Mg2+ in activation
Bos taurus
2.7.1.94
Co2+
5 mM, 60% as effective as Mg2+ in activation
Bos taurus
2.7.1.94
Cu2+
5 mM, 51% as effective as Mg2+ in activation
Bos taurus
2.7.1.94
Fe2+
5 mM, 60% as effective as Mg2+ in activation
Bos taurus
2.7.1.94
Mg2+
optimal concentration is 10 mM; required
Bos taurus
2.7.1.94
Mn2+
5 mM, as effective as Mg2+ in activation
Bos taurus
2.7.1.94
Ni2+
5 mM, 60% as effective as Mg2+ in activation
Bos taurus
2.7.1.94
Zn2+
5 mM, 48% as effective as Mg2+ in activation
Bos taurus
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.7.1.94
72000
-
x * 72000, SDS-PAGE, enzyme exists as a complex that is stabilized by 0.5 M NaCl and, on complete dissociation yields a major protein of MW 72000
Bos taurus
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.7.1.94
Bos taurus
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
2.7.1.94
-
Bos taurus
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
2.7.1.94
brain
-
Bos taurus
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.7.1.94
additional information
-
-
Bos taurus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.1.94
ATP + 1-arachidonoylglycerol
-
642127
Bos taurus
ADP + 1-arachidonoylglycerol 3-phosphate
-
-
-
?
2.7.1.94
ATP + 1-arachidoylglycerol
-
642127
Bos taurus
ADP + 1-arachidoylglycerol 3-phosphate
-
-
-
?
2.7.1.94
ATP + 1-caproylglycerol
-
642127
Bos taurus
ADP + 1-caproylglycerol 3-phosphate
-
-
-
?
2.7.1.94
ATP + 1-capryloylglycerol
-
642127
Bos taurus
ADP + 1-capryloylglycerol 3-phosphate
-
-
-
?
2.7.1.94
ATP + 1-lauroylglycerol
-
642127
Bos taurus
ADP + 1-lauroylglycerol 3-phosphate
-
-
-
?
2.7.1.94
ATP + 1-linolenoylglycerol
-
642127
Bos taurus
ADP + 1-linolenoylglycerol 3-phosphate
-
-
-
?
2.7.1.94
ATP + 1-linoleoylglycerol
-
642127
Bos taurus
ADP + 1-linoleoylglycerol 3-phosphate
-
-
-
?
2.7.1.94
ATP + 1-monoolein
i.e. 1-monooleolylglyerol
642127
Bos taurus
ADP + 1-oleoylglycerol 3-phosphate
-
-
-
?
2.7.1.94
ATP + 1-myristoylglycerol
-
642127
Bos taurus
ADP + 1-myristoylglycerol 3-phosphate
-
-
-
?
2.7.1.94
ATP + 1-palmitoleoylglycerol
-
642127
Bos taurus
ADP + 1-palmitoleoylglycerol 3-phosphate
-
-
-
?
2.7.1.94
ATP + 1-palmitoylglycerol
-
642127
Bos taurus
ADP + 1-palmitoylglycerol 3-phosphate
-
-
-
?
2.7.1.94
ATP + 1-stearoylglycerol
-
642127
Bos taurus
ADP + 1-stearoylglycerol 3-phosphate
-
-
-
?
2.7.1.94
ATP + 2-arachidonoylglycerol
-
642127
Bos taurus
ADP + 2-arachidonoylglycerol 3-phosphate
-
-
-
?
2.7.1.94
ATP + 2-arachidoylglycerol
-
642127
Bos taurus
ADP + 2-arachidoylglycerol 3-phosphate
-
-
-
?
2.7.1.94
ATP + 2-caproylglycerol
-
642127
Bos taurus
ADP + 2-caproylglycerol 3-phosphate
-
-
-
?
2.7.1.94
ATP + 2-lauroylglycerol
-
642127
Bos taurus
ADP + 2-lauroylglycerol 3-phosphate
-
-
-
?
2.7.1.94
ATP + 2-linolenoylglycerol
-
642127
Bos taurus
ADP + 2-linolenoylglycerol 3-phosphate
-
-
-
?
2.7.1.94
ATP + 2-linoleoylglycerol
-
642127
Bos taurus
ADP + 2-linoleoylglycerol 3-phosphate
-
-
-
?
2.7.1.94
ATP + 2-monoolein
i.e. 2-monooleolylglyerol
642127
Bos taurus
ADP + 2-oleoyoglycerol 3-phosphate
-
-
-
?
2.7.1.94
ATP + 2-palmitoleoylglycerol
-
642127
Bos taurus
ADP + 2-palmitoleoylglycerol 3-phosphate
-
-
-
?
2.7.1.94
ATP + 2-palmitoylglycerol
-
642127
Bos taurus
ADP + 2-palmitoylglycerol 3-phosphate
-
-
-
?
2.7.1.94
ATP + 2-stearoylglycerol
-
642127
Bos taurus
ADP + 2-stearoylglycerol 3-phosphate
-
-
-
?
2.7.1.94
additional information
1-monostearin amongst the 1-acylglycerols and 2-monoarachidonin amongst the 2-acylglycerols give the highest activity
642127
Bos taurus
?
-
-
-
-
2.7.1.94
additional information
preference for substrates with unsaturated fatty acids except for 1- and 2-monostearins
642127
Bos taurus
?
-
-
-
-
Subunits
EC Number
Subunits
Commentary
Organism
2.7.1.94
?
x * 72000, SDS-PAGE, enzyme exists as a complex that is stabilized by 0.5 M NaCl and, on complete dissociation yields a major protein of MW 72000
Bos taurus
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
2.7.1.94
additional information
protein peak eluted from hydroxylapatite by 25 mM phosphate activates and stabilizes
Bos taurus
2.7.1.94
Phospholipids
e.g. phosphatidylcholine, phosphatidylethanolamine or sphingomyelin, inhibition alone, synergistic activation with the protein peak eluted from hydroxylapatite by 25 mM phosphate
Bos taurus
General Stability (protein specific)
EC Number
General Stability
Organism
2.7.1.94
DTT, EDTA or ATP stabilizes during purification
Bos taurus
2.7.1.94
protein peak eluted from hydroxylapatite by 25 mM phosphate activates and stabilizes
Bos taurus
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
2.7.1.94
5,5'-dithiobis(2-nitrobenzoic acid)
DTT prevents inhibition
Bos taurus
2.7.1.94
NEM
DTT prevents inhibition
Bos taurus
2.7.1.94
p-chloromercuriphenylsulfonate
DTT prevents inhibition
Bos taurus
2.7.1.94
phosphatidylcholine
inhibition alone, synergistic activation with the protein peak eluted from hydroxylapatite by 25 mM phosphate
Bos taurus
2.7.1.94
phosphatidylethanolamine
inhibition alone, synergistic activation with the protein peak eluted from hydroxylapatite by 25 mM phosphate
Bos taurus
2.7.1.94
sphingomyelin
inhibition alone, synergistic activation with the protein peak eluted from hydroxylapatite by 25 mM phosphate
Bos taurus
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.7.1.94
cytosol
-
Bos taurus
5829
-
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
2.7.1.94
Ca2+
5 mM, 58% as effective as Mg2+ in activation
Bos taurus
2.7.1.94
Co2+
5 mM, 60% as effective as Mg2+ in activation
Bos taurus
2.7.1.94
Cu2+
5 mM, 51% as effective as Mg2+ in activation
Bos taurus
2.7.1.94
Fe2+
5 mM, 60% as effective as Mg2+ in activation
Bos taurus
2.7.1.94
Mg2+
optimal concentration is 10 mM; required
Bos taurus
2.7.1.94
Mn2+
5 mM, as effective as Mg2+ in activation
Bos taurus
2.7.1.94
Ni2+
5 mM, 60% as effective as Mg2+ in activation
Bos taurus
2.7.1.94
Zn2+
5 mM, 48% as effective as Mg2+ in activation
Bos taurus
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.7.1.94
72000
-
x * 72000, SDS-PAGE, enzyme exists as a complex that is stabilized by 0.5 M NaCl and, on complete dissociation yields a major protein of MW 72000
Bos taurus
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.7.1.94
-
Bos taurus
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
2.7.1.94
brain
-
Bos taurus
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.7.1.94
additional information
-
-
Bos taurus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.1.94
ATP + 1-arachidonoylglycerol
-
642127
Bos taurus
ADP + 1-arachidonoylglycerol 3-phosphate
-
-
-
?
2.7.1.94
ATP + 1-arachidoylglycerol
-
642127
Bos taurus
ADP + 1-arachidoylglycerol 3-phosphate
-
-
-
?
2.7.1.94
ATP + 1-caproylglycerol
-
642127
Bos taurus
ADP + 1-caproylglycerol 3-phosphate
-
-
-
?
2.7.1.94
ATP + 1-capryloylglycerol
-
642127
Bos taurus
ADP + 1-capryloylglycerol 3-phosphate
-
-
-
?
2.7.1.94
ATP + 1-lauroylglycerol
-
642127
Bos taurus
ADP + 1-lauroylglycerol 3-phosphate
-
-
-
?
2.7.1.94
ATP + 1-linolenoylglycerol
-
642127
Bos taurus
ADP + 1-linolenoylglycerol 3-phosphate
-
-
-
?
2.7.1.94
ATP + 1-linoleoylglycerol
-
642127
Bos taurus
ADP + 1-linoleoylglycerol 3-phosphate
-
-
-
?
2.7.1.94
ATP + 1-monoolein
i.e. 1-monooleolylglyerol
642127
Bos taurus
ADP + 1-oleoylglycerol 3-phosphate
-
-
-
?
2.7.1.94
ATP + 1-myristoylglycerol
-
642127
Bos taurus
ADP + 1-myristoylglycerol 3-phosphate
-
-
-
?
2.7.1.94
ATP + 1-palmitoleoylglycerol
-
642127
Bos taurus
ADP + 1-palmitoleoylglycerol 3-phosphate
-
-
-
?
2.7.1.94
ATP + 1-palmitoylglycerol
-
642127
Bos taurus
ADP + 1-palmitoylglycerol 3-phosphate
-
-
-
?
2.7.1.94
ATP + 1-stearoylglycerol
-
642127
Bos taurus
ADP + 1-stearoylglycerol 3-phosphate
-
-
-
?
2.7.1.94
ATP + 2-arachidonoylglycerol
-
642127
Bos taurus
ADP + 2-arachidonoylglycerol 3-phosphate
-
-
-
?
2.7.1.94
ATP + 2-arachidoylglycerol
-
642127
Bos taurus
ADP + 2-arachidoylglycerol 3-phosphate
-
-
-
?
2.7.1.94
ATP + 2-caproylglycerol
-
642127
Bos taurus
ADP + 2-caproylglycerol 3-phosphate
-
-
-
?
2.7.1.94
ATP + 2-lauroylglycerol
-
642127
Bos taurus
ADP + 2-lauroylglycerol 3-phosphate
-
-
-
?
2.7.1.94
ATP + 2-linolenoylglycerol
-
642127
Bos taurus
ADP + 2-linolenoylglycerol 3-phosphate
-
-
-
?
2.7.1.94
ATP + 2-linoleoylglycerol
-
642127
Bos taurus
ADP + 2-linoleoylglycerol 3-phosphate
-
-
-
?
2.7.1.94
ATP + 2-monoolein
i.e. 2-monooleolylglyerol
642127
Bos taurus
ADP + 2-oleoyoglycerol 3-phosphate
-
-
-
?
2.7.1.94
ATP + 2-palmitoleoylglycerol
-
642127
Bos taurus
ADP + 2-palmitoleoylglycerol 3-phosphate
-
-
-
?
2.7.1.94
ATP + 2-palmitoylglycerol
-
642127
Bos taurus
ADP + 2-palmitoylglycerol 3-phosphate
-
-
-
?
2.7.1.94
ATP + 2-stearoylglycerol
-
642127
Bos taurus
ADP + 2-stearoylglycerol 3-phosphate
-
-
-
?
2.7.1.94
additional information
1-monostearin amongst the 1-acylglycerols and 2-monoarachidonin amongst the 2-acylglycerols give the highest activity
642127
Bos taurus
?
-
-
-
-
2.7.1.94
additional information
preference for substrates with unsaturated fatty acids except for 1- and 2-monostearins
642127
Bos taurus
?
-
-
-
-
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
2.7.1.94
?
x * 72000, SDS-PAGE, enzyme exists as a complex that is stabilized by 0.5 M NaCl and, on complete dissociation yields a major protein of MW 72000
Bos taurus