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Literature summary extracted from

  • Kobayashi, M.; Funane, K.; Ohya, S.; Torikoshi, H.; Kurogi, M.; Ishiuchi, D.
    Pyrophosphate-dependent phosphofructokinase: its oligomeric froms in mature green tomato fruit (1992), Biosci. Biotechnol. Biochem., 56, 54-57.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.1.90 D-fructose 2,6-bisphosphate 11fold Solanum lycopersicum
2.7.1.90 D-fructose 2,6-bisphosphate forward reaction only Solanum lycopersicum
2.7.1.90 D-glucose forward reaction Solanum lycopersicum
2.7.1.90 D-glucose 1-phosphate reverse reaction Solanum lycopersicum

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.90 Ca2+ complete inhibition in the forward reaction, slightly inhibitory in the reverse reaction Solanum lycopersicum
2.7.1.90 Cl-
-
 Solanum lycopersicum
2.7.1.90 Co2+ complete inhibition in the forward reaction, strong inhibition in the reverse reaction Solanum lycopersicum
2.7.1.90 diphosphate
-
 Solanum lycopersicum
2.7.1.90 EDTA
-
 Solanum lycopersicum
2.7.1.90 KCl complete inhibition in the forward reaction, slightly inhibitory in the reverse reaction Solanum lycopersicum
2.7.1.90 Mn2+ inhibitory in the forward reaction only, slightly activating in the reverse reaction Solanum lycopersicum
2.7.1.90 NaCl complete inhibition in the forward reaction, slightly inhibitory in the reverse reaction Solanum lycopersicum
2.7.1.90 NH4+ complete inhibition in the forward reaction, slightly inhibitory in the reverse reaction Solanum lycopersicum
2.7.1.90 SO42- gluconeogentic reaction Solanum lycopersicum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.90 0.3 0.38 D-fructose 6-phosphate
-
 Solanum lycopersicum

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.90 Co2+ no activation of forward reaction Solanum lycopersicum
2.7.1.90 Co2+ no activitaion Solanum lycopersicum
2.7.1.90 K+ slightly activating in forward reaction Solanum lycopersicum
2.7.1.90 Mg2+ requirement Solanum lycopersicum
2.7.1.90 Mg2+ less effective than Mn2+ Solanum lycopersicum
2.7.1.90 Mn2+ inhibitory in the forward reaction only, slightly activating in the reverse reaction Solanum lycopersicum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.90 61000 62000 PAGE, gel filtration Solanum lycopersicum
2.7.1.90 64000
-
 x * 64000 + x * 68000, SDS-PAGE Solanum lycopersicum
2.7.1.90 68000
-
 x * 64000 + x * 68000, SDS-PAGE Solanum lycopersicum
2.7.1.90 123000 136000 dimer, PAGE, gel filtration Solanum lycopersicum
2.7.1.90 243000 262000 predominant tetrameric form, PAGE, gel filtration, in the presence of diphosphate the oligomer dissociates into monomers, D-fructose 2,6-bisphosphate does not reassociate Solanum lycopersicum
2.7.1.90 425000
-
 hexamer, PAGE, in the presence of diphosphate the oligomer dissociates into monomers, D-fructose 2,6-bisphosphate does not reassociate Solanum lycopersicum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.90 diphosphate + D-fructose 6-phosphate Solanum lycopersicum
-
 phosphate + D-fructose 1,6-bisphosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.90 Solanum lycopersicum
-
 cv. Super First
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.90 partial, 569fold Solanum lycopersicum

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.1.90 fruit mature green Solanum lycopersicum
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.90 1.72
-
 partially purified enzyme Solanum lycopersicum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.90 diphosphate + D-fructose 6-phosphate
-
 Solanum lycopersicum phosphate + D-fructose 1,6-bisphosphate
-
 ?
2.7.1.90 diphosphate + D-fructose 6-phosphate
-
 Solanum lycopersicum phosphate + D-fructose 1,6-bisphosphate
-
 r

Subunits

EC Number Subunits Comment Organism
2.7.1.90 oligomer x * 64000 + x * 68000, SDS-PAGE Solanum lycopersicum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.1.90 25
-
 assay at Solanum lycopersicum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.90 8
-
 assay at Solanum lycopersicum

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.7.1.90 1.3
-
 diphosphate pH 8.0, 25°C Solanum lycopersicum