Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Palmer, R.E.; Anderson, R.L.
    Cellobiose metabolism in Aerobacter aerogenes. II. Phosphorylation of cellobiose with adenosine 5-triphosphate by alpha-glucoside kinase (1972), J. Biol. Chem., 247, 3415-3419.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.85 0.6
-
 arbutin pH 7.5 Klebsiella aerogenes
2.7.1.85 1
-
 cellobiose pH 7.5 Klebsiella aerogenes
2.7.1.85 1.5
-
 gentiobiose pH 7.5 Klebsiella aerogenes
2.7.1.85 1.7
-
 ATP pH 7.5 Klebsiella aerogenes
2.7.1.85 2.5
-
 Salicin pH 7.5 Klebsiella aerogenes
2.7.1.85 2.5
-
 phenyl beta-D-glucoside pH 7.5 Klebsiella aerogenes
2.7.1.85 3.1
-
 amygdalin pH 7.5 Klebsiella aerogenes
2.7.1.85 3.1
-
 sophorose pH 7.5 Klebsiella aerogenes
2.7.1.85 4
-
 cellobiitol pH 7.5 Klebsiella aerogenes
2.7.1.85 6.3
-
 cellotetraose pH 7.5 Klebsiella aerogenes
2.7.1.85 6.7
-
 cellotriose pH 7.5 Klebsiella aerogenes
2.7.1.85 13.1
-
 methyl beta-D-glucoside pH 7.5 Klebsiella aerogenes

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.85 150000
-
 sucrose density gradient centrifugation Klebsiella aerogenes

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.85 ATP + cellobiose Klebsiella aerogenes first reaction in metabolism of cellobiose, inducible enzyme ADP + 6-phospho-beta-D-glucosyl-(1,4)-D-glucose
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.85 Klebsiella aerogenes
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.85
-
 Klebsiella aerogenes

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.85 45
-
-
 Klebsiella aerogenes

Storage Stability

EC Number Storage Stability Organism
2.7.1.85 4°C, 35% loss of activity after 2 months Klebsiella aerogenes
2.7.1.85 frozen, 50% loss of activity after 2 weeks Klebsiella aerogenes

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.85 ATP + amygdalin 21% of the activity with cellobiose Klebsiella aerogenes ADP + amygdalin 6-phosphate
-
 ?
2.7.1.85 ATP + arbutin 36% of the activity with cellobiose Klebsiella aerogenes ADP + arbutin 6-phosphate
-
 ?
2.7.1.85 ATP + cellobiitol 25% of the activity with cellobiose Klebsiella aerogenes ?
-
 ?
2.7.1.85 ATP + cellobiose
-
 Klebsiella aerogenes ADP + 6-phospho-beta-D-glucosyl-(1,4)-D-glucose
-
 ?
2.7.1.85 ATP + cellobiose first reaction in metabolism of cellobiose, inducible enzyme Klebsiella aerogenes ADP + 6-phospho-beta-D-glucosyl-(1,4)-D-glucose
-
 ?
2.7.1.85 ATP + cellotetraose 33% of the activity with cellobiose Klebsiella aerogenes ADP + 6-phospho-beta-glucosyl-(1,4)-beta-D-glucosyl-(1,4)-beta-D-glucosyl-(1,4)-D-glucose
-
 ?
2.7.1.85 ATP + cellotriose 47% of the activity with cellobiose Klebsiella aerogenes ADP + 6-phospho-beta-D-glucosyl-(1,4)-beta-D-glucosyl-(1,4)-D-glucose
-
 ?
2.7.1.85 ATP + gentiobiose 70% of the activity with cellobiose Klebsiella aerogenes ADP + 6-phospho-beta-D-glucosyl-(1,6)-D-glucose
-
 ?
2.7.1.85 ATP + methyl beta-D-glucoside as active as cellobiose Klebsiella aerogenes ADP + methyl beta-D-glucoside 6-phosphate
-
 ?
2.7.1.85 ATP + phenyl beta-D-glucoside 70% of the activity with cellobiose Klebsiella aerogenes ADP + phenyl beta-D-glucoside 6-phosphate
-
 ?
2.7.1.85 ATP + salicin 87% of the activity with cellobiose Klebsiella aerogenes ADP + salicin 6-phosphate
-
 ?
2.7.1.85 ATP + sophorose 19% of the activity with cellobiose Klebsiella aerogenes ADP + 6-phospho-beta-D-glucopyranosyl-(1,2)-D-glucose
-
 ?
2.7.1.85 CTP + cellobiose 12% of the activity with ATP Klebsiella aerogenes CDP + 6-phospho-beta-D-glucosyl-(1,4)-D-glucose
-
 ?
2.7.1.85 GTP + cellobiose 15% of the activity with ATP Klebsiella aerogenes GDP + 6-phospho-beta-D-glucosyl-(1,4)-D-glucose
-
 ?
2.7.1.85 ITP + cellobiose 7% of the activity with ATP Klebsiella aerogenes IDP + 6-phospho-beta-D-glucosyl-(1,4)-D-glucose
-
 ?
2.7.1.85 additional information not: ADP, acetyl phosphate, alpha-glycerophosphate, D-fructose, 1,6-diphosphate, phosphoramidate, choline phosphate, creatine phosphate, 3-phosphoglycerate, phenylphosphate, phosphoenolpyruvate Klebsiella aerogenes ?
-
 ?
2.7.1.85 UTP + cellobiose 3% of the activity with ATP Klebsiella aerogenes UDP + 6-phospho-beta-D-glucosyl-(1,4)-D-glucose
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.7.1.85 45
-
 half-life: 10 min Klebsiella aerogenes

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.85 7.3
-
 MES buffer Klebsiella aerogenes

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.7.1.85 6.2 8.2 pH 6.2: about 60% of maximal activity in Mes buffer, pH 8.2: reaction in PIPES buffer, about 70% of maximal activity in MES buffer Klebsiella aerogenes