Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Austin, G.E.; Sirakoff, D.; Roop, B.; Moyer, G.H.
    Purification and properties of polynucleotide kinase of calf thymus (1978), Biochim. Biophys. Acta, 522, 412-422.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.78 AgNO3 complete inhibition at 0.3 mM Bos taurus
2.7.1.78 ammonium sulfate 80% inhibition at 30 mM Bos taurus
2.7.1.78 CTP more than 90% inhibition at 0.3 mM Bos taurus
2.7.1.78 dATP more than 95% inhibition at 0.3 mM Bos taurus
2.7.1.78 dCTP 80% inhibition at 0.3 mM Bos taurus
2.7.1.78 Dextran sulfate
-
 Bos taurus
2.7.1.78 dGTP more than 95% inhibition at 0.3 mM Bos taurus
2.7.1.78 diphosphate 97% inhibition at 20 mM Bos taurus
2.7.1.78 dTTP more than 95% inhibition at 0.3 mM Bos taurus
2.7.1.78 GTP more than 90% inhibition at 0.3 mM Bos taurus
2.7.1.78 heparin
-
 Bos taurus
2.7.1.78 iodoacetate complete inhibition at 0.3 mM Bos taurus
2.7.1.78 additional information no inhibition by agar-agar Bos taurus
2.7.1.78 sulfate
-
 Bos taurus
2.7.1.78 sulfhydryl antagonists
-
 Bos taurus
2.7.1.78 UTP more than 90% inhibition at 0.3 mM Bos taurus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.78 0.004
-
 ATP pH 5.5, 37°C Bos taurus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.78 divalent cations absolutely required Bos taurus
2.7.1.78 Mg2+ Mg2+ or Mn2+ effective in activation Bos taurus
2.7.1.78 Mn2+ optimal concentration: 10 mM Bos taurus
2.7.1.78 Mn2+ Mg2+ or Mn2+ effective in activation Bos taurus
2.7.1.78 Zn2+ less effective Bos taurus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.78 70000
-
 gel filtration, sucrose density gradient centrifugation Bos taurus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.78 ATP + 5'-dephospho-DNA Bos taurus
-
 ADP + 5'-phospho-DNA
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.78 Bos taurus
-
 calf
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.78 1500fold Bos taurus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.1.78 thymus
-
 Bos taurus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.78 0.0043
-
 purified enzyme Bos taurus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.78 ATP + 5'-dephospho-DNA
-
 Bos taurus ADP + 5'-phospho-DNA
-
 ?
2.7.1.78 ATP + 5'-dephospho-DNA double stranded DNA Bos taurus ADP + 5'-phospho-DNA
-
 ?
2.7.1.78 ATP + 5'-dephospho-DNA single stranded DNA Bos taurus ADP + 5'-phospho-DNA
-
 ?
2.7.1.78 ATP + 5'-dephospho-RNA phosphorylated at a much lower rate than DNA Bos taurus ADP + 5'-phospho-RNA
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.1.78 monomer 1 * 70000-75000, SDS-PAGE Bos taurus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.1.78 37
-
 assay at Bos taurus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.78 5.5
-
-
 Bos taurus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.7.1.78 5 7 pH 5: about 5% of activity maximum, pH 7: about 30% of activity maximum Bos taurus

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.1.78 ATP dependent on Bos taurus