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Literature summary extracted from

  • Levin, C.J.; Zimmerman, S.B.
    A deoxyribonucleic acid kinase from nuclei of rat liver. Purification and properties (1976), J. Biol. Chem., 251, 1767-1774.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.1.78 bovine serum albumin
-
 Rattus norvegicus
2.7.1.78 spermidine little influence Rattus norvegicus
2.7.1.78 spermine little influence Rattus norvegicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.78 ADP
-
 Rattus norvegicus
2.7.1.78 AgNO3
-
 Rattus norvegicus
2.7.1.78 Cu2+ inhibits in presence of MgCl2 Rattus norvegicus
2.7.1.78 Deoxyribonucleoside triphosphates
-
 Rattus norvegicus
2.7.1.78 Dextran sulfate strong inhibition Rattus norvegicus
2.7.1.78 heparin strong inhibition Rattus norvegicus
2.7.1.78 iodoacetate
-
 Rattus norvegicus
2.7.1.78 p-chloromercuribenzoate
-
 Rattus norvegicus
2.7.1.78 Ribonucleoside triphosphates
-
 Rattus norvegicus
2.7.1.78 sulfate
-
 Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.78 0.002
-
 ATP pH 5.5, 37°C Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.1.78 nucleus
-
 Rattus norvegicus 5634
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.78 Ca2+ Mg2+, Mn2+, Co2+, Zn2+, Ni2+ or Ca2+ are effective Rattus norvegicus
2.7.1.78 Co2+ Mg2+, Mn2+, Co2+, Zn2+, Ni2+ or Ca2+ are effective Rattus norvegicus
2.7.1.78 divalent cations absolutely required Rattus norvegicus
2.7.1.78 divalent cations Mg2+, Mn2+, Co2+, Zn2+, Ni2+ or Ca2+ are effective, descending order Rattus norvegicus
2.7.1.78 Mg2+ Mg2+, Mn2+, Co2+, Zn2+, Ni2+ or Ca2+ are effective Rattus norvegicus
2.7.1.78 Mn2+ Mg2+, Mn2+, Co2+, Zn2+, Ni2+ or Ca2+ are effective Rattus norvegicus
2.7.1.78 Ni2+ Mg2+, Mn2+, Co2+, Zn2+, Ni2+ or Ca2+ are effective in activation Rattus norvegicus
2.7.1.78 Zn2+ Mg2+, Mn2+, Co2+, Zn2+, Ni2+ or Ca2+ are effective Rattus norvegicus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.78 80000
-
 gel filtration Rattus norvegicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.78 ATP + 5'-dephospho-DNA Rattus norvegicus
-
 ADP + 5'-phospho-DNA
-
 ?
2.7.1.78 ATP + 5'-dephospho-DNA Rattus norvegicus
-
 ADP + 5'-phospho-DNA
-
 r

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.78 Rattus norvegicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.78 partial, 960fold Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.1.78 liver
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.78 0.006
-
 partially purified enzyme Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.78 ATP + 5'-dephospho-DNA
-
 Rattus norvegicus ADP + 5'-phospho-DNA
-
 ?
2.7.1.78 ATP + 5'-dephospho-DNA
-
 Rattus norvegicus ADP + 5'-phospho-DNA
-
 r
2.7.1.78 ATP + 5'-dephospho-DNA double stranded DNA Rattus norvegicus ADP + 5'-phospho-DNA UDP, ADP, GDP and CDP support the reverse reaction r
2.7.1.78 ATP + 5'-dephospho-DNA large DNA fragments released by nuclease treatment Rattus norvegicus ADP + 5'-phospho-DNA UDP, ADP, GDP and CDP support the reverse reaction r
2.7.1.78 ATP + 5'-dephospho-DNA single stranded DNA Rattus norvegicus ADP + 5'-phospho-DNA UDP, ADP, GDP and CDP support the reverse reaction r
2.7.1.78 ATP + 5'-dephospho-DNA native and heat denatured DNA Rattus norvegicus ADP + 5'-phospho-DNA UDP, ADP, GDP and CDP support the reverse reaction r
2.7.1.78 ATP + 5'-dephospho-RNA no activity Rattus norvegicus ADP + 5'-phospho-RNA
-
 ?
2.7.1.78 ATP + synthetic oligonucleotide no activity with low molecular weight oligonucleotides Rattus norvegicus ADP + oligonucleotide 5'-phosphate
-
 ?
2.7.1.78 ATP + synthetic oligonucleotide oligodeoxynucleotides of chain length above 10-12 residues Rattus norvegicus ADP + oligonucleotide 5'-phosphate
-
 ?
2.7.1.78 CTP + 5'-dephospho-DNA UDP, ADP, GDP and CDP support the reverse reaction Rattus norvegicus CDP + 5'-phospho-DNA
-
 r
2.7.1.78 GTP + 5'-dephospho-DNA UDP, ADP, GDP and CDP support the reverse reaction Rattus norvegicus GDP + 5'-phospho-DNA
-
 r
2.7.1.78 UTP + 5'-dephospho-DNA UDP, ADP, GDP and CDP support the reverse reaction Rattus norvegicus UDP + 5'-phospho-DNA
-
 r

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.7.1.78 65
-
 quick inactivation Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.78 5.5
-
 sharp maximum Rattus norvegicus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.7.1.78 5 6.5 pH 5: about 50% of activity maximum, pH 6.5: about 35% of activity maximum Rattus norvegicus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
2.7.1.78 5.5
-
 37°C, unstable below Rattus norvegicus

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.1.78 ATP dependent on Rattus norvegicus
2.7.1.78 CDP
-
 Rattus norvegicus
2.7.1.78 CTP
-
 Rattus norvegicus
2.7.1.78 GDP
-
 Rattus norvegicus
2.7.1.78 GTP
-
 Rattus norvegicus
2.7.1.78 UDP
-
 Rattus norvegicus
2.7.1.78 UTP
-
 Rattus norvegicus