Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Billich, A.; Witzel, H.
    Nucleoside phosphotransferase from malt sprouts. III. Studies on metal ion requirement, solvent effects, kinetics and reaction mechanism (1986), Biol. Chem. Hoppe-Seyler, 367, 291-300.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.1.77 acetone 25%, 2.45fold stimulation Hordeum vulgare
2.7.1.77 acetonitrile 8%, 2.4fold stimulation Hordeum vulgare
2.7.1.77 Dimethyl formamide 5%, 1.8fold, stimulation Hordeum vulgare
2.7.1.77 dimethyl sulfoxide 20%, 1.8fold stimulation Hordeum vulgare
2.7.1.77 glycerol 60%, 1.7fold stimulation Hordeum vulgare
2.7.1.77 methanol 30%, 2.3fold stimulation Hordeum vulgare

General Stability

EC Number General Stability Organism
2.7.1.77 storage in 20% acetone for 1 week without loss of activity Hordeum vulgare

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.77 arsenate strong competitive inhibitor Hordeum vulgare
2.7.1.77 cAMP strong competitive inhibitor Hordeum vulgare
2.7.1.77 EDTA cAMP and arsenate can reduce the rate of inactivation Hordeum vulgare

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.77 Co2+ can restore activity of the metal-free apoenzyme Hordeum vulgare
2.7.1.77 Cu2+ can restore activity of the metal-free apoenzyme to a minor extent Hordeum vulgare
2.7.1.77 Fe2+ can restore activity of the metal-free apoenzyme to a minor extent Hordeum vulgare
2.7.1.77 Mg2+ contains one Mg2+ per dimeric enzyme molecule, the metal-free apoenzyme is inactive Hordeum vulgare
2.7.1.77 Ni2+ can restore activity of the metal-free apoenzyme to a minor extent Hordeum vulgare
2.7.1.77 Zn2+ can restore activity of the metal-free apoenzyme to a minor extent Hordeum vulgare

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.77 Hordeum vulgare
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.1.77 sprout
-
 Hordeum vulgare
-

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.7.1.77 additional information
-
 thermal stability is reduced when metal is removed but can be restored by addition of Mg2+ Hordeum vulgare

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.77 5.5
-
-
 Hordeum vulgare