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Literature summary extracted from

  • Billich, A.; Stockhowe, U.; Witzel, H.
    Nucleoside phosphotransferase from malt sprouts. I. Isolation, characterization and specificity of the enzyme (1986), Biol. Chem. Hoppe-Seyler, 367, 267-278.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.77 0.018
-
 5'-AMP pH 5.5 Hordeum vulgare
2.7.1.77 0.023
-
 3'-AMP pH 5.5 Hordeum vulgare
2.7.1.77 0.09
-
 5'-UMP pH 5.5 Hordeum vulgare
2.7.1.77 0.092
-
 3'-UMP pH 5.5 Hordeum vulgare
2.7.1.77 0.22
-
 adenosine 5'-phosphorothionate pH 5.5 Hordeum vulgare
2.7.1.77 0.26
-
 2-naphthyl phosphate pH 5.5 Hordeum vulgare
2.7.1.77 0.28
-
 4-nitrophenyl phosphate pH 5.5 Hordeum vulgare
2.7.1.77 0.44
-
 1-naphthyl phosphate
-
 Hordeum vulgare

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.77 26300
-
 x * 26300, SDS-PAGE Hordeum vulgare
2.7.1.77 55000
-
 gel filtration Hordeum vulgare

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.77 Hordeum vulgare
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
2.7.1.77 glycoprotein 1.2-1.4% carbohydrate, protein contains 3-4 carbohydrate residues Hordeum vulgare

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.77
-
 Hordeum vulgare

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.1.77 sprout
-
 Hordeum vulgare
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.77 123
-
-
 Hordeum vulgare

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.77 1-naphthyl phosphate + uridine
-
 Hordeum vulgare ?
-
 ?
2.7.1.77 2-naphthyl phosphate + uridine
-
 Hordeum vulgare ?
-
 ?
2.7.1.77 3'-AMP + uridine
-
 Hordeum vulgare adenosine + UMP
-
 ?
2.7.1.77 3'-UMP + uridine
-
 Hordeum vulgare ?
-
 ?
2.7.1.77 4-nitrophenyl phosphate + 1-(beta-D-ribofuranosyl)-2(1H)pyridone
-
 Hordeum vulgare 4-nitrophenol + 1-(beta-D-ribofuranosyl)-2(1H)pyridone 5'-phosphate
-
 ?
2.7.1.77 4-nitrophenyl phosphate + 1-(beta-D-ribofuranosyl)-4(1H)pyridone
-
 Hordeum vulgare 4-nitrophenol + 1-(beta-D-ribofuranosyl)-4(1H)pyridone 5'-phosphate
-
 ?
2.7.1.77 4-nitrophenyl phosphate + 2'-bromo-2'-deoxyuridine
-
 Hordeum vulgare 4-nitrophenol + 2'-bromo-2'-deoxyuridine 5'-phosphate
-
 ?
2.7.1.77 4-nitrophenyl phosphate + 2'-deoxy-2'-methyluridine
-
 Hordeum vulgare 4-nitrophenol + 2'-deoxy-2'-methyluridine 5'-phosphate
-
 ?
2.7.1.77 4-nitrophenyl phosphate + 2'-deoxyadenosine
-
 Hordeum vulgare 4-nitrophenol + 2'-deoxyadenosine 5'-phosphate
-
 ?
2.7.1.77 4-nitrophenyl phosphate + 2'-O-methylcytidine
-
 Hordeum vulgare 4-nitrophenol + 2'-O-methylcytidine 5'-phosphate
-
 ?
2.7.1.77 4-nitrophenyl phosphate + 2-thiouridine
-
 Hordeum vulgare 4-nitrophenol + 2-thiouridine 5'phosphate
-
 ?
2.7.1.77 4-nitrophenyl phosphate + 3'-deoxy-3'-methyluridine
-
 Hordeum vulgare 4-nitrophenol + 3'-deoxy-3'-methyluridine 5'-phosphate
-
 ?
2.7.1.77 4-nitrophenyl phosphate + 3'-deoxyadenosine
-
 Hordeum vulgare 4-nitrophenol + 3'-deoxyadenosine 5'phosphate
-
 ?
2.7.1.77 4-nitrophenyl phosphate + 3'-O-methylcytidine
-
 Hordeum vulgare 4-nitrophenol + 3'-O-methylcytidine 5'phosphate
-
 ?
2.7.1.77 4-nitrophenyl phosphate + 3-(beta-D-ribofuranosyl)uric acid
-
 Hordeum vulgare 4-nitrophenol + 3-(beta-D-ribofuranosyl)uric acid 5'-phosphate
-
 ?
2.7.1.77 4-nitrophenyl phosphate + 5-bromouridine
-
 Hordeum vulgare 4-nitrophenol + 5-bromouridine 5'phosphate
-
 ?
2.7.1.77 4-nitrophenyl phosphate + 5-chlorouridine
-
 Hordeum vulgare 4-nitrophenol + 5-chlorouridine 5'-phosphate
-
 ?
2.7.1.77 4-nitrophenyl phosphate + 5-hydroxyuridine
-
 Hordeum vulgare 4-nitrophenol + 5-hydroxyuridine 5'phosphate
-
 ?
2.7.1.77 4-nitrophenyl phosphate + 5-iodouridine
-
 Hordeum vulgare 4-nitrophenol + 5-iodouridine 5'-phosphate
-
 ?
2.7.1.77 4-nitrophenyl phosphate + 6-chloro-9-(beta-D-ribofuranosyl)purine
-
 Hordeum vulgare 4-nitrophenol + 6-chloro-9-(beta-D-ribofuranosyl)purine 5'-phosphate
-
 ?
2.7.1.77 4-nitrophenyl phosphate + 7-methylguanosine
-
 Hordeum vulgare 4-nitrophenol + 7-methylguanosine 5'-phosphate
-
 ?
2.7.1.77 4-nitrophenyl phosphate + 9-(beta-D-ribofuranosyl)purine
-
 Hordeum vulgare 4-nitrophenol + 9-(beta-D-ribofuranosyl)purine 5'-phosphate
-
 ?
2.7.1.77 4-nitrophenyl phosphate + adenosine
-
 Hordeum vulgare 5'-AMP + 4-nitrophenol
-
 ?
2.7.1.77 4-nitrophenyl phosphate + beta-pseudouridine
-
 Hordeum vulgare 4-nitrophenol + beta-pseudouridine 5'-phosphate
-
 ?
2.7.1.77 4-nitrophenyl phosphate + deazauridine
-
 Hordeum vulgare 4-nitrophenol + deazauridine 5'-phosphate
-
 ?
2.7.1.77 4-nitrophenyl phosphate + formycin B
-
 Hordeum vulgare 4-nitrophenol + ?
-
 ?
2.7.1.77 4-nitrophenyl phosphate + inosine
-
 Hordeum vulgare 4-nitrophenol + IMP
-
 ?
2.7.1.77 4-nitrophenyl phosphate + thymidine 3'-[(4-nitrophenyl)-phosphate]
-
 Hordeum vulgare 4-nitrophenol + thymidine 3'-[(4-nitrophenyl)-phosphate] 5'-phosphate
-
 ?
2.7.1.77 4-nitrophenyl phosphate + uridine
-
 Hordeum vulgare 4-nitrophenol + 5'-UMP
-
 ?
2.7.1.77 4-nitrophenyl phosphate + xanthosine
-
 Hordeum vulgare 4-nitrophenol + XMP
-
 ?
2.7.1.77 adenosine 5'-phosphorothionate + H2O
-
 Hordeum vulgare ?
-
 ?
2.7.1.77 additional information
-
 Hordeum vulgare ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.1.77 ? x * 26300, SDS-PAGE Hordeum vulgare

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.7.1.77 0.4
-
 adenosine-5'-phosphorothionate pH 5.5, phosphohydrolase activity Hordeum vulgare
2.7.1.77 2.6
-
 1-naphthyl phosphate pH 5.5, phosphohydrolase activity Hordeum vulgare
2.7.1.77 6.8
-
 3'-AMP pH 5.5, phosphohydrolase activity Hordeum vulgare
2.7.1.77 12
-
 3'-UMP pH 5.5 Hordeum vulgare
2.7.1.77 30
-
 2-naphthyl phosphate pH 5.5, phosphohydrolase activity Hordeum vulgare
2.7.1.77 41
-
 5'-AMP pH 5.5, phosphohydrolase activity Hordeum vulgare
2.7.1.77 67
-
 5'-UMP pH 5.5, phosphohydrolase activity Hordeum vulgare
2.7.1.77 77
-
 4-nitrophenyl phosphate pH 5.5, phosphohydrolase activity Hordeum vulgare

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.7.1.77 additional information
-
 additional information inhibitors of phosphohydrolase activity Hordeum vulgare