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Literature summary extracted from

  • Prasher, D.C.; Carr, M.C.; Ives, D.H.; Tsai, T.C.; Frey, P.A.
    Nucleoside phosphotransferase from barley. Characterization and evidence for ping pong kinetics involving phosphoryl enzyme (1982), J. Biol. Chem., 257, 4931-4939.
    View publication on PubMed

General Stability

EC Number General Stability Organism
2.7.1.77 withstands repeated freezing and thawing if the protein concentration is less than 0.5 mg/ml Hordeum vulgare

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.77 Ca2+ 2 mM, at least 80% inhibition of transphosphorylation Hordeum vulgare
2.7.1.77 Cd2+ 2 mM, at least 80% inhibition of transphosphorylation Hordeum vulgare
2.7.1.77 Cu2+ 2 mM, at least 80% inhibition of transphosphorylation Hordeum vulgare
2.7.1.77 EDTA 2 mM, 85% inhibition. 95% inhibition at 10 mM Hordeum vulgare
2.7.1.77 Hg2+ 2 mM, at least 80% inhibition of transphosphorylation Hordeum vulgare
2.7.1.77 Zn2+ 2 mM, 20% inhibition of transphosphorylation Hordeum vulgare

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.77 0.087
-
 deoxyadenosine pH 5.0, reaction with AMP Hordeum vulgare
2.7.1.77 0.412
-
 2'-deoxyguanosine pH 5.0, reaction with AMP Hordeum vulgare
2.7.1.77 0.89
-
 2'-deoxythymidine pH 5.0, reaction with AMP Hordeum vulgare
2.7.1.77 2.13
-
 guanosine pH 5.0, reaction with AMP Hordeum vulgare
2.7.1.77 3.93
-
 uridine pH 5.0, reaction with AMP Hordeum vulgare
2.7.1.77 4.15
-
 2'-deoxycytidine pH 5.0, reaction with AMP Hordeum vulgare
2.7.1.77 6.39
-
 cytidine pH 5.0, reaction with AMP Hordeum vulgare

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.77 Ba2+ 2 mM, activity with AMP and deoxythymidine is enhanced about 50%, no absolute requirement Hordeum vulgare
2.7.1.77 Co2+ 2 mM, activity with AMP and deoxythymidine is enhanced about 50%, no absolute requirement Hordeum vulgare
2.7.1.77 Mg2+ 2 mM, activity with AMP and deoxythymidine is enhanced about 50%, no absolute requirement Hordeum vulgare
2.7.1.77 Mg2+ 2 mM, with 0.5 mM AMP and deoxythymidine, 50% stimulation Hordeum vulgare

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.77 50000
-
 2 * 50000, SDS-PAGE Hordeum vulgare
2.7.1.77 53400
-
 non-denaturing PAGE Hordeum vulgare

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.77 Hordeum vulgare
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
2.7.1.77 glycoprotein carrying a variable amount of polysaccharide Hordeum vulgare

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.77
-
 Hordeum vulgare

Reaction

EC Number Reaction Comment Organism Reaction ID
2.7.1.77 a nucleotide + a 2'-deoxyribonucleoside = a nucleoside + a 2'-deoxyribonucleoside 5'-phosphate ping pong kinetics Hordeum vulgare
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.1.77 seedling
-
 Hordeum vulgare
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.77 180
-
-
 Hordeum vulgare

Storage Stability

EC Number Storage Stability Organism
2.7.1.77 -20°C, stable for at least 6 months Hordeum vulgare
2.7.1.77 0°C, 0.01% Triton X-100 or 0.25 M sodium acetate, pH 5, 44 h, phosphotransferase activity decreases 20% and 50% respectively Hordeum vulgare

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.77 3'-AMP + 2'-deoxycytidine
-
 Hordeum vulgare dCMP + adenosine
-
 ?
2.7.1.77 3'-AMP + 2'-deoxyguanosine
-
 Hordeum vulgare dGMP + adenosine
-
 ?
2.7.1.77 3'-AMP + 2'-deoxythymidine
-
 Hordeum vulgare dTMP + adenosine
-
 ?
2.7.1.77 3'-AMP + cytidine
-
 Hordeum vulgare CMP + adenosine
-
 ?
2.7.1.77 3'-AMP + guanosine
-
 Hordeum vulgare GMP + adenosine
-
 ?
2.7.1.77 4-nitrophenyl phosphate + 2'-deoxyadenosine
-
 Hordeum vulgare 4-nitrophenol + 2'-deoxyadenosine 5'-phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.1.77 dimer 2 * 50000, SDS-PAGE Hordeum vulgare

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.7.1.77 additional information
-
 additional information
-
 Hordeum vulgare

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.77 5.5
-
 phosphatase activity Hordeum vulgare
2.7.1.77 6.5
-
 transferase activity Hordeum vulgare

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.7.1.77 4.5 8.5 pH 4.5: about 65% of maximal activity, pH 8.5: about 50% of maximal activity Hordeum vulgare