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Literature summary extracted from

  • Chao, H.M.
    Nucleoside phosphotransferase from Erwinia herbicola, a new membrane-bound enzyme (1976), J. Biol. Chem., 251, 2330-2333.
    View publication on PubMed

General Stability

EC Number General Stability Organism
2.7.1.77 does not lose acttivity on lyophilization Pantoea agglomerans
2.7.1.77 not stable to SDS Pantoea agglomerans
2.7.1.77 stable in presence of 2 M urea, but not 4 M urea Pantoea agglomerans
2.7.1.77 the enzyme can be isolated and handled only in the presence of detergents Pantoea agglomerans
2.7.1.77 tolerates repeated freezing and thawing Pantoea agglomerans

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.77 Ca2+
-
 Pantoea agglomerans
2.7.1.77 Co2+
-
 Pantoea agglomerans
2.7.1.77 Cu2+
-
 Pantoea agglomerans
2.7.1.77 EDTA 1 mM, 53% inhibition of transferase activity Pantoea agglomerans
2.7.1.77 Zn2+
-
 Pantoea agglomerans

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.1.77 membrane bound to Pantoea agglomerans 16020
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.77 Mg2+ 10 mM MgCl2, 20% enhancement of transferase activity with p-nitrophenol as the donor, no absolute requirement for metal ion Pantoea agglomerans

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.77 additional information
-
 the purified enzyme shows two separable bands in SDS-PAGE: 70000 Da and 24000 Da Pantoea agglomerans
2.7.1.77 68000
-
 sucrose density gradient sedimentation Pantoea agglomerans

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.77 Pantoea agglomerans
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.77
-
 Pantoea agglomerans

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.77 12
-
-
 Pantoea agglomerans

Storage Stability

EC Number Storage Stability Organism
2.7.1.77 4°C, 10% glycerol, pH 7.4, stable for at least 2 months Pantoea agglomerans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.77 3'-CMP + thymidine 20% of the activity with 5'-dAMP or 5'-dTMP Pantoea agglomerans cytosine + TMP
-
 ?
2.7.1.77 3'-dAMP + thymidine 56% of the activity with 5'-dAMP or 5'-dTMP Pantoea agglomerans TMP + 3'-deoxyadenosine
-
 ?
2.7.1.77 3'-TMP + thymidine 19% of the activity with 5'-dAMP or 5'-dTMP Pantoea agglomerans thymidine + TMP
-
 ?
2.7.1.77 4-nitrophenyl phosphate + 2'-deoxyadenosine
-
 Pantoea agglomerans 2'-deoxyadenosine 5'-phosphate + 2'-deoxyadenosine 3'-phosphate + 4-nitrophenol 5'- and 3'-nucleotide products in the approximate ratio of 2:1 ?
2.7.1.77 4-nitrophenyl phosphate + adenosine
-
 Pantoea agglomerans 5'-AMP + 4-nitrophenol
-
 ?
2.7.1.77 4-nitrophenyl phosphate + thymidine 24% of the activity with 5'-dAMP or 5'-dTMP Pantoea agglomerans 4-nitrophenol + TMP
-
 ?
2.7.1.77 5'-AMP + thymidine 72% of the activity with 5'-dAMP or 5'-dTMP Pantoea agglomerans adenosine + TMP
-
 ?
2.7.1.77 5'-CMP + thymidine 92% of the activity with 5'-dAMP or 5'-dTMP Pantoea agglomerans cytidine + TMP
-
 ?
2.7.1.77 5'-dAMP + thymidine
-
 Pantoea agglomerans 5'-deoxyadenosine + TMP
-
 ?
2.7.1.77 5'-dCMP + thymidine 46% of the activity with 5'-dAMP or 5'-dTMP Pantoea agglomerans 2'-deoxycytidine + TMP
-
 ?
2.7.1.77 5'-dGMP + thymidine 65% of the activity with 5'-dAMP or 5'-dTMP Pantoea agglomerans TMP + 2'-deoxyguanosine
-
 ?
2.7.1.77 5'-dTMP + thymidine
-
 Pantoea agglomerans 5'-deoxythymidine + TMP
-
 ?
2.7.1.77 5'-GMP + thymidine 61% of the activity with 5'-dAMP or 5'-dTMP Pantoea agglomerans TMP + guanosine
-
 ?
2.7.1.77 adenosine 3',5'-bisphosphate + thymidine 30% of the activity with 5'-dAMP or 5'-dTMP Pantoea agglomerans adenosine 3'-phosphate + TMP
-
 ?
2.7.1.77 ADP + thymidine 50% of the activity with 5'-dAMP or 5'-dTMP Pantoea agglomerans TMP + AMP
-
 ?
2.7.1.77 ATP + thymidine 18% of the activity with 5'-dAMP or 5'-dTMP Pantoea agglomerans ADP + TMP
-
 ?
2.7.1.77 D-ribose 5-phosphate + thymidine 13% of the activity with 5'-dAMP or 5'-dTMP Pantoea agglomerans D-ribose + TMP
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.7.1.77 50
-
 5 min, stable Pantoea agglomerans