BRENDA - Enzyme Database

Nucleoside phosphotransferase from Erwinia herbicola, a new membrane-bound enzyme

Chao, H.M.; J. Biol. Chem. 251, 2330-2333 (1976)

Data extracted from this reference:

General Stability
EC Number
General Stability
Organism
2.7.1.77
does not lose acttivity on lyophilization
Pantoea agglomerans
2.7.1.77
not stable to SDS
Pantoea agglomerans
2.7.1.77
stable in presence of 2 M urea, but not 4 M urea
Pantoea agglomerans
2.7.1.77
the enzyme can be isolated and handled only in the presence of detergents
Pantoea agglomerans
2.7.1.77
tolerates repeated freezing and thawing
Pantoea agglomerans
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
2.7.1.77
Ca2+
-
Pantoea agglomerans
2.7.1.77
Co2+
-
Pantoea agglomerans
2.7.1.77
Cu2+
-
Pantoea agglomerans
2.7.1.77
EDTA
1 mM, 53% inhibition of transferase activity
Pantoea agglomerans
2.7.1.77
Zn2+
-
Pantoea agglomerans
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.7.1.77
membrane
bound to
Pantoea agglomerans
16020
-
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
2.7.1.77
Mg2+
10 mM MgCl2, 20% enhancement of transferase activity with p-nitrophenol as the donor, no absolute requirement for metal ion
Pantoea agglomerans
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.7.1.77
additional information
-
the purified enzyme shows two separable bands in SDS-PAGE: 70000 Da and 24000 Da
Pantoea agglomerans
2.7.1.77
68000
-
sucrose density gradient sedimentation
Pantoea agglomerans
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.7.1.77
Pantoea agglomerans
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
2.7.1.77
-
Pantoea agglomerans
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.7.1.77
12
-
-
Pantoea agglomerans
Storage Stability
EC Number
Storage Stability
Organism
2.7.1.77
4°C, 10% glycerol, pH 7.4, stable for at least 2 months
Pantoea agglomerans
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.1.77
3'-CMP + thymidine
20% of the activity with 5'-dAMP or 5'-dTMP
641993
Pantoea agglomerans
cytosine + TMP
-
-
-
?
2.7.1.77
3'-dAMP + thymidine
56% of the activity with 5'-dAMP or 5'-dTMP
641993
Pantoea agglomerans
TMP + 2'-deoxyadenosine
-
-
-
?
2.7.1.77
3'-TMP + thymidine
19% of the activity with 5'-dAMP or 5'-dTMP
641993
Pantoea agglomerans
thymidine + TMP
-
-
-
?
2.7.1.77
4-nitrophenyl phosphate + 2'-deoxyadenosine
-
641993
Pantoea agglomerans
5'-dAMP + 3'-dAMP + nitrophenol
5'- and 3'-nucleotide products in the approximate ratio of 2:1
641993
Pantoea agglomerans
?
2.7.1.77
4-nitrophenyl phosphate + adenosine
-
641993
Pantoea agglomerans
5'-AMP + 4-nitrophenol
-
641993
Pantoea agglomerans
?
2.7.1.77
4-nitrophenyl phosphate + thymidine
24% of the activity with 5'-dAMP or 5'-dTMP
641993
Pantoea agglomerans
4-nitrophenol + TMP
-
-
-
?
2.7.1.77
5'-AMP + thymidine
72% of the activity with 5'-dAMP or 5'-dTMP
641993
Pantoea agglomerans
adenosine + TMP
-
-
-
?
2.7.1.77
5'-CMP + thymidine
92% of the activity with 5'-dAMP or 5'-dTMP
641993
Pantoea agglomerans
cytidine + TMP
-
-
-
?
2.7.1.77
5'-dAMP + thymidine
-
641993
Pantoea agglomerans
5'-deoxyadenosine + TMP
-
-
-
?
2.7.1.77
5'-dCMP + thymidine
46% of the activity with 5'-dAMP or 5'-dTMP
641993
Pantoea agglomerans
2'-deoxycytidine + TMP
-
-
-
?
2.7.1.77
5'-dGMP + thymidine
65% of the activity with 5'-dAMP or 5'-dTMP
641993
Pantoea agglomerans
TMP + 2'-deoxyguanosine
-
-
-
?
2.7.1.77
5'-dTMP + thymidine
-
641993
Pantoea agglomerans
5'-deoxythymidine + TMP
-
-
-
?
2.7.1.77
5'-GMP + thymidine
61% of the activity with 5'-dAMP or 5'-dTMP
641993
Pantoea agglomerans
TMP + guanosine
-
-
-
?
2.7.1.77
adenosine 3',5'-bisphosphate + thymidine
30% of the activity with 5'-dAMP or 5'-dTMP
641993
Pantoea agglomerans
adenosine 3'-phosphate + TMP
-
-
-
?
2.7.1.77
ADP + thymidine
50% of the activity with 5'-dAMP or 5'-dTMP
641993
Pantoea agglomerans
TMP + AMP
-
-
-
?
2.7.1.77
ATP + thymidine
18% of the activity with 5'-dAMP or 5'-dTMP
641993
Pantoea agglomerans
ADP + TMP
-
-
-
?
2.7.1.77
D-ribose 5-phosphate + thymidine
13% of the activity with 5'-dAMP or 5'-dTMP
641993
Pantoea agglomerans
D-ribose + TMP
-
-
-
?
Temperature Stability [°C]
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
2.7.1.77
50
-
5 min, stable
Pantoea agglomerans
General Stability (protein specific)
EC Number
General Stability
Organism
2.7.1.77
does not lose acttivity on lyophilization
Pantoea agglomerans
2.7.1.77
not stable to SDS
Pantoea agglomerans
2.7.1.77
stable in presence of 2 M urea, but not 4 M urea
Pantoea agglomerans
2.7.1.77
the enzyme can be isolated and handled only in the presence of detergents
Pantoea agglomerans
2.7.1.77
tolerates repeated freezing and thawing
Pantoea agglomerans
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
2.7.1.77
Ca2+
-
Pantoea agglomerans
2.7.1.77
Co2+
-
Pantoea agglomerans
2.7.1.77
Cu2+
-
Pantoea agglomerans
2.7.1.77
EDTA
1 mM, 53% inhibition of transferase activity
Pantoea agglomerans
2.7.1.77
Zn2+
-
Pantoea agglomerans
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.7.1.77
membrane
bound to
Pantoea agglomerans
16020
-
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
2.7.1.77
Mg2+
10 mM MgCl2, 20% enhancement of transferase activity with p-nitrophenol as the donor, no absolute requirement for metal ion
Pantoea agglomerans
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.7.1.77
additional information
-
the purified enzyme shows two separable bands in SDS-PAGE: 70000 Da and 24000 Da
Pantoea agglomerans
2.7.1.77
68000
-
sucrose density gradient sedimentation
Pantoea agglomerans
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.7.1.77
-
Pantoea agglomerans
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.7.1.77
12
-
-
Pantoea agglomerans
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
2.7.1.77
4°C, 10% glycerol, pH 7.4, stable for at least 2 months
Pantoea agglomerans
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.1.77
3'-CMP + thymidine
20% of the activity with 5'-dAMP or 5'-dTMP
641993
Pantoea agglomerans
cytosine + TMP
-
-
-
?
2.7.1.77
3'-dAMP + thymidine
56% of the activity with 5'-dAMP or 5'-dTMP
641993
Pantoea agglomerans
TMP + 2'-deoxyadenosine
-
-
-
?
2.7.1.77
3'-TMP + thymidine
19% of the activity with 5'-dAMP or 5'-dTMP
641993
Pantoea agglomerans
thymidine + TMP
-
-
-
?
2.7.1.77
4-nitrophenyl phosphate + 2'-deoxyadenosine
-
641993
Pantoea agglomerans
5'-dAMP + 3'-dAMP + nitrophenol
5'- and 3'-nucleotide products in the approximate ratio of 2:1
641993
Pantoea agglomerans
?
2.7.1.77
4-nitrophenyl phosphate + adenosine
-
641993
Pantoea agglomerans
5'-AMP + 4-nitrophenol
-
641993
Pantoea agglomerans
?
2.7.1.77
4-nitrophenyl phosphate + thymidine
24% of the activity with 5'-dAMP or 5'-dTMP
641993
Pantoea agglomerans
4-nitrophenol + TMP
-
-
-
?
2.7.1.77
5'-AMP + thymidine
72% of the activity with 5'-dAMP or 5'-dTMP
641993
Pantoea agglomerans
adenosine + TMP
-
-
-
?
2.7.1.77
5'-CMP + thymidine
92% of the activity with 5'-dAMP or 5'-dTMP
641993
Pantoea agglomerans
cytidine + TMP
-
-
-
?
2.7.1.77
5'-dAMP + thymidine
-
641993
Pantoea agglomerans
5'-deoxyadenosine + TMP
-
-
-
?
2.7.1.77
5'-dCMP + thymidine
46% of the activity with 5'-dAMP or 5'-dTMP
641993
Pantoea agglomerans
2'-deoxycytidine + TMP
-
-
-
?
2.7.1.77
5'-dGMP + thymidine
65% of the activity with 5'-dAMP or 5'-dTMP
641993
Pantoea agglomerans
TMP + 2'-deoxyguanosine
-
-
-
?
2.7.1.77
5'-dTMP + thymidine
-
641993
Pantoea agglomerans
5'-deoxythymidine + TMP
-
-
-
?
2.7.1.77
5'-GMP + thymidine
61% of the activity with 5'-dAMP or 5'-dTMP
641993
Pantoea agglomerans
TMP + guanosine
-
-
-
?
2.7.1.77
adenosine 3',5'-bisphosphate + thymidine
30% of the activity with 5'-dAMP or 5'-dTMP
641993
Pantoea agglomerans
adenosine 3'-phosphate + TMP
-
-
-
?
2.7.1.77
ADP + thymidine
50% of the activity with 5'-dAMP or 5'-dTMP
641993
Pantoea agglomerans
TMP + AMP
-
-
-
?
2.7.1.77
ATP + thymidine
18% of the activity with 5'-dAMP or 5'-dTMP
641993
Pantoea agglomerans
ADP + TMP
-
-
-
?
2.7.1.77
D-ribose 5-phosphate + thymidine
13% of the activity with 5'-dAMP or 5'-dTMP
641993
Pantoea agglomerans
D-ribose + TMP
-
-
-
?
Temperature Stability [°C] (protein specific)
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
2.7.1.77
50
-
5 min, stable
Pantoea agglomerans