BRENDA - Enzyme Database

The regulatory properties of deoxyadenosine kinase

Krygier, V.; Momparler, R.L.; Biochim. Biophys. Acta 161, 578-580 (1968)

Data extracted from this reference:

General Stability
EC Number
General Stability
Organism
2.7.1.76
glycerol, 50%, or dithiothreitol stabilizes
Bos taurus
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
2.7.1.76
dADP
0.05 mM, 70% inhibition
Bos taurus
2.7.1.76
dAMP
0.2 mM, 36% inhibition
Bos taurus
2.7.1.76
dATP
0.05 mM, 68% inhibition
Bos taurus
2.7.1.76
dCDP
0.001 mM, 60% inhibition
Bos taurus
2.7.1.76
dCMP
0.01 mM, 88% inhibition
Bos taurus
2.7.1.76
dCTP
0.0005 mM, 59% inhibition
Bos taurus
2.7.1.76
deoxyguanosine
substrate deoxyadenosine
Bos taurus
2.7.1.76
dGDP
0.2 mM, 46% inhibition
Bos taurus
2.7.1.76
dGMP
0.5 mM, 32% inhibition
Bos taurus
2.7.1.76
dGTP
0.2 mM, 64% inhibition
Bos taurus
2.7.1.76
additional information
not inhibited by adenosine
Bos taurus
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.7.1.76
5
-
deoxyadenosine
pH 7.8, 37°C
Bos taurus
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
2.7.1.76
Mg2+
required for activity
Bos taurus
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.7.1.76
Bos taurus
-
calf
-
Purification (Commentary)
EC Number
Commentary
Organism
2.7.1.76
streptomycin sulfate, protamine sulfate, ammonium sulfate, Sephadex G-150
Bos taurus
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
2.7.1.76
thymus
-
Bos taurus
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.7.1.76
additional information
-
0.00006 mmol/ml min
Bos taurus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.1.76
ATP + deoxyadenosine
transfers a phospho group from specific nucleoside 5'-triphosphate donors to 5'-position of deoxyadenosine
641974
Bos taurus
ADP + dAMP
-
641974
Bos taurus
?
2.7.1.76
ATP + deoxyadenosine
no activity with adenosine and guanosine
641974
Bos taurus
ADP + dAMP
-
641974
Bos taurus
?
2.7.1.76
ATP + deoxyguanosine
-
641974
Bos taurus
ADP + dGMP
-
641974
Bos taurus
?
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
2.7.1.76
37
-
assay at
Bos taurus
General Stability (protein specific)
EC Number
General Stability
Organism
2.7.1.76
glycerol, 50%, or dithiothreitol stabilizes
Bos taurus
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
2.7.1.76
dADP
0.05 mM, 70% inhibition
Bos taurus
2.7.1.76
dAMP
0.2 mM, 36% inhibition
Bos taurus
2.7.1.76
dATP
0.05 mM, 68% inhibition
Bos taurus
2.7.1.76
dCDP
0.001 mM, 60% inhibition
Bos taurus
2.7.1.76
dCMP
0.01 mM, 88% inhibition
Bos taurus
2.7.1.76
dCTP
0.0005 mM, 59% inhibition
Bos taurus
2.7.1.76
deoxyguanosine
substrate deoxyadenosine
Bos taurus
2.7.1.76
dGDP
0.2 mM, 46% inhibition
Bos taurus
2.7.1.76
dGMP
0.5 mM, 32% inhibition
Bos taurus
2.7.1.76
dGTP
0.2 mM, 64% inhibition
Bos taurus
2.7.1.76
additional information
not inhibited by adenosine
Bos taurus
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.7.1.76
5
-
deoxyadenosine
pH 7.8, 37°C
Bos taurus
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
2.7.1.76
Mg2+
required for activity
Bos taurus
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.7.1.76
streptomycin sulfate, protamine sulfate, ammonium sulfate, Sephadex G-150
Bos taurus
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
2.7.1.76
thymus
-
Bos taurus
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.7.1.76
additional information
-
0.00006 mmol/ml min
Bos taurus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.1.76
ATP + deoxyadenosine
transfers a phospho group from specific nucleoside 5'-triphosphate donors to 5'-position of deoxyadenosine
641974
Bos taurus
ADP + dAMP
-
641974
Bos taurus
?
2.7.1.76
ATP + deoxyadenosine
no activity with adenosine and guanosine
641974
Bos taurus
ADP + dAMP
-
641974
Bos taurus
?
2.7.1.76
ATP + deoxyguanosine
-
641974
Bos taurus
ADP + dGMP
-
641974
Bos taurus
?
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
2.7.1.76
37
-
assay at
Bos taurus