Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Someya, H.; Shaddix, S.C.; Tiwari, K.N.; Secrist, J.A., 3rd; Parker, W.B.
    Phosphorylation of 4'-thio-beta-D-arabinofuranosylcytosine and its analogs by human deoxycytidine kinase (2003), J. Pharmacol. Exp. Ther., 304, 1314-1322.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.74 4'-thio-beta-D-arabinofuranosylcytosine IC50 0.000024 mM Homo sapiens
2.7.1.74 5-chloro-4'-thio-beta-D-arabinofuranosylcytosine IC50 0.051 mM Homo sapiens
2.7.1.74 5-fluoro-4'-thio-beta-D-arabinofuranosylcytosine IC50 0.0000020 mM Homo sapiens
2.7.1.74 cytosine arabinoside IC50 0.0000055 mM Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.74 0.00013
-
 deoxycytidine cosubstrate UTP 37°C, pH 8.0 Homo sapiens
2.7.1.74 0.00032
-
 4'-thio-2'-deoxycytidine cosubstrate ATP 37°C, pH 8.0 Homo sapiens
2.7.1.74 0.00041
-
 beta-D-arabinofuranosylcytosine cosubstrate UTP 37°C, pH 8.0 Homo sapiens
2.7.1.74 0.00057
-
 deoxycytidine cosubstrate ATP 37°C, pH 8.0 Homo sapiens
2.7.1.74 0.015
-
 beta-D-arabinofuranosylcytosine cosubstrate ATP 37°C, pH 8.0 Homo sapiens
2.7.1.74 0.02
-
 4'-thio-beta-D-arabinofuranosylcytosine cosubstrate UTP 37°C, pH 8.0 Homo sapiens
2.7.1.74 0.088
-
 4'-thio-beta-D-arabinofuranosylcytosine cosubstrate ATP 37°C, pH 8.0 Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.74 UTP + deoxycytidine Homo sapiens UTP is physiological phosphate donor UDP + dCMP
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.74 Homo sapiens
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.74 4'-thio-2'-deoxycytidine + UTP
-
 Homo sapiens UDP + 4'-thio-2'-deoxycytidine 5'-phosphate
-
 ?
2.7.1.74 4'-thio-beta-D-arabinofuranosylcytosine + UTP
-
 Homo sapiens UDP + 4'-thio-beta-D-arabinofuranosylcytosine 5'-phosphate
-
 ?
2.7.1.74 NTP + cytosine arabinoside
-
 Homo sapiens NDP + cytosine arabinoside 5'-monophosphate
-
 ?
2.7.1.74 NTP + deoxycytidine
-
 Homo sapiens NDP + dCMP
-
 ?
2.7.1.74 UTP + deoxycytidine UTP is physiological phosphate donor Homo sapiens UDP + dCMP
-
 ?

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
2.7.1.74 0.000002
-
 IC50 0.0000020 mM Homo sapiens 5-fluoro-4'-thio-beta-D-arabinofuranosylcytosine
2.7.1.74 0.0000055
-
 IC50 0.0000055 mM Homo sapiens cytosine arabinoside
2.7.1.74 0.000024
-
 IC50 0.000024 mM Homo sapiens 4'-thio-beta-D-arabinofuranosylcytosine
2.7.1.74 0.051
-
 IC50 0.051 mM Homo sapiens 5-chloro-4'-thio-beta-D-arabinofuranosylcytosine