BRENDA - Enzyme Database

Deoxynucleoside kinases from Lactobacilli. Separate interacting sites for deoxycytidine and deoxyadenosine

Deibler, R.D.; Reznik, R.B.; Ives, D.H.; J. Biol. Chem. 252, 8240-8244 (1977)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
2.7.1.76
2'-O-Cyclocytidine
activation
Lactobacillus acidophilus
2.7.1.76
5-Fluoro-2'-deoxycytidine
0.4 mM, 275% activation of deoxyadenosine phosphorylation, half-maximal activation at 0.028 mM
Lactobacillus acidophilus
2.7.1.76
cytosine arabinoside
activation
Lactobacillus acidophilus
2.7.1.76
deoxycytidine
30 mM, 584% activation of deoxyadenosine phosphorylation, half-maximal activation at 0.1 mM
Lactobacillus acidophilus
2.7.1.76
deoxycytidine monophosphate
10 mM, 409% activation of deoxyadenosine phosphorylation, half-maximal activation at 1.6 mM
Lactobacillus acidophilus
2.7.1.76
deoxyguanosine
5 mM, 330% activation of deoxyadenosine phosphorylation, half-maximal activation at 2.2 mM
Lactobacillus acidophilus
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
2.7.1.74
5-Fluoro-2'-deoxycytidine
-
Lactobacillus acidophilus
2.7.1.74
cytidine
-
Lactobacillus acidophilus
2.7.1.74
dATP
not
Lactobacillus acidophilus
2.7.1.74
dCTP
ATP as substrate; moderate phosphate donor in the absence of ATP
Lactobacillus acidophilus
2.7.1.74
deoxyadenosine
cytosolic isozyme I, weak; deoxycytidine; kinetics
Lactobacillus acidophilus
2.7.1.74
deoxyguanosine
-
Lactobacillus acidophilus
2.7.1.74
dGTP
ATP as substrate
Lactobacillus acidophilus
2.7.1.74
dTTP
ATP as substrate; weak
Lactobacillus acidophilus
2.7.1.74
Trypsin
substrates or dNTP protect corresponding active site
Lactobacillus acidophilus
2.7.1.76
adenine arabinoside
-
Lactobacillus acidophilus
2.7.1.76
adenosine
-
Lactobacillus acidophilus
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.7.1.74
additional information
-
additional information
kinetic study
Lactobacillus acidophilus
2.7.1.76
additional information
-
additional information
kinetic study
Lactobacillus acidophilus
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
2.7.1.74
Mg2+
-
Lactobacillus acidophilus
2.7.1.76
Mg2+
required for activity
Lactobacillus acidophilus
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.7.1.74
Lactobacillus acidophilus
-
-
-
2.7.1.76
Lactobacillus acidophilus
-
-
-
Reaction
EC Number
Reaction
Commentary
Organism
2.7.1.74
NTP + deoxycytidine = NDP + dCMP
mechanism
Lactobacillus acidophilus
2.7.1.76
ATP + 2'-deoxyadenosine = ADP + dAMP
random sequential substrate addition
Lactobacillus acidophilus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.1.74
ATP + 2'-deoxycytidine
-
641950
Lactobacillus acidophilus
ADP + 5'-dCMP
-
-
-
?
2.7.1.74
dCTP + 2'-deoxycytidine
only in the absence of ATP
641950
Lactobacillus acidophilus
dCDP + 2'-deoxy-CMP
-
-
-
?
2.7.1.74
additional information
enzyme has two separate active sites for deoxycytidine and deoxyadenosine activity
641950
Lactobacillus acidophilus
?
-
-
-
-
2.7.1.74
NTP + deoxyadenosine
-
641950
Lactobacillus acidophilus
NDP + dAMP
-
-
-
?
2.7.1.74
NTP + deoxycytidine
-
641950
Lactobacillus acidophilus
NDP + dCMP
-
-
-
?
2.7.1.76
ATP + deoxyadenosine
enzyme bears two separate but interacting active sites for deoxyadenosine and deoxycytidine kinase activity
641950
Lactobacillus acidophilus
ADP + dAMP
-
641950
Lactobacillus acidophilus
?
2.7.1.76
ATP + deoxycytidine
-
641950
Lactobacillus acidophilus
ADP + dCMP
-
641950
Lactobacillus acidophilus
?
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
2.7.1.76
20
-
assay at
Lactobacillus acidophilus
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
2.7.1.76
2'-O-Cyclocytidine
activation
Lactobacillus acidophilus
2.7.1.76
5-Fluoro-2'-deoxycytidine
0.4 mM, 275% activation of deoxyadenosine phosphorylation, half-maximal activation at 0.028 mM
Lactobacillus acidophilus
2.7.1.76
cytosine arabinoside
activation
Lactobacillus acidophilus
2.7.1.76
deoxycytidine
30 mM, 584% activation of deoxyadenosine phosphorylation, half-maximal activation at 0.1 mM
Lactobacillus acidophilus
2.7.1.76
deoxycytidine monophosphate
10 mM, 409% activation of deoxyadenosine phosphorylation, half-maximal activation at 1.6 mM
Lactobacillus acidophilus
2.7.1.76
deoxyguanosine
5 mM, 330% activation of deoxyadenosine phosphorylation, half-maximal activation at 2.2 mM
Lactobacillus acidophilus
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
2.7.1.74
5-Fluoro-2'-deoxycytidine
-
Lactobacillus acidophilus
2.7.1.74
cytidine
-
Lactobacillus acidophilus
2.7.1.74
dATP
not
Lactobacillus acidophilus
2.7.1.74
dCTP
ATP as substrate; moderate phosphate donor in the absence of ATP
Lactobacillus acidophilus
2.7.1.74
deoxyadenosine
cytosolic isozyme I, weak; deoxycytidine; kinetics
Lactobacillus acidophilus
2.7.1.74
deoxyguanosine
-
Lactobacillus acidophilus
2.7.1.74
dGTP
ATP as substrate
Lactobacillus acidophilus
2.7.1.74
dTTP
ATP as substrate; weak
Lactobacillus acidophilus
2.7.1.74
Trypsin
substrates or dNTP protect corresponding active site
Lactobacillus acidophilus
2.7.1.76
adenine arabinoside
-
Lactobacillus acidophilus
2.7.1.76
adenosine
-
Lactobacillus acidophilus
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.7.1.74
additional information
-
additional information
kinetic study
Lactobacillus acidophilus
2.7.1.76
additional information
-
additional information
kinetic study
Lactobacillus acidophilus
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
2.7.1.74
Mg2+
-
Lactobacillus acidophilus
2.7.1.76
Mg2+
required for activity
Lactobacillus acidophilus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.1.74
ATP + 2'-deoxycytidine
-
641950
Lactobacillus acidophilus
ADP + 5'-dCMP
-
-
-
?
2.7.1.74
dCTP + 2'-deoxycytidine
only in the absence of ATP
641950
Lactobacillus acidophilus
dCDP + 2'-deoxy-CMP
-
-
-
?
2.7.1.74
additional information
enzyme has two separate active sites for deoxycytidine and deoxyadenosine activity
641950
Lactobacillus acidophilus
?
-
-
-
-
2.7.1.74
NTP + deoxyadenosine
-
641950
Lactobacillus acidophilus
NDP + dAMP
-
-
-
?
2.7.1.74
NTP + deoxycytidine
-
641950
Lactobacillus acidophilus
NDP + dCMP
-
-
-
?
2.7.1.76
ATP + deoxyadenosine
enzyme bears two separate but interacting active sites for deoxyadenosine and deoxycytidine kinase activity
641950
Lactobacillus acidophilus
ADP + dAMP
-
641950
Lactobacillus acidophilus
?
2.7.1.76
ATP + deoxycytidine
-
641950
Lactobacillus acidophilus
ADP + dCMP
-
641950
Lactobacillus acidophilus
?
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
2.7.1.76
20
-
assay at
Lactobacillus acidophilus