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Literature summary extracted from

  • Bohman, C.; Eriksson, S.
    Deoxycytidine kinase from human leukemic spleen: preparation and characteristics of homogeneous enzyme (1988), Biochemistry, 27, 4258-4265.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.1.74 dithiothreitol activation Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.74 dCTP kinetics Homo sapiens
2.7.1.74 deoxyadenosine deoxycytidine Homo sapiens
2.7.1.74 deoxycytidine deoxyadenosine as substrate; deoxyguanosine as substrate Homo sapiens
2.7.1.74 deoxyguanosine deoxycytidine as substrate Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.74 additional information
-
 additional information
-
 Homo sapiens
2.7.1.74 0.0008 0.0015 deoxycytidine pH 8.0, 37°C Homo sapiens
2.7.1.74 0.02 0.04 cytosine arabinoside pH 8.0, 37°C Homo sapiens
2.7.1.74 0.12
-
 deoxyadenosine pH 8.0, 37°C Homo sapiens
2.7.1.74 0.15
-
 deoxyguanosine pH 8.0, 37°C Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.1.74 mitochondrion not Homo sapiens 5739
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.74 Mg2+ Mg2+/ATP complex is the reactive species Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.74 30000
-
 2 * 30000, SDS-PAGE Homo sapiens
2.7.1.74 61000
-
 gel filtration, glycerol gradient centrifugation Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.74 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.74 affinity chromatography Homo sapiens
2.7.1.74 spleen Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.1.74 leukemia cell leukemic T-lymphoblasts Homo sapiens
-
2.7.1.74 leukemia cell from spleen Homo sapiens
-
2.7.1.74 additional information very low activity in normal spleens and placentas Homo sapiens
-
2.7.1.74 spleen
-
 Homo sapiens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.74 0.11 0.15 pH 8.0, 37°C Homo sapiens

Storage Stability

EC Number Storage Stability Organism
2.7.1.74 -70°C, concentrated enzyme solution in Tris-buffer with MgCl2 and mercaptoethanol, at least 12 months Homo sapiens
2.7.1.74 4°C, concentrated enzyme solution in Tris-buffer with MgCl2 and mercaptoethanol, at least 2 weeks Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.74 ATP + 2'-deoxycytidine
-
 Homo sapiens ADP + 5'-dCMP
-
 ?
2.7.1.74 dTTP + 2'-deoxycytidine
-
 Homo sapiens dTDP + 2'-deoxy-CMP
-
 ?
2.7.1.74 additional information presumably one common nucleoside acceptor site Homo sapiens ?
-
 ?
2.7.1.74 NTP + cytosine arabinoside
-
 Homo sapiens NDP + cytosine arabinoside 5'-monophosphate
-
 ?
2.7.1.74 NTP + deoxyadenosine
-
 Homo sapiens NDP + dAMP
-
 ?
2.7.1.74 NTP + deoxycytidine
-
 Homo sapiens NDP + dCMP
-
 ?
2.7.1.74 NTP + deoxyguanosine
-
 Homo sapiens NDP + dGMP
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.1.74 dimer 2 * 30000, SDS-PAGE Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.1.74 37
-
 assay at Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.74 5.5 8.5 plateau, higher activity in Tris than in phosphate buffer Homo sapiens