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Literature summary extracted from

  • Datta, N.S.; Shewach, D.S.; Hurley, M.C.; Mitchell, B.S.; Fox, I.H.
    Human T-lymphoblast deoxycytidine kinase: purification and properties (1989), Biochemistry, 28, 114-123.
    View publication on PubMed

General Stability

EC Number General Stability Organism
2.7.1.74 dithiothreitol, MgCl2, ATP, KCl and glycerol stabilize Homo sapiens
2.7.1.74 very unstable at low protein concentrations Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.74 2-chloroadenosine
-
Homo sapiens
2.7.1.74 Ba2+
-
Homo sapiens
2.7.1.74 Cu2+
-
Homo sapiens
2.7.1.74 cytidine deoxycytidine as substrate Homo sapiens
2.7.1.74 cytosine arabinoside deoxycytidine as substrate Homo sapiens
2.7.1.74 deoxyadenosine deoxycytidine Homo sapiens
2.7.1.74 deoxyguanosine deoxycytidine as substrate Homo sapiens
2.7.1.74 Dideoxycytidine
-
Homo sapiens
2.7.1.74 HgCl2
-
Homo sapiens
2.7.1.74 additional information little or no inhibition by AMP, dTMP, adenosine, guanosine; little or no inhibition by deoxyuridine; little or no inhibition by ribavirin; no inhibition by inosine, xanthosine, S-adenosylhomocysteine, S-adenosylmethionine; no inhibition by uridine, thymidine; overview Homo sapiens
2.7.1.74 Nucleosides
-
Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.74 0.0008 0.0015 deoxycytidine 37°C, pH 7.0 Homo sapiens
2.7.1.74 0.02 0.04 cytosine arabinoside pH 7.0, 37°C Homo sapiens
2.7.1.74 0.33 0.43 deoxyguanosine 37°C, pH 7.0 Homo sapiens
2.7.1.74 0.45
-
cytidine 37°C, pH 7.0 Homo sapiens
2.7.1.74 0.5
-
deoxyadenosine 37°C, pH 7.0 Homo sapiens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.74 Ca2+ activation Homo sapiens
2.7.1.74 Co2+ activation Homo sapiens
2.7.1.74 Co2+ about 30% as efficient as Mg2+ Homo sapiens
2.7.1.74 Fe2+ activation Homo sapiens
2.7.1.74 Fe2+ about 60% as efficient as Mg2+ Homo sapiens
2.7.1.74 Mg2+
-
Homo sapiens
2.7.1.74 Mn2+ requirement Homo sapiens
2.7.1.74 Mn2+ about 90% as efficient as Mg2+ Homo sapiens
2.7.1.74 additional information no activation by Ba2+, Cu2+ Homo sapiens
2.7.1.74 additional information no activation by monovalent cations Homo sapiens
2.7.1.74 Ni2+ not Homo sapiens
2.7.1.74 Zn2+ activation Homo sapiens
2.7.1.74 Zn2+ about 30% as efficient as Mg2+ Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.74 30500
-
2 * 30500, SDS-PAGE Homo sapiens
2.7.1.74 60000
-
gel filtration Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.74 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.74 affinity chromatography Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.1.74 leukemia cell leukemic T-lymphoblasts Homo sapiens
-
2.7.1.74 leukemia cell cell line MOLT-4 Homo sapiens
-
2.7.1.74 MOLT-4 cell
-
Homo sapiens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.74 4
-
cytidine as substrate, 37°C, pH 7.0 Homo sapiens
2.7.1.74 7.2
-
deoxyguanosine as substrate, 37°C, pH 7.0 Homo sapiens
2.7.1.74 8
-
37°C, pH 7.0 Homo sapiens
2.7.1.74 13.5
-
deoxyadenosine as substrate, 37°C, pH 7.0 Homo sapiens

Storage Stability

EC Number Storage Stability Organism
2.7.1.74 -85°C, in 1 mg/ml bovine serum albumin, 20% v/v glycerol, 0.2 M potassium phosphate, 2 mM ATP, 2.4 mM MgCl2 and 0.025 M DTT, more than 12 months Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.74 ATP + 2'-deoxycytidine
-
Homo sapiens ADP + 5'-dCMP
-
?
2.7.1.74 ATP + cytidine
-
Homo sapiens ADP + ?
-
?
2.7.1.74 CTP + 2'-deoxycytidine poor Homo sapiens CDP + 2'-deoxy-CMP
-
?
2.7.1.74 dGTP + 2'-deoxycytidine best phosphate donor Homo sapiens dGDP + 2'-deoxy-CMP
-
?
2.7.1.74 GTP + 2'-deoxycytidine as good as ATP Homo sapiens GDP + 2'-deoxy-CMP
-
?
2.7.1.74 NTP + cytosine arabinoside
-
Homo sapiens NDP + cytosine arabinoside 5'-monophosphate
-
?
2.7.1.74 NTP + deoxyadenosine
-
Homo sapiens NDP + dAMP
-
?
2.7.1.74 NTP + deoxycytidine enzyme shows little specificity towards phosphate donor Homo sapiens NDP + dCMP
-
?
2.7.1.74 NTP + deoxycytidine no substrate: nucleoside diphosphates and their deoxy derivatives Homo sapiens NDP + dCMP
-
?
2.7.1.74 NTP + deoxyguanosine
-
Homo sapiens NDP + dGMP
-
?
2.7.1.74 UTP + 2'-deoxycytidine poor Homo sapiens UDP + 2'-deoxy-CMP
-
?

Subunits

EC Number Subunits Comment Organism
2.7.1.74 dimer 2 * 30500, SDS-PAGE Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.1.74 37
-
assay at Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.74 7
-
broad Homo sapiens

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.7.1.74 5.8 10 about half-maximal activity at pH 5.8 and pH 10, about 90% of maximal activity at pH 6.5 and pH 9 Homo sapiens