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Literature summary extracted from

  • Wang, L.M.; Kucera, G.L.; Capizzi, R.L.
    Purification and characterization of deoxycytidine kinase from acute myeloid leukemia cell mitochondria (1993), Biochim. Biophys. Acta, 1202, 309-316.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.1.74 deoxyadenosine activation, deoxycytidine as substrate, mitochondrial isozyme Homo sapiens
2.7.1.74 deoxyguanosine activation, deoxycytidine as substrate, mitochondrial isozyme Homo sapiens
2.7.1.74 TTP stimulation, cytosolic isozyme I Homo sapiens
2.7.1.74 UDP stimulation, mitochondrial isozyme Homo sapiens
2.7.1.74 Uridine arabinoside activation, deoxycytidine as substrate, mitochondrial isozyme Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.74 Cytosine arabinoside triphosphate strong Homo sapiens
2.7.1.74 dCMP only cytosolic isozyme I; product inhibition, strong Homo sapiens
2.7.1.74 dCTP cytosine arabinoside as substrate; isozyme II Homo sapiens
2.7.1.74 deoxyadenosine cytosolic isozyme I, weak Homo sapiens
2.7.1.74 deoxycytidine cytosine arabinoside as substrate Homo sapiens
2.7.1.74 deoxyguanosine deoxyguanosine as substrate, cytosolic isozyme I, weak Homo sapiens
2.7.1.74 deoxythymidine weak Homo sapiens
2.7.1.74 dGDP not Homo sapiens
2.7.1.74 dTTP only mitochondrial isozyme; strong Homo sapiens
2.7.1.74 dUDP only cytosolic isozyme I; strong Homo sapiens
2.7.1.74 GTP not, deoxyadenosine as substrate Homo sapiens
2.7.1.74 UDP only cytosolic isozyme I; strong Homo sapiens
2.7.1.74 Uridine arabinoside weak, cytosolic isozyme I Homo sapiens
2.7.1.74 UTP not mitochondrial isozyme Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.74 0.002
-
 cytosine arabinoside deoxycytidine, cytosolic isozyme I, pH 7.5, 37°C Homo sapiens
2.7.1.74 0.007
-
 cytosine arabinoside cytosolic isozyme I, pH 7.5, 37°C Homo sapiens
2.7.1.74 0.014 0.0167 deoxycytidine pH 7.5, 37°C Homo sapiens
2.7.1.74 0.014 0.0167 deoxycytidine mitochondrial isozyme, pH 7.5, 37°C Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.1.74 cytosol 2 isozymes: I and II Homo sapiens 5829
-
2.7.1.74 mitochondrion outer membrane Homo sapiens 5739
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.74 52000
-
 1 * 52000, SDS-PAGE, mitochondrial isozyme Homo sapiens
2.7.1.74 70000
-
 human cytoplasmic isozymes Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.74 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.74 affinity chromatography Homo sapiens
2.7.1.74 mitochondrial isozyme (extracted with digitonin) Homo sapiens
2.7.1.74 partial Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.1.74 BG-1 cell
-
 Homo sapiens
-
2.7.1.74 leukemia cell from peripheral blood Homo sapiens
-
2.7.1.74 leukemia cell from acute myeloid leukemia (AML) patients Homo sapiens
-
2.7.1.74 leukemia cell ML-1 cells Homo sapiens
-
2.7.1.74 ML-1 cell
-
 Homo sapiens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.74 0.048
-
 mitochondrial isozyme, pH 7.5, 37°C Homo sapiens
2.7.1.74 0.072
-
 cytoplasmic isozyme I, pH 7.5, 37°C Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.74 ATP + 2'-deoxycytidine best phosphate donor cytosolic isozyme II and mitochondrial isozyme Homo sapiens ADP + 5'-dCMP
-
 ?
2.7.1.74 CTP + 2'-deoxycytidine as good as GTP, UTP, dATP, mitochondrial isozyme Homo sapiens CDP + 2'-deoxy-CMP
-
 ?
2.7.1.74 dGTP + 2'-deoxycytidine cytosolic isozyme I Homo sapiens dGDP + 2'-deoxy-CMP
-
 ?
2.7.1.74 dGTP + 2'-deoxycytidine poor substrate of mitochondrial isozyme Homo sapiens dGDP + 2'-deoxy-CMP
-
 ?
2.7.1.74 dUTP + 2'-deoxycytidine best phosphate donor, cytosolic enzyme I, poor, mitochondrial isozyme Homo sapiens dUDP + 2'-deoxy-CMP
-
 ?
2.7.1.74 GTP + 2'-deoxycytidine as good as dGTP, dTTP or dUTP, cytosolic isozyme I Homo sapiens GDP + 2'-deoxy-CMP
-
 ?
2.7.1.74 GTP + 2'-deoxycytidine as good as CTP, dATP or UTP, mitochondrial isozyme Homo sapiens GDP + 2'-deoxy-CMP
-
 ?
2.7.1.74 additional information several isozymes: cytosolic deoxycytidine kinase I and II, plus mitochondrial isozyme Homo sapiens ?
-
 ?
2.7.1.74 NTP + 2',2'-difluoro-2'-deoxycytidine cytosolic isozyme I, not mitochondrial isozyme Homo sapiens NDP + 2',2'-difluoro-2'-deoxycytidine 5'-phosphate
-
 ?
2.7.1.74 NTP + cytosine arabinoside D-isomer Homo sapiens NDP + cytosine arabinoside 5'-monophosphate
-
 ?
2.7.1.74 NTP + cytosine arabinoside cytosolic isozyme I Homo sapiens NDP + cytosine arabinoside 5'-monophosphate
-
 ?
2.7.1.74 NTP + cytosine arabinoside no acceptor substrate for cytosolic isozyme II or mitochondrial isozyme Homo sapiens NDP + cytosine arabinoside 5'-monophosphate
-
 ?
2.7.1.74 NTP + deoxyadenosine cytosolic isozyme I Homo sapiens NDP + dAMP
-
 ?
2.7.1.74 NTP + deoxyadenosine not mitochondrial isozyme Homo sapiens NDP + dAMP
-
 ?
2.7.1.74 NTP + deoxycytidine no substrate: guanosine Homo sapiens NDP + dCMP
-
 ?
2.7.1.74 NTP + deoxycytidine poor substrate: arabinosyl-CTP Homo sapiens NDP + dCMP
-
 ?
2.7.1.74 NTP + deoxycytidine no substrate: uridine arabinoside, cytosine Homo sapiens NDP + dCMP
-
 ?
2.7.1.74 NTP + deoxycytidine no substrate: adenosine, uridine Homo sapiens NDP + dCMP
-
 ?
2.7.1.74 NTP + deoxyguanosine cytosolic isozyme I Homo sapiens NDP + dGMP
-
 ?
2.7.1.74 NTP + deoxyguanosine not mitochondrial isozyme Homo sapiens NDP + dGMP
-
 ?
2.7.1.74 NTP + deoxythymidine mitochondrial isozyme, better than deoxycytidine, not cytosolic isozyme I Homo sapiens NDP + dTMP
-
 ?
2.7.1.74 NTP + deoxyuridine mitochondrial isozyme, not cytosolic isozyme I Homo sapiens NDP + dUMP
-
 ?
2.7.1.74 UTP + 2'-deoxycytidine as good as CTP, GTP or dATP Homo sapiens UDP + 2'-deoxy-CMP
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.1.74 monomer 1 * 52000, SDS-PAGE, mitochondrial isozyme Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.1.74 37
-
 assay at Homo sapiens