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Literature summary extracted from

  • Kierdaszuk, B.; Eriksson, S.
    Selective inactivation of the deoxyadenosine phosphorylating activity of pure human deoxycytidine kinase: stabilization of different forms of the enzyme by substrates and biological detergents (1990), Biochemistry, 29, 4109-4114.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.1.74 CHAPS slight stimulation, deoxycytidine as substrate Homo sapiens
2.7.1.74 sodium cholate slight stimulation, deoxycytidine as substrate Homo sapiens
2.7.1.74 Zwittergent 3-14 slight stimulation, deoxycytidine as substrate Homo sapiens

General Stability

EC Number General Stability Organism
2.7.1.74 bovine serum albumin stabilizes Homo sapiens
2.7.1.74 dTTP, ATP or deoxycytidine stabilizes Homo sapiens
2.7.1.76 stability of deoxyadenosine kinase activity differs appreciably from deoxycytidine kinase activity Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.74 additional information deoxycytidine phosphorylating ability shows different inhibition pattern than deoxyadenosine activity Homo sapiens
2.7.1.76 CHAPS at critical micelle concentration Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.74 additional information
-
 additional information
-
 Homo sapiens
2.7.1.76 additional information
-
 additional information kinetic study Homo sapiens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.74 Mg2+
-
 Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.74 ATP + deoxycytidine Homo sapiens key anabolic enzyme for activation of purine or pyrimidine deoxynucleosides as well as cytidine arabinoside and other anti-tumor drugs ADP + ?
-
 ?
2.7.1.76 ATP + deoxyadenosine Homo sapiens key anabolic enzyme for activation of purine and pyrimidine deoxyribonucleosides as well as cytosine arabinoside and other anti-tumour drugs ADP + 5'-dAMP
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.74 Homo sapiens
-
-
-
2.7.1.76 Homo sapiens
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.1.74 leukemia cell from spleen Homo sapiens
-
2.7.1.74 spleen
-
 Homo sapiens
-
2.7.1.76 spleen leukemic Homo sapiens
-

Storage Stability

EC Number Storage Stability Organism
2.7.1.74 4°C, t1/2: 7 days with and t1/2: 72 h without bovine serum albumin Homo sapiens
2.7.1.76 4°C, t1/2: 84 h with and t1/2: 32 h without addition of bovine serum albumin Homo sapiens
2.7.1.76 ATP, dTTP, deoxyadenosine or CHAPS stabilizes at 4°C Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.74 ATP + 2'-deoxycytidine
-
 Homo sapiens ADP + 5'-dCMP
-
 ?
2.7.1.74 ATP + deoxycytidine key anabolic enzyme for activation of purine or pyrimidine deoxynucleosides as well as cytidine arabinoside and other anti-tumor drugs Homo sapiens ADP + ?
-
 ?
2.7.1.74 additional information multisubstrate enzyme, that also phosphorylates purine deoxyribonucleotides Homo sapiens ?
-
 ?
2.7.1.74 additional information enzyme exists in different conformational states with different substrate kinetic properties Homo sapiens ?
-
 ?
2.7.1.74 NTP + deoxyadenosine
-
 Homo sapiens NDP + dAMP
-
 ?
2.7.1.74 NTP + deoxycytidine
-
 Homo sapiens NDP + dCMP
-
 ?
2.7.1.76 ATP + deoxyadenosine
-
 Homo sapiens ADP + dAMP
-
 ?
2.7.1.76 ATP + deoxyadenosine key anabolic enzyme for activation of purine and pyrimidine deoxyribonucleosides as well as cytosine arabinoside and other anti-tumour drugs Homo sapiens ADP + 5'-dAMP
-
 ?
2.7.1.76 ATP + deoxycytidine deoxycytidine kinase is a multisubstrate enzyme that also phosphorylates deoxyadenosine, it exists in different conformational states with different substrate kinetic properties Homo sapiens ADP + dCMP
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.1.74 50
-
-
 Homo sapiens
2.7.1.76 44
-
-
 Homo sapiens

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.7.1.74 37 55 about half-maximal activity at 37°C and 55°C Homo sapiens
2.7.1.76 27 50 approx. half-maximal activity at 27°C and 50°C Homo sapiens

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.7.1.74 additional information
-
 temperature stability of deoxycytidine kinase activity differs appreciably from deoxyadenosine kinase activity Homo sapiens
2.7.1.74 37
-
 at least 6 h stable Homo sapiens
2.7.1.74 54
-
 inactivation Homo sapiens
2.7.1.76 additional information
-
 temperature stability of deoxyadenosine kinase activity differs appreciably from deoxycytidine kinase activity Homo sapiens
2.7.1.76 4
-
 half-life of deoxyadenosine kinase activity: 1.5 days Homo sapiens
2.7.1.76 37
-
 t1/2: 2 h, 1 mM ATP, 0.5 mM dTTP, 1 mM deoxyadenosine or 0.25 3-[(3-cholamidopropyl)dimethylamino]-1-propanesulfonate stabilize Homo sapiens
2.7.1.76 48
-
 inactivation of deoxyadenosine kinase activity Homo sapiens
2.7.1.76 50
-
 80% loss of activity Homo sapiens