BRENDA - Enzyme Database

Deoxycytidine kinase from calf thymus. Substrate and inhibitor specificity

Krenitsky, T.A.; Tuttle, J.V.; Koszalka, G.W.; Chen, I.S.; Beacham, L.M.; Rideout, J.L.; Elion, G.B.; J. Biol. Chem. 251, 4055-4061 (1976)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
2.7.1.74
alpha-D-2'-Deoxythioguanosine
-
Bos taurus
2.7.1.74
cytidine
alpha-isomer
Bos taurus
2.7.1.74
additional information
no inhibition by ribavirin
Bos taurus
2.7.1.74
N6-Phenyladenosine
-
Bos taurus
2.7.1.74
Non-substrate inhibitors
-
Bos taurus
2.7.1.74
Substrate derivatives
overview
Bos taurus
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.7.1.74
additional information
-
additional information
Km-values of a variety of substrate derivatives
Bos taurus
2.7.1.74
0.002
-
cytosine arabinoside
L-isomer, 25C, pH 7.6
Bos taurus
2.7.1.74
0.02
0.04
cytosine arabinoside
D-isomer; pH 7.6, 25C
Bos taurus
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
2.7.1.74
Mg2+
-
Bos taurus
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.7.1.74
Bos taurus
-
-
-
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
2.7.1.74
thymus
-
Bos taurus
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.1.74
ATP + 2'-deoxycytidine
-
641935
Bos taurus
ADP + 5'-dCMP
-
641935
Bos taurus
?
2.7.1.74
ATP + cytidine
poor: beta-isomer
641935
Bos taurus
ADP + ?
-
-
-
?
2.7.1.74
ATP + cytidine
not: alpha-isomer
641935
Bos taurus
ADP + ?
-
-
-
?
2.7.1.74
NTP + beta-D-2'-deoxythioguanosine
not alpha-isomer
641935
Bos taurus
NDP + ?
-
-
-
?
2.7.1.74
NTP + cytosine arabinoside
i.e. 1-beta-arabinofuranosyl cytosine, L-isomer better substrate than D-isomer
641935
Bos taurus
NDP + cytosine arabinoside 5'-monophosphate
-
-
-
?
2.7.1.74
NTP + deoxycytidine
specificity
641935
Bos taurus
NDP + dCMP
-
641935
Bos taurus
?
2.7.1.74
NTP + deoxycytidine
no substrate: ribavirin
641935
Bos taurus
NDP + dCMP
-
641935
Bos taurus
?
2.7.1.74
NTP + deoxycytidine
beta-D-2'-deoxyribonucleosides are more efficient than corresponding beta-D-arabinonucleosides or beta-ribonucleosides, rather nonspecific for base moiety of nucleoside substrate
641935
Bos taurus
NDP + dCMP
-
641935
Bos taurus
?
2.7.1.74
NTP + deoxycytidine
no substrate: adenosine, uridine
641935
Bos taurus
NDP + dCMP
-
641935
Bos taurus
?
2.7.1.74
NTP + deoxycytidine
poor substrate: beta-5-azacytidine
641935
Bos taurus
NDP + dCMP
-
641935
Bos taurus
?
2.7.1.74
NTP + deoxyguanosine
-
641935
Bos taurus
NDP + dGMP
-
-
-
?
Temperature Optimum [C]
EC Number
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
2.7.1.74
25
-
assay at
Bos taurus
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
2.7.1.74
alpha-D-2'-Deoxythioguanosine
-
Bos taurus
2.7.1.74
cytidine
alpha-isomer
Bos taurus
2.7.1.74
additional information
no inhibition by ribavirin
Bos taurus
2.7.1.74
N6-Phenyladenosine
-
Bos taurus
2.7.1.74
Non-substrate inhibitors
-
Bos taurus
2.7.1.74
Substrate derivatives
overview
Bos taurus
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.7.1.74
additional information
-
additional information
Km-values of a variety of substrate derivatives
Bos taurus
2.7.1.74
0.002
-
cytosine arabinoside
L-isomer, 25C, pH 7.6
Bos taurus
2.7.1.74
0.02
0.04
cytosine arabinoside
D-isomer; pH 7.6, 25C
Bos taurus
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
2.7.1.74
Mg2+
-
Bos taurus
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
2.7.1.74
thymus
-
Bos taurus
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.1.74
ATP + 2'-deoxycytidine
-
641935
Bos taurus
ADP + 5'-dCMP
-
641935
Bos taurus
?
2.7.1.74
ATP + cytidine
poor: beta-isomer
641935
Bos taurus
ADP + ?
-
-
-
?
2.7.1.74
ATP + cytidine
not: alpha-isomer
641935
Bos taurus
ADP + ?
-
-
-
?
2.7.1.74
NTP + beta-D-2'-deoxythioguanosine
not alpha-isomer
641935
Bos taurus
NDP + ?
-
-
-
?
2.7.1.74
NTP + cytosine arabinoside
i.e. 1-beta-arabinofuranosyl cytosine, L-isomer better substrate than D-isomer
641935
Bos taurus
NDP + cytosine arabinoside 5'-monophosphate
-
-
-
?
2.7.1.74
NTP + deoxycytidine
specificity
641935
Bos taurus
NDP + dCMP
-
641935
Bos taurus
?
2.7.1.74
NTP + deoxycytidine
no substrate: ribavirin
641935
Bos taurus
NDP + dCMP
-
641935
Bos taurus
?
2.7.1.74
NTP + deoxycytidine
beta-D-2'-deoxyribonucleosides are more efficient than corresponding beta-D-arabinonucleosides or beta-ribonucleosides, rather nonspecific for base moiety of nucleoside substrate
641935
Bos taurus
NDP + dCMP
-
641935
Bos taurus
?
2.7.1.74
NTP + deoxycytidine
no substrate: adenosine, uridine
641935
Bos taurus
NDP + dCMP
-
641935
Bos taurus
?
2.7.1.74
NTP + deoxycytidine
poor substrate: beta-5-azacytidine
641935
Bos taurus
NDP + dCMP
-
641935
Bos taurus
?
2.7.1.74
NTP + deoxyguanosine
-
641935
Bos taurus
NDP + dGMP
-
-
-
?
Temperature Optimum [C] (protein specific)
EC Number
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
2.7.1.74
25
-
assay at
Bos taurus