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Literature summary extracted from

  • Kozai, Y.; Sonoda, S.; Kobayashi, S.; Sugino, Y.
    Metabolism in deoxyribonucleotides. IV. Purification and properties of deoxycytidine kinase of calf thymus (1972), J. Biochem., 71, 485-496.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.74 ADP
-
 Bos taurus
2.7.1.74 CDP strong Bos taurus
2.7.1.74 CTP
-
 Bos taurus
2.7.1.74 cytidine not Bos taurus
2.7.1.74 dADP strong Bos taurus
2.7.1.74 dATP strong Bos taurus
2.7.1.74 dCDP strong, ATP as substrate Bos taurus
2.7.1.74 dCMP ATP as substrate; product inhibition, strong Bos taurus
2.7.1.74 dCTP ATP as substrate Bos taurus
2.7.1.74 deoxycytidine cytidine as substrate; deoxyguanosine as substrate Bos taurus
2.7.1.74 deoxyguanosine not Bos taurus
2.7.1.74 dGDP strong Bos taurus
2.7.1.74 dGTP
-
 Bos taurus
2.7.1.74 dTDP strong Bos taurus
2.7.1.74 dTTP
-
 Bos taurus
2.7.1.74 dUDP strong Bos taurus
2.7.1.74 GDP
-
 Bos taurus
2.7.1.74 GTP
-
 Bos taurus
2.7.1.74 Tris-maleate buffer
-
 Bos taurus
2.7.1.74 UDP strong Bos taurus
2.7.1.74 UTP
-
 Bos taurus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.74 additional information
-
 additional information kinetic study Bos taurus
2.7.1.74 additional information
-
 additional information linear kinetics with deoxyguanosine or cytidine, bimodal kinetics with deoxycytidine as substrate Bos taurus
2.7.1.74 1.4
-
 cytidine 37°C, pH 8.0 Bos taurus
2.7.1.74 3
-
 deoxyguanosine 37°C, pH 8.0 Bos taurus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.1.74 cytosol
-
 Bos taurus 5829
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.74 Ca2+ activation Bos taurus
2.7.1.74 Mg2+ below: decrease of activity Bos taurus
2.7.1.74 Mg2+ Mg2+/ATP complex is the reactive species Bos taurus
2.7.1.74 Mg2+ maximal activity at a ATP:Mg2+-ratio of 1:1 Bos taurus
2.7.1.74 Mn2+ requirement Bos taurus
2.7.1.74 Ni2+ activation Bos taurus
2.7.1.74 Zn2+ activation Bos taurus

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.74 Bos taurus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.74 partial Bos taurus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.1.74 thymus
-
 Bos taurus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.74 0.00523
-
 37°C, pH 8.0 Bos taurus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.74 ATP + 2'-deoxycytidine
-
 Bos taurus ADP + 5'-dCMP
-
 ?
2.7.1.74 ATP + cytidine
-
 Bos taurus ADP + ?
-
 ?
2.7.1.74 CTP + 2'-deoxycytidine
-
 Bos taurus CDP + 2'-deoxy-CMP
-
 ?
2.7.1.74 dGTP + 2'-deoxycytidine best phosphate donor Bos taurus dGDP + 2'-deoxy-CMP
-
 ?
2.7.1.74 dTTP + 2'-deoxycytidine
-
 Bos taurus dTDP + 2'-deoxy-CMP
-
 ?
2.7.1.74 GTP + 2'-deoxycytidine
-
 Bos taurus GDP + 2'-deoxy-CMP
-
 ?
2.7.1.74 NTP + deoxycytidine no substrate: dCTP Bos taurus NDP + dCMP
-
 ?
2.7.1.74 NTP + deoxycytidine no substrate: guanosine Bos taurus NDP + dCMP
-
 ?
2.7.1.74 NTP + deoxycytidine in order of decreasing activity: dGTP, CTP, ATP, dATP, dTTP, UTP, GTP Bos taurus NDP + dCMP
-
 ?
2.7.1.74 NTP + deoxycytidine no substrate: adenosine, uridine Bos taurus NDP + dCMP
-
 ?
2.7.1.74 NTP + deoxycytidine no substrate: thymidine Bos taurus NDP + dCMP
-
 ?
2.7.1.74 NTP + deoxyguanosine
-
 Bos taurus NDP + dGMP
-
 ?
2.7.1.74 UTP + 2'-deoxycytidine
-
 Bos taurus UDP + 2'-deoxy-CMP
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.1.74 37
-
 assay at Bos taurus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.74 8
-
-
 Bos taurus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.7.1.74 7 10 about 80% of maximal activity at pH 7 and about 65% of maximal activity at pH 10 Bos taurus