Literature summary extracted from

Meyers, M.B.; Kreis, W.
Purification of deoxycytidine kinases from two P815 murine neoplasms and their separation from deoxyguanosine kinase (1976), Arch. Biochem. Biophys., 177, 10-15.

Activating Compound

EC Number	Activating Compound	Comment	Organism	Structure
2.7.1.74	dithiothreitol	activation	Mus musculus

General Stability

EC Number	General Stability	Organism
2.7.1.74	dithiothreitol stabilizes	Mus musculus

KM Value [mM]

EC Number	KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
2.7.1.74	0.0033	0.0093	deoxycytidine	37°C, pH 8.0	Mus musculus
2.7.1.74	0.014	0.0167	deoxycytidine	37°C, pH 8.0	Mus musculus
2.7.1.74	0.014	0.0167	deoxycytidine	cytosine arabinoside-induced cells, 37°C, pH 8.0	Mus musculus

Localization

EC Number	Localization	Comment	Organism	GeneOntology No.	Textmining
2.7.1.74	cytosol	-	Mus musculus	5829	-

Metals/Ions

EC Number	Metals/Ions	Comment	Organism	Structure
2.7.1.74	Mg2+	-	Mus musculus

Organism

EC Number	Organism	UniProt	Comment	Textmining
2.7.1.74	Mus musculus	-	-	-

Purification (Commentary)

EC Number	Purification (Comment)	Organism
2.7.1.74	-	Mus musculus

Source Tissue

EC Number	Source Tissue	Comment	Organism	Textmining
2.7.1.74	carcinoma cell line	P815-neoplasm cell line	Mus musculus	-
2.7.1.74	P-815 cell	-	Mus musculus	-

Specific Activity [micromol/min/mg]

EC Number	Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
2.7.1.74	0.00088	-	cytosine arabinoside-induced cells	Mus musculus
2.7.1.74	0.0846	-	37°C, pH 8.0	Mus musculus

Substrates and Products (Substrate)

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
2.7.1.74	ATP + 2'-deoxycytidine	-	Mus musculus	ADP + 5'-dCMP	-	?
2.7.1.74	NTP + deoxycytidine	no substrate: deoxyuridine	Mus musculus	NDP + dCMP	-	?

Temperature Optimum [°C]

EC Number	Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
2.7.1.74	37	-	assay at	Mus musculus

Turnover Number [1/s]

EC Number	Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
2.7.1.74	1330	-	deoxycytidine	cytosine arabinoside-induced cells	Mus musculus
2.7.1.74	78300	-	deoxycytidine	-	Mus musculus

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Release 2023.1 (January 2023)