BRENDA - Enzyme Database

Properties of deoxycytidine kinase partially purified from L1210 cells

Kessel, D.; J. Biol. Chem. 243, 4739-4744 (1968)

Data extracted from this reference:

General Stability
EC Number
General Stability
Organism
2.7.1.74
bovine serum albumin does not stabilize
Mus musculus
2.7.1.74
protamine treatment, MnCl2-treatment or ammonium sulfate fractionation inactivates during purification
Mus musculus
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
2.7.1.74
cytidine
not
Mus musculus
2.7.1.74
cytosine arabinoside
deoxycytidine as substrate
Mus musculus
2.7.1.74
dAMP
not
Mus musculus
2.7.1.74
dCDP
-
Mus musculus
2.7.1.74
dCMP
-
Mus musculus
2.7.1.74
dCTP
strong, pH-dependent
Mus musculus
2.7.1.74
dGMP
not
Mus musculus
2.7.1.74
dinitrophenol
weak
Mus musculus
2.7.1.74
dioxane
-
Mus musculus
2.7.1.74
dUMP
not
Mus musculus
2.7.1.74
HgCl2
-
Mus musculus
2.7.1.74
IAA
weak
Mus musculus
2.7.1.74
additional information
little or no inhibition by deoxyuridine; no inhibition by TTP; no inhibition by uridine, thymidine; relatively insensitive to SH-inhibitors and detergents
Mus musculus
2.7.1.74
N-ethylmaleimide
weak
Mus musculus
2.7.1.74
SDS
-
Mus musculus
2.7.1.74
Urea
-
Mus musculus
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.7.1.74
additional information
-
additional information
kinetic study
Mus musculus
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.7.1.74
cytosol
-
Mus musculus
5829
-
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
2.7.1.74
Ca2+
activation; can replace Mn2+ or Mg2+ to some extent
Mus musculus
2.7.1.74
Fe2+
activation; can replace Mn2+ or Mg2+ to some extent
Mus musculus
2.7.1.74
Mg2+
requirement, 2 mM
Mus musculus
2.7.1.74
Mn2+
0.5 mM; requirement
Mus musculus
2.7.1.74
additional information
no activation by Ba2+, Cu2+
Mus musculus
2.7.1.74
Zn2+
not
Mus musculus
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.7.1.74
60000
-
gel filtration
Mus musculus
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.7.1.74
Mus musculus
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
2.7.1.74
partial
Mus musculus
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
2.7.1.74
leukemia cell
L1210
Mus musculus
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
2.7.1.74
0.0048
-
37C, pH 7.0
Mus musculus
Storage Stability
EC Number
Storage Stability
Organism
2.7.1.74
-20C, 3 months
Mus musculus
2.7.1.74
4C, at least 1 week
Mus musculus
2.7.1.74
glycerol, bovine serum albumin, 2-mercaptoethanol or EDTA does not enhance storage stability
Mus musculus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.1.74
ATP + 2'-deoxycytidine
best phosphate donor
641929
Mus musculus
ADP + 5'-dCMP
-
641929
Mus musculus
?
2.7.1.74
NTP + cytosine arabinoside
-
641929
Mus musculus
NDP + cytosine arabinoside 5'-monophosphate
-
-
-
?
2.7.1.74
NTP + deoxycytidine
no substrate: phosphate
641929
Mus musculus
NDP + dCMP
-
641929
Mus musculus
?
2.7.1.74
NTP + deoxycytidine
enzyme shows little specificity towards phosphate donor
641929
Mus musculus
NDP + dCMP
-
641929
Mus musculus
?
2.7.1.74
UTP + 2'-deoxycytidine
best phosphate donor at low substrate levels
641929
Mus musculus
UDP + 2'-deoxy-CMP
-
-
-
?
2.7.1.74
UTP + 2'-deoxycytidine
as good as CTP, GTP or dATP
641929
Mus musculus
UDP + 2'-deoxy-CMP
-
-
-
?
Temperature Optimum [C]
EC Number
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
2.7.1.74
37
-
assay at
Mus musculus
Temperature Range [C]
EC Number
Temperature Minimum [C]
Temperature Maximum [C]
Commentary
Organism
2.7.1.74
27
37
moderately sensitive to variations of incubation temperature
Mus musculus
Temperature Stability [C]
EC Number
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
2.7.1.74
50
-
10 min, 10% loss of activity
Mus musculus
2.7.1.74
60
-
10 min, about 65% loss of activity
Mus musculus
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.7.1.74
5
7
broad
Mus musculus
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
2.7.1.74
5
10
maximal activity at pH 5 and about half-maximal activity at pH 10
Mus musculus
General Stability (protein specific)
EC Number
General Stability
Organism
2.7.1.74
bovine serum albumin does not stabilize
Mus musculus
2.7.1.74
protamine treatment, MnCl2-treatment or ammonium sulfate fractionation inactivates during purification
Mus musculus
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
2.7.1.74
cytidine
not
Mus musculus
2.7.1.74
cytosine arabinoside
deoxycytidine as substrate
Mus musculus
2.7.1.74
dAMP
not
Mus musculus
2.7.1.74
dCDP
-
Mus musculus
2.7.1.74
dCMP
-
Mus musculus
2.7.1.74
dCTP
strong, pH-dependent
Mus musculus
2.7.1.74
dGMP
not
Mus musculus
2.7.1.74
dinitrophenol
weak
Mus musculus
2.7.1.74
dioxane
-
Mus musculus
2.7.1.74
dUMP
not
Mus musculus
2.7.1.74
HgCl2
-
Mus musculus
2.7.1.74
IAA
weak
Mus musculus
2.7.1.74
additional information
little or no inhibition by deoxyuridine; no inhibition by TTP; no inhibition by uridine, thymidine; relatively insensitive to SH-inhibitors and detergents
Mus musculus
2.7.1.74
N-ethylmaleimide
weak
Mus musculus
2.7.1.74
SDS
-
Mus musculus
2.7.1.74
Urea
-
Mus musculus
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.7.1.74
additional information
-
additional information
kinetic study
Mus musculus
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.7.1.74
cytosol
-
Mus musculus
5829
-
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
2.7.1.74
Ca2+
activation; can replace Mn2+ or Mg2+ to some extent
Mus musculus
2.7.1.74
Fe2+
activation; can replace Mn2+ or Mg2+ to some extent
Mus musculus
2.7.1.74
Mg2+
requirement, 2 mM
Mus musculus
2.7.1.74
Mn2+
0.5 mM; requirement
Mus musculus
2.7.1.74
additional information
no activation by Ba2+, Cu2+
Mus musculus
2.7.1.74
Zn2+
not
Mus musculus
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.7.1.74
60000
-
gel filtration
Mus musculus
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.7.1.74
partial
Mus musculus
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
2.7.1.74
leukemia cell
L1210
Mus musculus
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
2.7.1.74
0.0048
-
37C, pH 7.0
Mus musculus
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
2.7.1.74
-20C, 3 months
Mus musculus
2.7.1.74
4C, at least 1 week
Mus musculus
2.7.1.74
glycerol, bovine serum albumin, 2-mercaptoethanol or EDTA does not enhance storage stability
Mus musculus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.1.74
ATP + 2'-deoxycytidine
best phosphate donor
641929
Mus musculus
ADP + 5'-dCMP
-
641929
Mus musculus
?
2.7.1.74
NTP + cytosine arabinoside
-
641929
Mus musculus
NDP + cytosine arabinoside 5'-monophosphate
-
-
-
?
2.7.1.74
NTP + deoxycytidine
no substrate: phosphate
641929
Mus musculus
NDP + dCMP
-
641929
Mus musculus
?
2.7.1.74
NTP + deoxycytidine
enzyme shows little specificity towards phosphate donor
641929
Mus musculus
NDP + dCMP
-
641929
Mus musculus
?
2.7.1.74
UTP + 2'-deoxycytidine
best phosphate donor at low substrate levels
641929
Mus musculus
UDP + 2'-deoxy-CMP
-
-
-
?
2.7.1.74
UTP + 2'-deoxycytidine
as good as CTP, GTP or dATP
641929
Mus musculus
UDP + 2'-deoxy-CMP
-
-
-
?
Temperature Optimum [C] (protein specific)
EC Number
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
2.7.1.74
37
-
assay at
Mus musculus
Temperature Range [C] (protein specific)
EC Number
Temperature Minimum [C]
Temperature Maximum [C]
Commentary
Organism
2.7.1.74
27
37
moderately sensitive to variations of incubation temperature
Mus musculus
Temperature Stability [C] (protein specific)
EC Number
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
2.7.1.74
50
-
10 min, 10% loss of activity
Mus musculus
2.7.1.74
60
-
10 min, about 65% loss of activity
Mus musculus
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.7.1.74
5
7
broad
Mus musculus
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
2.7.1.74
5
10
maximal activity at pH 5 and about half-maximal activity at pH 10
Mus musculus