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Literature summary extracted from

  • Sugiyama, M.; Sakamoto, M.; Mochizuki, H.; Nimi, O.; Nomi, R.
    Purification and characterization of streptomycin 6-kinase, an enzyme implicated in self-protection of a streptomycin-producing micro-organism (1983), J. Gen. Microbiol., 129, 1683-1687.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.72 adenosine competitive Streptomyces griseus
2.7.1.72 ADP noncompetitive Streptomyces griseus
2.7.1.72 AgNO3 strong inhibition Streptomyces griseus
2.7.1.72 AMP noncompetitive Streptomyces griseus
2.7.1.72 ATP
-
 Streptomyces griseus
2.7.1.72 diphosphate noncompetitive Streptomyces griseus
2.7.1.72 EDTA strong inhibition Streptomyces griseus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.72 0.4
-
 ATP
-
 Streptomyces griseus
2.7.1.72 3.5
-
 streptomycin
-
 Streptomyces griseus
2.7.1.72 11
-
 dihydrostreptomycin
-
 Streptomyces griseus
2.7.1.72 28
-
 streptidine
-
 Streptomyces griseus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.72 Co2+ 10 mM, rather less stimulatory than Mg2+ Streptomyces griseus
2.7.1.72 Cu2+ 10 mM, rather less stimulatory than Mg2+ Streptomyces griseus
2.7.1.72 Fe2+ 10 mM, slight stimulation Streptomyces griseus
2.7.1.72 Mg2+ required Streptomyces griseus
2.7.1.72 Mg2+ maximal activity with 10 mM Streptomyces griseus
2.7.1.72 Mn2+ 10 mM, slight stimulation Streptomyces griseus
2.7.1.72 additional information not affected by Ca2+, 0.2-100 mM Streptomyces griseus
2.7.1.72 Zn2+ 10 mM, slight stimulation Streptomyces griseus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.72 36000
-
 1 * 36000, SDS-PAGE Streptomyces griseus
2.7.1.72 38000
-
 gel filtration Streptomyces griseus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.72 ATP + streptomycin Streptomyces griseus self-protection of streptomycin producer, because the product streptomycin 6-phosphate does not inhibit protein synthesis ADP + streptomycin 6-phosphate
-
 ?
2.7.1.72 ATP + streptomycin Streptomyces griseus HUT 6037 self-protection of streptomycin producer, because the product streptomycin 6-phosphate does not inhibit protein synthesis ADP + streptomycin 6-phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.72 Streptomyces griseus
-
-
-
2.7.1.72 Streptomyces griseus HUT 6037
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.72
-
 Streptomyces griseus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.72 11.35
-
 purified enzyme Streptomyces griseus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.72 ATP + dihydrostreptomycin
-
 Streptomyces griseus ADP + dihydrostreptomycin 6-phosphate
-
 ?
2.7.1.72 ATP + dihydrostreptomycin
-
 Streptomyces griseus HUT 6037 ADP + dihydrostreptomycin 6-phosphate
-
 ?
2.7.1.72 ATP + streptomycin
-
 Streptomyces griseus ADP + streptomycin 6-phosphate
-
 ?
2.7.1.72 ATP + streptomycin self-protection of streptomycin producer, because the product streptomycin 6-phosphate does not inhibit protein synthesis Streptomyces griseus ADP + streptomycin 6-phosphate
-
 ?
2.7.1.72 ATP + streptomycin
-
 Streptomyces griseus HUT 6037 ADP + streptomycin 6-phosphate
-
 ?
2.7.1.72 ATP + streptomycin self-protection of streptomycin producer, because the product streptomycin 6-phosphate does not inhibit protein synthesis Streptomyces griseus HUT 6037 ADP + streptomycin 6-phosphate
-
 ?
2.7.1.72 additional information no activity with GTP, CTP, UTP Streptomyces griseus ?
-
 ?
2.7.1.72 additional information no activity with GTP, CTP, UTP Streptomyces griseus HUT 6037 ?
-
 ?
2.7.1.72 streptidine + ATP
-
 Streptomyces griseus streptidine 6-phosphate + ADP
-
 ?
2.7.1.72 streptidine + ATP
-
 Streptomyces griseus HUT 6037 streptidine 6-phosphate + ADP
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.1.72 monomer 1 * 36000, SDS-PAGE Streptomyces griseus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.72 additional information
-
 pI: 6.6 Streptomyces griseus

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.1.72 ATP
-
 Streptomyces griseus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.7.1.72 0.8
-
 adenosine
-
 Streptomyces griseus
2.7.1.72 3
-
 diphosphate
-
 Streptomyces griseus
2.7.1.72 5
-
 ADP
-
 Streptomyces griseus
2.7.1.72 6
-
 ATP
-
 Streptomyces griseus