EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.7.1.7 | D-fructose | competitive to glucose | Escherichia coli | |
2.7.1.7 | D-mannosamine | competitive | Escherichia coli | |
2.7.1.7 | D-mannose | competitive to glucose | Escherichia coli | |
2.7.1.7 | D-mannose 6-phosphate | up to 20 mM no effect | Escherichia coli | |
2.7.1.7 | L-sorbose | competitive | Escherichia coli | |
2.7.1.7 | lyxose | competitive | Escherichia coli | |
2.7.1.7 | MgADP- | - |
Escherichia coli | |
2.7.1.7 | Mn2+ | at a Mn2+/ATP ratio of more than 2 | Escherichia coli | |
2.7.1.7 | additional information | up to 20 mM fructose 6-phosphate shows no inhibitory effect | Escherichia coli |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.7.1.7 | 0.2 | - |
ATP | reaction with D-mannose | Escherichia coli | |
2.7.1.7 | 0.2 | - |
ATP | ATP in form of MgATP2- | Escherichia coli | |
2.7.1.7 | 0.3 | - |
D-fructose | pH 7.5, 25°C | Escherichia coli | |
2.7.1.7 | 0.3 | - |
ITP | pH 7.5, 25°C | Escherichia coli | |
2.7.1.7 | 0.5 | - |
GTP | pH 7.5, 25°C | Escherichia coli | |
2.7.1.7 | 0.6 | - |
dATP | pH 7.5, 25°C | Escherichia coli | |
2.7.1.7 | 2 | - |
CTP | pH 7.5, 25°C | Escherichia coli | |
2.7.1.7 | 2 | - |
UTP | pH 7.5, 25°C | Escherichia coli | |
2.7.1.7 | 4 | - |
D-glucose | pH 7.5, 25°C | Escherichia coli | |
2.7.1.7 | 4 | - |
TTP | pH 7.5, 25°C | Escherichia coli | |
2.7.1.7 | 5 | - |
D-glucosamine | pH 7.5, 25°C | Escherichia coli | |
2.7.1.7 | 10 | - |
D-glucose | pH 7.5, 25°C | Escherichia coli | |
2.7.1.7 | 10 | - |
2-deoxyglucose | pH 7.5, 25°C | Escherichia coli |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
2.7.1.7 | Co2+ | can partially replace Mg2+ | Escherichia coli | |
2.7.1.7 | Mg2+ | required | Escherichia coli | |
2.7.1.7 | Mn2+ | can partially replace Mg2+ | Escherichia coli | |
2.7.1.7 | additional information | no activation with Ca2+, Sn2+, Cd2+ | Escherichia coli | |
2.7.1.7 | Ni2+ | 40% of the activity with Mg2+ | Escherichia coli |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.7.1.7 | Escherichia coli | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.7.1.7 | partial | Escherichia coli |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
2.7.1.7 | 1.5 | - |
- |
Escherichia coli |
EC Number | Storage Stability | Organism |
---|---|---|
2.7.1.7 | -10°C, stable for several months | Escherichia coli |
2.7.1.7 | 4°C, stable for several days | Escherichia coli |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.7.1.7 | ATP + 2-deoxy-D-glucose | 20% of the activity with D-mannose | Escherichia coli | ADP + 2-deoxy-D-glucose 6-phosphate | - |
? | |
2.7.1.7 | ATP + 2-deoxy-D-mannose | no activity | Escherichia coli | ADP + 2-deoxy-D-mannose 6-phosphate | - |
? | |
2.7.1.7 | ATP + D-fructose | D-mannose and D-fructose phosphorylated equally well | Escherichia coli | ADP + D-fructose 6-phosphate | - |
? | |
2.7.1.7 | ATP + D-glucosamine | 5% of the activity with D-mannose | Escherichia coli | ADP + D-glucosamine 6-phosphate | - |
? | |
2.7.1.7 | ATP + D-glucose | 50% of the activity with D-mannose | Escherichia coli | ADP + D-glucose 6-phosphate | - |
? | |
2.7.1.7 | ATP + D-mannose | - |
Escherichia coli | ADP + D-mannose 6-phosphate | - |
? | |
2.7.1.7 | ATP + D-mannose | - |
Escherichia coli | ADP + mannose 6-phosphate | - |
? | |
2.7.1.7 | ATP + mannoheptulose | no activity | Escherichia coli | ADP + mannoheptulose 6-phosphate | - |
? | |
2.7.1.7 | CTP + D-mannose | 15% of the activity with ATP | Escherichia coli | CDP + D-mannose 6-phosphate | - |
? | |
2.7.1.7 | dATP + D-mannose | - |
Escherichia coli | dADP + D-mannose 6-phosphate | - |
? | |
2.7.1.7 | GTP + D-mannose | 60% of the activity with ATP | Escherichia coli | GDP + D-mannose 6-phosphate | - |
? | |
2.7.1.7 | ITP + D-mannose | as active as ATP | Escherichia coli | IDP + D-mannose 6-phosphate | - |
? | |
2.7.1.7 | TTP + D-mannose | 30% of the activity with ATP | Escherichia coli | TDP + D-mannose 6-phosphate | - |
? | |
2.7.1.7 | UTP + D-mannose | 60% of the activity with ATP | Escherichia coli | UDP + D-mannose 6-phosphate | - |
? |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.7.1.7 | 57 | - |
5 min, crude extract stable | Escherichia coli |
2.7.1.7 | 61 | - |
5 min, crude extract, 10% loss of activity | Escherichia coli |
2.7.1.7 | 64 | - |
5 min, crude extract, about 80% loss of activity | Escherichia coli |
2.7.1.7 | 66 | - |
5 min, crude extract, complete loss of activity | Escherichia coli |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.7.1.7 | additional information | - |
- |
Escherichia coli |
EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|---|
2.7.1.7 | 4 | - |
5 min, 50% loss of activity, 15 min, complete loss of activity | Escherichia coli |
2.7.1.7 | 5 | 10 | 25°C, 60 min, stable | Escherichia coli |
EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.7.1.7 | 0.1 | - |
MgADP- | - |
Escherichia coli | |
2.7.1.7 | 0.1 | - |
D-mannosamine | pH 7.5, 25°C | Escherichia coli | |
2.7.1.7 | 2 | - |
L-sorbose | pH 7.5, 25°C | Escherichia coli | |
2.7.1.7 | 5 | - |
lyxose | pH 7.5, 25°C | Escherichia coli |