BRENDA - Enzyme Database

Manganese-stimulated phosphatidylinositol 4-kinase from Dunaliella parva: purification and characterization

Steinert, P.; Wissing, J.B.; Wagner, K.G.; Plant Sci. 101, 105-114 (1994)
No PubMed abstract available

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
2.7.1.67
Triton X-100
activity depends on presence of a surfactant. Optimal concentration is 3.7 mM
Dunaliella parva
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
2.7.1.67
adenosine
-
Dunaliella parva
2.7.1.67
ADP
-
Dunaliella parva
2.7.1.67
AMP
-
Dunaliella parva
2.7.1.67
cAMP
-
Dunaliella parva
2.7.1.67
CDP
-
Dunaliella parva
2.7.1.67
CTP
-
Dunaliella parva
2.7.1.67
GTP
-
Dunaliella parva
2.7.1.67
phosphatidylethanolamine
-
Dunaliella parva
2.7.1.67
UTP
-
Dunaliella parva
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.7.1.67
0.027
-
phosphatidylinositol
pH 7.8, 28°C, at 0.75 mM Triton X-100
Dunaliella parva
2.7.1.67
0.055
-
phosphatidylinositol
pH 7.8, 28°C, at 1.5 mM Triton X-100
Dunaliella parva
2.7.1.67
0.39
-
ATP
pH 7.8, 28°C, at 2 mM MgCl2 and 10 mM MnCl2
Dunaliella parva
2.7.1.67
1.7
-
ATP
pH 7.8, 28°C, at 30 mM MgCl2
Dunaliella parva
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.7.1.67
soluble
-
Dunaliella parva
-
-
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
2.7.1.67
Ca2+
enzyme requires divalent cations, Mg2+ with an optimal concentration of 30 mM, Ca2+ with an optimal concentration of 10 mM or Mn2+ with an optimal concentration of 10 mM. Mn2+ is the best activator. Mn2+, Mg2+ or Ca2+ activate synergistically
Dunaliella parva
2.7.1.67
Mg2+
enzyme requires divalent cations, Mg2+ with an optimal concentration of 30 mM, Ca2+ with an optimal concentration of 10 mM or Mn2+ with an optimal concentration of 10 mM. Mn2+ is the best activator. Mn2+, Mg2+ or Ca2+ activate synergistically
Dunaliella parva
2.7.1.67
Mn2+
enzyme requires divalent cations, Mg2+ with an optimal concentration of 30 mM, Ca2+ with an optimal concentration of 10 mM or Mn2+ with an optimal concentration of 10 mM. Mn2+ is the best activator. Mn2+, Mg2+ or Ca2+ activate synergistically
Dunaliella parva
2.7.1.67
NaCl
enhances activity up to about 1.3fold at 1 M
Dunaliella parva
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.7.1.67
Dunaliella parva
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
2.7.1.67
partial
Dunaliella parva
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.7.1.67
0.077
-
-
Dunaliella parva
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.1.67
ATP + 1-phosphatidyl-1D-myo-inositol
-
641804
Dunaliella parva
ADP + 1-phosphatidyl-1D-myo-inositol 4-phosphate
-
-
-
?
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
2.7.1.67
28
-
at 30 mM MgCl2
Dunaliella parva
2.7.1.67
42
-
at 2 mM MgCl2 and 10 mM MnCl2
Dunaliella parva
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.7.1.67
7.8
-
-
Dunaliella parva
Ki Value [mM]
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
2.7.1.67
0.025
-
ADP
pH 7.8, 28°C
Dunaliella parva
2.7.1.67
0.775
-
adenosine
pH 7.8, 28°C
Dunaliella parva
2.7.1.67
1.2
-
AMP
pH 7.8, 28°C
Dunaliella parva
2.7.1.67
1.68
-
cAMP
pH 7.8, 28°C
Dunaliella parva
2.7.1.67
1.68
-
CTP
pH 7.8, 28°C
Dunaliella parva
2.7.1.67
1.75
-
GTP
pH 7.8, 28°C
Dunaliella parva
2.7.1.67
2.9
-
CDP
pH 7.8, 28°C
Dunaliella parva
2.7.1.67
3.6
-
UTP
pH 7.8, 28°C
Dunaliella parva
pI Value
EC Number
Organism
Commentary
pI Value Maximum
pI Value
2.7.1.67
Dunaliella parva
chromatofocusing
-
5.5
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
2.7.1.67
Triton X-100
activity depends on presence of a surfactant. Optimal concentration is 3.7 mM
Dunaliella parva
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
2.7.1.67
adenosine
-
Dunaliella parva
2.7.1.67
ADP
-
Dunaliella parva
2.7.1.67
AMP
-
Dunaliella parva
2.7.1.67
cAMP
-
Dunaliella parva
2.7.1.67
CDP
-
Dunaliella parva
2.7.1.67
CTP
-
Dunaliella parva
2.7.1.67
GTP
-
Dunaliella parva
2.7.1.67
phosphatidylethanolamine
-
Dunaliella parva
2.7.1.67
UTP
-
Dunaliella parva
Ki Value [mM] (protein specific)
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
2.7.1.67
0.025
-
ADP
pH 7.8, 28°C
Dunaliella parva
2.7.1.67
0.775
-
adenosine
pH 7.8, 28°C
Dunaliella parva
2.7.1.67
1.2
-
AMP
pH 7.8, 28°C
Dunaliella parva
2.7.1.67
1.68
-
cAMP
pH 7.8, 28°C
Dunaliella parva
2.7.1.67
1.68
-
CTP
pH 7.8, 28°C
Dunaliella parva
2.7.1.67
1.75
-
GTP
pH 7.8, 28°C
Dunaliella parva
2.7.1.67
2.9
-
CDP
pH 7.8, 28°C
Dunaliella parva
2.7.1.67
3.6
-
UTP
pH 7.8, 28°C
Dunaliella parva
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.7.1.67
0.027
-
phosphatidylinositol
pH 7.8, 28°C, at 0.75 mM Triton X-100
Dunaliella parva
2.7.1.67
0.055
-
phosphatidylinositol
pH 7.8, 28°C, at 1.5 mM Triton X-100
Dunaliella parva
2.7.1.67
0.39
-
ATP
pH 7.8, 28°C, at 2 mM MgCl2 and 10 mM MnCl2
Dunaliella parva
2.7.1.67
1.7
-
ATP
pH 7.8, 28°C, at 30 mM MgCl2
Dunaliella parva
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.7.1.67
soluble
-
Dunaliella parva
-
-
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
2.7.1.67
Ca2+
enzyme requires divalent cations, Mg2+ with an optimal concentration of 30 mM, Ca2+ with an optimal concentration of 10 mM or Mn2+ with an optimal concentration of 10 mM. Mn2+ is the best activator. Mn2+, Mg2+ or Ca2+ activate synergistically
Dunaliella parva
2.7.1.67
Mg2+
enzyme requires divalent cations, Mg2+ with an optimal concentration of 30 mM, Ca2+ with an optimal concentration of 10 mM or Mn2+ with an optimal concentration of 10 mM. Mn2+ is the best activator. Mn2+, Mg2+ or Ca2+ activate synergistically
Dunaliella parva
2.7.1.67
Mn2+
enzyme requires divalent cations, Mg2+ with an optimal concentration of 30 mM, Ca2+ with an optimal concentration of 10 mM or Mn2+ with an optimal concentration of 10 mM. Mn2+ is the best activator. Mn2+, Mg2+ or Ca2+ activate synergistically
Dunaliella parva
2.7.1.67
NaCl
enhances activity up to about 1.3fold at 1 M
Dunaliella parva
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.7.1.67
partial
Dunaliella parva
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.7.1.67
0.077
-
-
Dunaliella parva
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.1.67
ATP + 1-phosphatidyl-1D-myo-inositol
-
641804
Dunaliella parva
ADP + 1-phosphatidyl-1D-myo-inositol 4-phosphate
-
-
-
?
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
2.7.1.67
28
-
at 30 mM MgCl2
Dunaliella parva
2.7.1.67
42
-
at 2 mM MgCl2 and 10 mM MnCl2
Dunaliella parva
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.7.1.67
7.8
-
-
Dunaliella parva
pI Value (protein specific)
EC Number
Organism
Commentary
pI Value Maximum
pI Value
2.7.1.67
Dunaliella parva
chromatofocusing
-
5.5