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Literature summary extracted from

  • Kanoh, H.; Banno, Y.; Hirata, M.; Nozawa, Y.
    Partial purification and characterization of phosphatidylinositol kinases from human platelets (1990), Biochim. Biophys. Acta, 1046, 120-126.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.1.67 Triton X-100 inhibits activity of mPIK-I but rather weakly enhances mPIK-III activity Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.67 ADP inhibition of mPIK-I and mPIK-III Homo sapiens
2.7.1.67 arachidonic acid 0.5 mg/ml, 91.3% inhibition of mPIK-III Homo sapiens
2.7.1.67 phosphatidylserine 0.5 mg/ml, 64.3% inhibition of mPIK-III Homo sapiens
2.7.1.67 sodium cholate inhibits activity of both mPIK-I and mPIK-III Homo sapiens
2.7.1.67 Triton X-100 inhibits activity of mPIK-I but rather weakly enhances mPIK-III activity Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.67 0.075
-
 ATP pH 7.4, 30°C, enzyme form mPIK-III Homo sapiens
2.7.1.67 0.152
-
 ATP pH 7.4, 30°C, enzyme form cPIK-I and cPIK-II Homo sapiens
2.7.1.67 0.217
-
 ATP pH 7.4, 30°C, enzyme form mPIK-I Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.1.67 cytosol enzyme form cPIK-I and cPIK-II Homo sapiens 5829
-
2.7.1.67 membrane enzyme form mPIK-I and mPIK-II Homo sapiens 16020
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.67 Mg2+ required, optimal concentrations for mPIK-I and mPIK-III at 5-10 mM, optimal concentration for cPIK-I and cPIK-II is 20-40 mM Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.67 76000
-
 mPIK-I, gel filtration Homo sapiens
2.7.1.67 80000
-
 mPIK-III, gel filtration Homo sapiens
2.7.1.67 550000
-
 cPIK-I and cPIK-II, gel filtration Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.67 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.67 mPIK-I and mPIK-III from membrane fraction and cPIK-I and cPIK-II from cytoslic fraction Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.1.67 platelet
-
 Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.67 ATP + 1-phosphatidyl-1D-myo-inositol
-
 Homo sapiens ADP + 1-phosphatidyl-1D-myo-inositol 4-phosphate
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.67 7.5
-
 cPIK-I and cPIK-II Homo sapiens
2.7.1.67 8.5 9 mPIK-I and mPIK-III Homo sapiens

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.7.1.67 5.5 9 pH 5.5: 60% of maximal activity for mPIK-I and 45% of maximal activity of mPIK-III, pH 8.5-9.0: pH-optimum for enzyme form mPIK-I and mPIK-III Homo sapiens
2.7.1.67 6 9 activity of cPIK-I and cPIK-III is almost undetectable at pH 6.0, pH 9.0: about 80% of maximal activity Homo sapiens