BRENDA - Enzyme Database

Purification and characterization of phosphatidylinositol kinase from porcine liver microsomes

Hou, W.M.; Zhang, Z.L.; Tai, H.H.; Biochim. Biophys. Acta 959, 67-75 (1988)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
2.7.1.67
adenosine
IC50: 0.07 mM. 1 mM, 93% inhibition
Sus scrofa
2.7.1.67
ADP
IC50: 0.12 mM. 1 mM, 92% inhibition
Sus scrofa
2.7.1.67
AMP
1 mM, 48% inhibition
Sus scrofa
2.7.1.67
Ca2+
inhibits Mg2+-stimulated activity, IC50: 0.4 mM
Sus scrofa
2.7.1.67
deoxycholate
10 mM, 35% inhibition
Sus scrofa
2.7.1.67
GDP
1 mM, 27% inhibition
Sus scrofa
2.7.1.67
GMP
1 mM, 20% inhibition
Sus scrofa
2.7.1.67
GTP
1 mM, 24% inhibition
Sus scrofa
2.7.1.67
guanosine
1 mM, 50% inhibition
Sus scrofa
2.7.1.67
Lubrol Px
1%, 72% inhibition
Sus scrofa
2.7.1.67
NEM
0.1 mM, 42% inhibition
Sus scrofa
2.7.1.67
PCMB
0.1 mM, 98% inhibition
Sus scrofa
2.7.1.67
phosphatidylethanolamine
0.1 mM, 24% inhibition
Sus scrofa
2.7.1.67
phosphatidylglycerol
0.1 mM, 63% inhibition
Sus scrofa
2.7.1.67
phosphatidylserine
0.1 mM, 33% inhibition
Sus scrofa
2.7.1.67
quercetin
IC50: 0.1 mM
Sus scrofa
2.7.1.67
Sodium mersalyl
0.1 mM, 78% inhibition
Sus scrofa
2.7.1.67
Triton X-100
0.2%, 11% inhibition
Sus scrofa
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.7.1.67
0.06
-
ATP
pH 7.5, 30░C
Sus scrofa
2.7.1.67
0.12
-
phosphatidylinositol
pH 7.5, 30░C
Sus scrofa
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.7.1.67
microsome
-
Sus scrofa
-
-
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
2.7.1.67
Co2+
divalent metal ions stimulate in decreasing order of efficiency: Mg2+, Fe2+, Mn2+, Fe3+, Co2+
Sus scrofa
2.7.1.67
Fe2+
divalent metal ions stimulate in decreasing order of efficiency: Mg2+, Fe2+, Mn2+, Fe3+, Co2+
Sus scrofa
2.7.1.67
Fe3+
divalent metal ions stimulate in decreasing order of efficiency: Mg2+, Fe2+, Mn2+, Fe3+, Co2+
Sus scrofa
2.7.1.67
Mg2+
divalent metal ions stimulate in decreasing order of efficiency: Mg2+, Fe2+, Mn2+, Fe3+, Co2+
Sus scrofa
2.7.1.67
Mn2+
divalent metal ions stimulate in decreasing order of efficiency: Mg2+, Fe2+, Mn2+, Fe3+, Co2+
Sus scrofa
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.7.1.67
55000
-
x * 55000, SDS-PAGE
Sus scrofa
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.7.1.67
Sus scrofa
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
2.7.1.67
-
Sus scrofa
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
2.7.1.67
liver
-
Sus scrofa
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
2.7.1.67
8.824
-
-
Sus scrofa
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.1.67
ATP + 1-phosphatidyl-1D-myo-inositol
-
641791
Sus scrofa
ADP + 1-phosphatidyl-1D-myo-inositol 4-phosphate
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
2.7.1.67
?
x * 55000, SDS-PAGE
Sus scrofa
IC50 Value
EC Number
IC50 Value
IC50 Value Maximum
Commentary
Organism
Inhibitor
Structure
2.7.1.67
0.07
-
IC50: 0.07 mM. 1 mM, 93% inhibition
Sus scrofa
adenosine
2.7.1.67
0.1
-
IC50: 0.1 mM
Sus scrofa
quercetin
2.7.1.67
0.12
-
IC50: 0.12 mM. 1 mM, 92% inhibition
Sus scrofa
ADP
2.7.1.67
0.4
-
inhibits Mg2+-stimulated activity, IC50: 0.4 mM
Sus scrofa
Ca2+
IC50 Value (protein specific)
EC Number
IC50 Value
IC50 Value Maximum
Commentary
Organism
Inhibitor
Structure
2.7.1.67
0.07
-
IC50: 0.07 mM. 1 mM, 93% inhibition
Sus scrofa
adenosine
2.7.1.67
0.1
-
IC50: 0.1 mM
Sus scrofa
quercetin
2.7.1.67
0.12
-
IC50: 0.12 mM. 1 mM, 92% inhibition
Sus scrofa
ADP
2.7.1.67
0.4
-
inhibits Mg2+-stimulated activity, IC50: 0.4 mM
Sus scrofa
Ca2+
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
2.7.1.67
adenosine
IC50: 0.07 mM. 1 mM, 93% inhibition
Sus scrofa
2.7.1.67
ADP
IC50: 0.12 mM. 1 mM, 92% inhibition
Sus scrofa
2.7.1.67
AMP
1 mM, 48% inhibition
Sus scrofa
2.7.1.67
Ca2+
inhibits Mg2+-stimulated activity, IC50: 0.4 mM
Sus scrofa
2.7.1.67
deoxycholate
10 mM, 35% inhibition
Sus scrofa
2.7.1.67
GDP
1 mM, 27% inhibition
Sus scrofa
2.7.1.67
GMP
1 mM, 20% inhibition
Sus scrofa
2.7.1.67
GTP
1 mM, 24% inhibition
Sus scrofa
2.7.1.67
guanosine
1 mM, 50% inhibition
Sus scrofa
2.7.1.67
Lubrol Px
1%, 72% inhibition
Sus scrofa
2.7.1.67
NEM
0.1 mM, 42% inhibition
Sus scrofa
2.7.1.67
PCMB
0.1 mM, 98% inhibition
Sus scrofa
2.7.1.67
phosphatidylethanolamine
0.1 mM, 24% inhibition
Sus scrofa
2.7.1.67
phosphatidylglycerol
0.1 mM, 63% inhibition
Sus scrofa
2.7.1.67
phosphatidylserine
0.1 mM, 33% inhibition
Sus scrofa
2.7.1.67
quercetin
IC50: 0.1 mM
Sus scrofa
2.7.1.67
Sodium mersalyl
0.1 mM, 78% inhibition
Sus scrofa
2.7.1.67
Triton X-100
0.2%, 11% inhibition
Sus scrofa
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.7.1.67
0.06
-
ATP
pH 7.5, 30░C
Sus scrofa
2.7.1.67
0.12
-
phosphatidylinositol
pH 7.5, 30░C
Sus scrofa
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.7.1.67
microsome
-
Sus scrofa
-
-
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
2.7.1.67
Co2+
divalent metal ions stimulate in decreasing order of efficiency: Mg2+, Fe2+, Mn2+, Fe3+, Co2+
Sus scrofa
2.7.1.67
Fe2+
divalent metal ions stimulate in decreasing order of efficiency: Mg2+, Fe2+, Mn2+, Fe3+, Co2+
Sus scrofa
2.7.1.67
Fe3+
divalent metal ions stimulate in decreasing order of efficiency: Mg2+, Fe2+, Mn2+, Fe3+, Co2+
Sus scrofa
2.7.1.67
Mg2+
divalent metal ions stimulate in decreasing order of efficiency: Mg2+, Fe2+, Mn2+, Fe3+, Co2+
Sus scrofa
2.7.1.67
Mn2+
divalent metal ions stimulate in decreasing order of efficiency: Mg2+, Fe2+, Mn2+, Fe3+, Co2+
Sus scrofa
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.7.1.67
55000
-
x * 55000, SDS-PAGE
Sus scrofa
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.7.1.67
-
Sus scrofa
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
2.7.1.67
liver
-
Sus scrofa
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
2.7.1.67
8.824
-
-
Sus scrofa
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.1.67
ATP + 1-phosphatidyl-1D-myo-inositol
-
641791
Sus scrofa
ADP + 1-phosphatidyl-1D-myo-inositol 4-phosphate
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
2.7.1.67
?
x * 55000, SDS-PAGE
Sus scrofa