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Literature summary extracted from

  • Endemann, G.; Dunn, S.N.; Cantley, L.C.
    Bovine brain contains two types of phosphatidylinositol kinase (1987), Biochemistry, 26, 6845-6852.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.1.67 Triton X-100 type 2 and type 3 enzyme are maximally active in 0.1-0.5% Triton X-100 Bos taurus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.67 adenosine
-
 Bos taurus
2.7.1.67 ADP
-
 Bos taurus
2.7.1.67 AMP
-
 Bos taurus
2.7.1.67 phosphatidylcholine inhibits type 3 kinase more strongly than type 2 kinase Bos taurus
2.7.1.67 phosphatidylinositol 4-phosphate
-
 Bos taurus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.67 0.017
-
 phosphatidylinositol room temperature, enzyme type 2 Bos taurus
2.7.1.67 0.054
-
 ATP room temperature, enzyme type 2 Bos taurus
2.7.1.67 0.127
-
 phosphatidylinositol room temperature, enzyme type 3 Bos taurus
2.7.1.67 0.742
-
 ATP room temperature, enzyme type 3 Bos taurus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.67 Mg2+ Km for type 2 kinase is 0.6 mM, Km for type 3 kinase is 2.6 mM Bos taurus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.67 55000
-
 enzyme type 2, sucrose density gradient centrifugation Bos taurus
2.7.1.67 230000
-
 enzyme type 3, sucrose density gradient centrifugation Bos taurus

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.67 Bos taurus
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.1.67 brain
-
 Bos taurus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.67 ATP + 1-phosphatidyl-1D-myo-inositol
-
 Bos taurus ADP + 1-phosphatidyl-1D-myo-inositol 4-phosphate
-
 ?

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.7.1.67 0.018
-
 adenosine room temperature, enzyme type 2 Bos taurus
2.7.1.67 0.027
-
 AMP room temperature, enzyme type 2 Bos taurus
2.7.1.67 0.235
-
 phosphatidylinositol 4-phosphate room temperature Bos taurus
2.7.1.67 0.24
-
 AMP room temperature, enzyme type 2 Bos taurus
2.7.1.67 0.57
-
 ADP room temperature, enzyme type 3 Bos taurus
2.7.1.67 0.774
-
 phosphatidylinositol 4-phosphate room temperature Bos taurus
2.7.1.67 1.52
-
 adenosine room temperature, enzyme type 3 Bos taurus
2.7.1.67 2.82
-
 AMP room temperature, enzyme type 3 Bos taurus