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Literature summary extracted from

  • Hsieh, P.C.; Shenoy, B.C.; Samols, D.; Phillips, N.F.
    Cloning, expression, and characterization of polyphosphate glucokinase from Mycobacterium tuberculosis (1996), J. Biol. Chem., 271, 4909-4915.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.63 expression in Escherichia coli Mycobacterium tuberculosis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.63 27400
-
 x * 27400, calculation from nucleotide sequence Mycobacterium tuberculosis
2.7.1.63 33000
-
 x * 33000, SDS-PAGE Mycobacterium tuberculosis

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.63 Mycobacterium tuberculosis P9WIN1
-
-
2.7.1.63 Mycobacterium tuberculosis H37Rv P9WIN1
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.63 recombinant enzyme Mycobacterium tuberculosis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.63 203
-
 polyphosphate-dependent activity Mycobacterium tuberculosis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.63 (phosphate)n + D-glucose
-
 Mycobacterium tuberculosis (phosphate)n-1 + D-glucose 6-phosphate
-
 ?
2.7.1.63 (phosphate)n + D-glucose
-
 Mycobacterium tuberculosis H37Rv (phosphate)n-1 + D-glucose 6-phosphate
-
 ?
2.7.1.63 ATP + D-glucose
-
 Mycobacterium tuberculosis ADP + D-glucose 6-phosphate
-
 ?
2.7.1.63 ATP + D-glucose
-
 Mycobacterium tuberculosis H37Rv ADP + D-glucose 6-phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.1.63 ? x * 33000, SDS-PAGE Mycobacterium tuberculosis
2.7.1.63 ? x * 27400, calculation from nucleotide sequence Mycobacterium tuberculosis