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Literature summary extracted from

  • Barkulis, S.S.
    N-Acetyl-D-glucosamine kinase. I. Steptococcus pyrogenes (1966), Methods Enzymol., 9, 415-420.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.1.59 cysteine reverses inhibition with p-hydroxymercuribenzoate Streptococcus pyogenes

General Stability

EC Number General Stability Organism
2.7.1.59 ammonium sulfate partially stabilizes against temperature inactivation Streptococcus pyogenes
2.7.1.59 dialysis at 4°C for 17 h against 0.005 M Tris buffer, pH 7.6, 92% loss of activity, 33% loss of activity in presence of 0.1 M N-acetyl-D-glucosamine or glucose, stable in presence of 0.2 M or 2.5 Streptococcus pyogenes
2.7.1.59 substrate at 0.2 M stabilizes, unstable in absence of substrate at 4°C Streptococcus pyogenes

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.59 D-glucose competitive with N-acetyl-D-glucosamine Streptococcus pyogenes
2.7.1.59 N-acetyl-D-glucosamine competitive with glucose Streptococcus pyogenes
2.7.1.59 p-hydroxymercuribenzoate no protection by substrate, protection but not reversal of inhibition by thioethanol, complete inhibition above 0.00125 mM, cysteine reverses inhibition Streptococcus pyogenes

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.59 0.00077
-
 N-acetyl-D-glucosamine 30°C, pH 7.4 Streptococcus pyogenes
2.7.1.59 0.0011
-
 D-glucose 30°C, pH 7.4 Streptococcus pyogenes

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.59 ATP + N-acetyl-D-glucosamine Streptococcus pyogenes
-
 ADP + N-acetyl-D-glucosamine 6-phosphate
-
 ?
2.7.1.59 ATP + N-acetyl-D-glucosamine Streptococcus pyogenes 523
-
 ADP + N-acetyl-D-glucosamine 6-phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.59 Streptococcus pyogenes
-
 serotype 14
-
2.7.1.59 Streptococcus pyogenes 523
-
 serotype 14
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.59
-
 Streptococcus pyogenes

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.59 250
-
 purified enzyme, 30°C, pH 7.4 Streptococcus pyogenes

Storage Stability

EC Number Storage Stability Organism
2.7.1.59 -25°C, stable for several months Streptococcus pyogenes

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.59 ATP + D-glucose at equal rate than N-acetyl-D-glucosamine Streptococcus pyogenes ADP + D-glucose 6-phosphate
-
 ?
2.7.1.59 ATP + D-glucose at equal rate than N-acetyl-D-glucosamine Streptococcus pyogenes 523 ADP + D-glucose 6-phosphate
-
 ?
2.7.1.59 ATP + N-acetyl-D-glucosamine
-
 Streptococcus pyogenes ADP + N-acetyl-D-glucosamine 6-phosphate
-
 ?
2.7.1.59 ATP + N-acetyl-D-glucosamine
-
 Streptococcus pyogenes 523 ADP + N-acetyl-D-glucosamine 6-phosphate
-
 ?
2.7.1.59 additional information D-fructose, D-mannose, D-maltose, D-galactose, N-acetyl-D-galactosamine, N-acetyl-D-mannosamine do not serve as substrates Streptococcus pyogenes ?
-
 ?
2.7.1.59 additional information D-fructose, D-mannose, D-maltose, D-galactose, N-acetyl-D-galactosamine, N-acetyl-D-mannosamine do not serve as substrates Streptococcus pyogenes 523 ?
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.7.1.59 60
-
 25% loss of activity within 1 min in presence of 13% ammonium sulfate, stable for 1 h in presence of ammonium sulfate and substrate Streptococcus pyogenes
2.7.1.59 70
-
 complete inactivation with 1 min Streptococcus pyogenes

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.59 7 8 strongly decreasing activity outside this range Streptococcus pyogenes

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.7.1.59 0.0009
-
 N-acetyl-D-glucosamine for reaction with glucose, 30°C, pH 7.4 Streptococcus pyogenes
2.7.1.59 0.0018
-
 D-glucose for reaction with N-acetyl-D-glucosamine, 30°C, pH 7.4 Streptococcus pyogenes