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Literature summary extracted from

  • Park, S.H.; Pastuszak, I.; Drake, R.; Elbein, A.D.
    Purification to apparent homogeneity and properties of pig kidney L-fucose kinase (1998), J. Biol. Chem., 273, 5685-5691.
    View publication on PubMed

Application

EC Number Application Comment Organism
2.7.1.52 synthesis the enzyme is a valuable biochemical tool to prepare activated L-fucose derivatives for fucosylation reactions, enzyme is valuable for the formation of radiolabeled fucose 1-phosphate Sus scrofa

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.52 D-arabinose 0.5 mM, 20% inhibition. 10 mM, 84% inhibition Sus scrofa
2.7.1.52 GDP-L-fucose
-
Sus scrofa
2.7.1.52 L-fucose 0.5 mM Sus scrofa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.52 0.027
-
L-fucose pH 8.0, 37°C Sus scrofa
2.7.1.52 0.6
-
ATP pH 8.0, 37°C Sus scrofa

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.1.52 cytosol
-
Sus scrofa 5829
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.52 Co2+ divalent cation required, Co2+ stimulates with maximal activity at 3-5 mM, maximal activity is about 35-30% compared to activation by Mg2+ Sus scrofa
2.7.1.52 Fe2+ divalent cation required, Fe2+ stimulates nearly to the same degree as Mg2+, optimum activity at 10 mM Sus scrofa
2.7.1.52 Mg2+ absolute requirement for a divalent cation, best stimulation at 3 mM Sus scrofa
2.7.1.52 Mn2+ divalent cation required, Mn2+ stimulates with maximal activity at 3-5 mM, maximal activity is about 35-30% compared to activation by Mg2+ Sus scrofa

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.52 110000
-
4 * 110000, SDS-PAGE Sus scrofa
2.7.1.52 440000
-
gel filtration Sus scrofa

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.52 ATP + 6-deoxy-L-galactose Sus scrofa enzyme is part of a salvage pathway for reutilization of L-fucose ADP + 6-deoxy-L-galactose 1-phosphate
-
?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.52 Sus scrofa
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.52
-
Sus scrofa

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.1.52 aorta
-
Sus scrofa
-
2.7.1.52 brain
-
Sus scrofa
-
2.7.1.52 heart
-
Sus scrofa
-
2.7.1.52 kidney
-
Sus scrofa
-
2.7.1.52 liver
-
Sus scrofa
-
2.7.1.52 lung
-
Sus scrofa
-
2.7.1.52 pancreas
-
Sus scrofa
-
2.7.1.52 spleen
-
Sus scrofa
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.52 1.273
-
-
Sus scrofa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.52 ATP + 6-deoxy-L-galactose
-
Sus scrofa ADP + 6-deoxy-L-galactose 1-phosphate beta-L-fucose 1-phosphate ?
2.7.1.52 ATP + 6-deoxy-L-galactose enzyme is part of a salvage pathway for reutilization of L-fucose Sus scrofa ADP + 6-deoxy-L-galactose 1-phosphate
-
?
2.7.1.52 ATP + D-arabinose 9.3% of the activity with L-fucose Sus scrofa ?
-
?
2.7.1.52 ATP + D-glucose 2.5% of the activity with L-fucose Sus scrofa ?
-
?
2.7.1.52 ITP + 6-deoxy-L-galactose 2.5% of the activity with L-fucose Sus scrofa IDP + 6-deoxy-L-galactose 1-phosphate
-
?

Subunits

EC Number Subunits Comment Organism
2.7.1.52 tetramer 4 * 110000, SDS-PAGE Sus scrofa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.52 8
-
in Tris buffer about 80% of the activity in HEPES buffer Sus scrofa

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.7.1.52 0.01
-
GDP-L-fucose
-
Sus scrofa