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Literature summary extracted from

  • Lewin, L.M.; Brown, G.M.
    The biosynthesis of thiamine. III. Mechanism of enzymatic formation of the pyrophosphate ester of 2-methyl-4-amino-5-hydroxymethylpyrimidine (1961), J. Biol. Chem., 236, 2768-2771.
No PubMed abstract available

General Stability

EC Number General Stability Organism
2.7.1.49 protected from inactivation by cysteine Saccharomyces cerevisiae

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.49 additional information stable to treatment with p-hydroxymercuribenzoate Saccharomyces cerevisiae
2.7.4.7 p-hydroxymercuribenzoate 1 mM, complete inhibition Saccharomyces cerevisiae

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.49 additional information no metal requirement Saccharomyces cerevisiae
2.7.4.7 Mg2+ required for activity Saccharomyces cerevisiae
2.7.4.7 Mn2+ may substitute for Mg2+ Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.49 ATP + 2-methyl-4-amino-5-hydroxymethylpyrimidine Saccharomyces cerevisiae involved in thiamine biosynthetic pathway, first steps ADP + 2-methyl-4-amino-5-phosphomethylpyrimidine
-
 ?
2.7.4.7 ATP + 2-methyl-4-amino-5-phosphomethylpyrimidine Saccharomyces cerevisiae specific for ATP ADP + 2-methyl-4-amino-5-diphosphomethylpyrimidine product demonstrated in crude extract ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.49 Saccharomyces cerevisiae
-
 bakers' yeast
-
2.7.4.7 Saccharomyces cerevisiae
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.49 partially Saccharomyces cerevisiae
2.7.4.7 partial Saccharomyces cerevisiae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.49 ATP + 2-methyl-4-amino-5-hydroxymethylpyrimidine
-
 Saccharomyces cerevisiae ADP + 2-methyl-4-amino-5-phosphomethylpyrimidine
-
 ?
2.7.1.49 ATP + 2-methyl-4-amino-5-hydroxymethylpyrimidine involved in thiamine biosynthetic pathway, first steps Saccharomyces cerevisiae ADP + 2-methyl-4-amino-5-phosphomethylpyrimidine
-
 ?
2.7.1.49 ATP + 4-amino-5-hydroxy-2-methylpyrimidine
-
 Saccharomyces cerevisiae ADP + 4-amino-2-methyl-5-phosphopyrimidine
-
 ?
2.7.1.49 CTP + 5-hydroxy-2-methylpyrimidine
-
 Saccharomyces cerevisiae CDP + 2-methyl-5-phosphopyrimidine
-
 ?
2.7.1.49 GTP + 5-hydroxy-2-methylpyrimidine
-
 Saccharomyces cerevisiae GDP + 2-methyl-5-phosphopyrimidine
-
 ?
2.7.1.49 UTP + 5-hydroxy-2-methylpyrimidine
-
 Saccharomyces cerevisiae UDP + 5-hydroxy-2-methylpyrimidine monophosphate
-
 ?
2.7.4.7 ATP + 2-methyl-4-amino-5-phosphomethylpyrimidine specific for ATP Saccharomyces cerevisiae ADP + 2-methyl-4-amino-5-diphosphomethylpyrimidine product demonstrated in crude extract ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.4.7 38
-
 assay at Saccharomyces cerevisiae

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.7.1.49 55
-
 stable to heating for 10 min Saccharomyces cerevisiae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.4.7 7
-
 assay at Saccharomyces cerevisiae