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Literature summary extracted from

  • Anderson, E.P.
    Uridine-cytidine kinase from a murine neoplasm (1978), Methods Enzymol., 51, 314-321.
    View publication on PubMed

General Stability

EC Number General Stability Organism
2.7.1.48 some loss of activity after repeated freezing and thawing Mus musculus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.48 5'-Amino-5-deoxyuridine
-
 Mus musculus
2.7.1.48 5'-Azido-5-deoxyuridine
-
 Mus musculus
2.7.1.48 5'-Deoxyuridine
-
 Mus musculus
2.7.1.48 5'-Iodo-5'-deoxyuridine
-
 Mus musculus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.48 0.045
-
 cytidine pH 7.5, 25°C Mus musculus
2.7.1.48 0.15
-
 uridine pH 7.5, 25°C Mus musculus
2.7.1.48 2.1
-
 ATP pH 7.5, 25°C, cosubstrate cytidine Mus musculus
2.7.1.48 3.6
-
 ATP pH 7.5, 25°C, cosubstrate uridine Mus musculus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.48 Co2+ 13 mM, 66% of activity with Mg2+ Mus musculus
2.7.1.48 Fe2+ can partially replace Mg2+ Mus musculus
2.7.1.48 Fe2+ 13 mM, 65% of activity with Mg2+ Mus musculus
2.7.1.48 Mg2+ required for full activity Mus musculus
2.7.1.48 Mn2+ can partially replace Mg2+ Mus musculus
2.7.1.48 Mn2+ 13 mM, 80% of activity with Mg2+ Mus musculus
2.7.1.48 additional information not activated by Ca2+ and Cu2+ Mus musculus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.48 160000
-
 gel filtration Mus musculus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.48 ATP + uridine Mus musculus enzyme is part of the anabolic pathway by which the preformed pyrimidine nucleosides are salvaged for nucleic acid biosynthesis ADP + UMP
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.48 Mus musculus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.48 DEAE-cellulose, ammonium sulfate, hydroxyapatite Mus musculus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.1.48 mastocytoma cell P815 cell line Mus musculus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.48 3.03
-
-
 Mus musculus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.48 ATP + 5-azacytidine
-
 Mus musculus ADP + 5-azacytidine 5'-monophosphate
-
 ?
2.7.1.48 ATP + 5-fluorouridine
-
 Mus musculus ADP + 5-fluorouridine 5'-monophosphate
-
 ?
2.7.1.48 ATP + 6-azacytidine
-
 Mus musculus ADP + 6-azacytidine 5'-monophosphate
-
 ?
2.7.1.48 ATP + 6-azauridine
-
 Mus musculus ADP + 6-azauridine 5'-monophosphate
-
 ?
2.7.1.48 ATP + cytidine 50% of activity with uridine Mus musculus ADP + CMP
-
 ?
2.7.1.48 ATP + uridine no activity with thymine riboside, deoxyribosides, cytosine arabinoside, adenosine, purine deoxyribosides, UMP, CMP and deoxyribosides Mus musculus ADP + UMP
-
 ?
2.7.1.48 ATP + uridine enzyme is part of the anabolic pathway by which the preformed pyrimidine nucleosides are salvaged for nucleic acid biosynthesis Mus musculus ADP + UMP
-
 ?
2.7.1.48 dATP + uridine 88% of activity with ATP Mus musculus dADP + UMP
-
 ?
2.7.1.48 dCTP + uridine 50% of activity with ATP Mus musculus dCDP + UMP
-
 ?
2.7.1.48 dGTP + uridine 21% of activity with ATP Mus musculus dGDP + UMP
-
 ?
2.7.1.48 dUTP + uridine 67% of activity with ATP Mus musculus dUDP + UMP
-
 ?
2.7.1.48 GTP + uridine 23% of the activity with ATP Mus musculus UMP + GDP
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.48 6.5 7
-
 Mus musculus