Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Orengo, A.; Kobayashi, S.H.
    Uridine-cytidine kinase from Novikoff ascites rat tumor and Bacillus stearothermophilus (1978), Methods Enzymol., 51, 299-307.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.48 CTP enzyme shows a temperature dependence of CTP inhibition Geobacillus stearothermophilus
2.7.1.48 CTP 0.56 mM, 50% inhibition Rattus norvegicus
2.7.1.48 UTP
-
 Geobacillus stearothermophilus
2.7.1.48 UTP
-
 Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.48 0.21
-
 uridine pH 7.5, 10°C Geobacillus stearothermophilus
2.7.1.48 0.64
-
 uridine pH 7.5, 37°C Geobacillus stearothermophilus
2.7.1.48 1.6
-
 uridine pH 7.5, 60°C Geobacillus stearothermophilus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.48 Mg2+ required for full activity Rattus norvegicus
2.7.1.48 Mg2+ required for full activity Geobacillus stearothermophilus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.48 ATP + uridine Rattus norvegicus first enzyme of pyrimidine salvage pathway ADP + UMP
-
 ?
2.7.1.48 ATP + uridine Geobacillus stearothermophilus first enzyme of pyrimidine salvage pathway ADP + UMP
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.48 Geobacillus stearothermophilus
-
-
-
2.7.1.48 Rattus norvegicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.48
-
 Geobacillus stearothermophilus
2.7.1.48 streptomycin, ammonium sulfate, calcium phosphate, Sephadex G-200 Rattus norvegicus

Reaction

EC Number Reaction Comment Organism Reaction ID
2.7.1.48 ATP + uridine = ADP + UMP ping-pong mechanism Rattus norvegicus
a
2.7.1.48 ATP + uridine = ADP + UMP ping-pong mechanism Geobacillus stearothermophilus
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.1.48 Novikoff ascites tumor cell
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.48 0.88
-
-
 Rattus norvegicus
2.7.1.48 4.33
-
-
 Geobacillus stearothermophilus

Storage Stability

EC Number Storage Stability Organism
2.7.1.48 -20°C, 50% glycerol, at least 6 months, no loss of activity Rattus norvegicus
2.7.1.48 4°C, at least 1 month, no loss of activity Rattus norvegicus
2.7.1.48 4°C, sterile conditions, at least 2 years, no loss of activity enzyme Geobacillus stearothermophilus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.48 ATP + uridine very low activity with CTP and UTP Rattus norvegicus ADP + UMP
-
 ?
2.7.1.48 ATP + uridine very low activity with CTP and UTP Geobacillus stearothermophilus ADP + UMP
-
 ?
2.7.1.48 ATP + uridine first enzyme of pyrimidine salvage pathway Rattus norvegicus ADP + UMP
-
 ?
2.7.1.48 ATP + uridine first enzyme of pyrimidine salvage pathway Geobacillus stearothermophilus ADP + UMP
-
 ?
2.7.1.48 dATP + uridine
-
 Rattus norvegicus dADP + UMP
-
 ?
2.7.1.48 dATP + uridine
-
 Geobacillus stearothermophilus dADP + UMP
-
 ?
2.7.1.48 dCTP + uridine weak activity Rattus norvegicus dCDP + UMP
-
 ?
2.7.1.48 dCTP + uridine weak activity Geobacillus stearothermophilus dCDP + UMP
-
 ?
2.7.1.48 dGTP + uridine
-
 Rattus norvegicus dGDP + UMP
-
 ?
2.7.1.48 dGTP + uridine
-
 Geobacillus stearothermophilus dGDP + UMP
-
 ?
2.7.1.48 dTTP + uridine weak activity Rattus norvegicus dTDP + UMP
-
 ?
2.7.1.48 dTTP + uridine weak activity Geobacillus stearothermophilus dTDP + UMP
-
 ?
2.7.1.48 dUTP + uridine
-
 Rattus norvegicus dUDP + UMP
-
 ?
2.7.1.48 dUTP + uridine
-
 Geobacillus stearothermophilus dUDP + UMP
-
 ?
2.7.1.48 GTP + uridine weak activity Rattus norvegicus UMP + GDP
-
 ?
2.7.1.48 GTP + uridine weak activity Geobacillus stearothermophilus UMP + GDP
-
 ?
2.7.1.48 ITP + uridine weak activity Rattus norvegicus IDP + UMP
-
 ?
2.7.1.48 ITP + uridine weak activity Geobacillus stearothermophilus IDP + UMP
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.1.48 37
-
 assay at Rattus norvegicus
2.7.1.48 37
-
 assay at Geobacillus stearothermophilus
2.7.1.48 60
-
 higher activity than at 10°C and 37°C Geobacillus stearothermophilus