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Literature summary extracted from

  • Fromm, H.J.
    D-Ribulokinase from Aerobacter aerogenes (1959), J. Biol. Chem., 234, 3097-3101.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.1.47 2-mercaptoethanol activation Klebsiella aerogenes

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.47 Mg2+ inhibition at a Mg/ATP ratio above 2, 11% inhibition at a ratio of 3 Klebsiella aerogenes

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.47 0.83
-
 ATP pH 7.7, 27°C Klebsiella aerogenes
2.7.1.47 0.92
-
 D-ribulose pH 7.7, 37°C Klebsiella aerogenes

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.47 Ca2+ activation, can replace Mg2+ with 23% efficiency Klebsiella aerogenes
2.7.1.47 Mg2+ optimum Mg/ATP-ratio: 1-2, inhibits at higher values Klebsiella aerogenes
2.7.1.47 Mn2+ activation, can replace Mg2+ with 54% efficiency Klebsiella aerogenes

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.47 ATP + D-ribulose Klebsiella aerogenes inducible enzyme of ribitol catabolism ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.47 Klebsiella aerogenes
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.47 partial Klebsiella aerogenes

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.47 additional information
-
-
 Klebsiella aerogenes

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.47 ATP + D-ribulose specific for D-isomer Klebsiella aerogenes ADP + D-ribulose 5-phosphate
-
 ?
2.7.1.47 ATP + D-ribulose ATP cannot be replaced by ITP, GTP or ADP Klebsiella aerogenes ADP + D-ribulose 5-phosphate
-
 ?
2.7.1.47 ATP + D-ribulose inducible enzyme of ribitol catabolism Klebsiella aerogenes ?
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.47 7 9.5 broad Klebsiella aerogenes

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.7.1.47 6 10.5 pH 6.0: about 70% of maximal activity, pH 10.5: about half-maximal activity at pH 10.5 Klebsiella aerogenes