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Literature summary extracted from

  • Thompson, J.; Sackett, D.L.; Donkersloot, J.A.
    Purification and properties of fructokinase I from Lactococcus lactis. Localization of scrK on the sucrose-nisin transposon Tn5306 (1991), J. Biol. Chem., 266, 22626-22633.
    View publication on PubMed

General Stability

EC Number General Stability Organism
2.7.1.4 no significant loss of activity after lyophilization and reconstitution of the enzyme Lactococcus lactis
2.7.1.4 stable after repeated freezing, at -20°C, and thawing Lactococcus lactis

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.4 EDTA
-
 Lactococcus lactis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.4 0.31
-
 D-fructose pH 7.5, 22°C Lactococcus lactis
2.7.1.4 0.59
-
 ATP
-
 Lactococcus lactis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.4 Cd2+ can partially replace Mg2+ in activation Lactococcus lactis
2.7.1.4 Co2+ can partially replace Mg2+ in activation Lactococcus lactis
2.7.1.4 Fe2+ can partially replace Mg2+ in activation Lactococcus lactis
2.7.1.4 Mg2+ Km: 0.53 mM Lactococcus lactis
2.7.1.4 Mg2+ obligate requirement for divalent metal ion, maximal activity with 10 mM Mg2+, other cations can partially replace Mg2+: Co2+, Fe2+, Mn2+, Ni2+, Zn2+, Cd2+ Lactococcus lactis
2.7.1.4 Mn2+
-
 Lactococcus lactis
2.7.1.4 additional information no activation by Ca2+ Lactococcus lactis
2.7.1.4 additional information no activation by Cu2+ Lactococcus lactis
2.7.1.4 additional information no activation by Ba2+ Lactococcus lactis
2.7.1.4 additional information no activation by Sr2+ Lactococcus lactis
2.7.1.4 Ni2+ can partially replace Mg2+ in activation Lactococcus lactis
2.7.1.4 Zn2+ can partially replace Mg2+ in activation Lactococcus lactis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.4 32000
-
 2 * 32000, SDS-PAGE after complete reduction with 2-mercaptoethanol Lactococcus lactis
2.7.1.4 44000
-
 gel filtration Lactococcus lactis
2.7.1.4 60000
-
 nondenaturing equilibrium ultracentrifugation Lactococcus lactis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.4 ATP + D-fructose Lactococcus lactis
-
 ADP + D-fructose 6-phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.4 Lactococcus lactis
-
 fructokinase I: induced specifically by growth on sucrose
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.4
-
 Lactococcus lactis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.4 112
-
-
 Lactococcus lactis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.4 ATP + D-fructose
-
 Lactococcus lactis ADP + D-fructose 6-phosphate
-
 ?
2.7.1.4 ATP + D-mannose
-
 Lactococcus lactis ADP + D-mannose 6-phosphate
-
 ?
2.7.1.4 dATP + D-fructose
-
 Lactococcus lactis dADP + D-fructose 6-phosphate
-
 ?
2.7.1.4 GTP + D-fructose
-
 Lactococcus lactis GDP + D-fructose 6-phosphate
-
 ?
2.7.1.4 ITP + D-fructose
-
 Lactococcus lactis IDP + D-fructose 6-phosphate
-
 ?
2.7.1.4 additional information very low activity as phosphate donors: dGTP, TTP, XTP Lactococcus lactis ?
-
 ?
2.7.1.4 UTP + D-fructose
-
 Lactococcus lactis UDP + D-fructose 6-phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.1.4 dimer 2 * 32000, SDS-PAGE after complete reduction with 2-mercaptoethanol Lactococcus lactis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.4 7 8
-
 Lactococcus lactis

pI Value

EC Number Organism Comment pI Value Maximum pI Value
2.7.1.4 Lactococcus lactis polypeptide 1, pre-cast Ampholine PAGplates at 10°C 5.9 5.6