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Literature summary extracted from

  • Lee, M.; Leustek, T.
    Identification of the gene encoding homoserine kinase from Arabidopsis thaliana and characterization of the recombinant enzyme derived from the gene (1999), Arch. Biochem. Biophys., 372, 135-142.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.39 expression of a His-tagged construct in Escherichia coli Arabidopsis thaliana

General Stability

EC Number General Stability Organism
2.7.1.39 enzyme stored with 3 mM ATP loses 50% of its activity after two freezing cycles. In presence of 50% v/v glycerol the enzyme loses 50% of its activity after 10 cycles. In the basic storage buffer, the enzyme loses 80% of its activity after a single freeze-thaw cycle Arabidopsis thaliana

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.39 0.32
-
 ATP
-
 Arabidopsis thaliana
2.7.1.39 0.4
-
 L-homoserine
-
 Arabidopsis thaliana

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.39 K+ requires 40 mM K+ Arabidopsis thaliana
2.7.1.39 Mn2+ requires 3 mM Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.39 ATP + L-homoserine Arabidopsis thaliana formation of O-phospho-L-homoserine, a branch point intermediate in the pathways for Met and Thr in plants ADP + O-phospho-L-homoserine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.39 Arabidopsis thaliana Q8L7R2
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.39 3.09
-
 L-homoserine-dependent ADP synthesis assay at pH 8.5 and 37°C Arabidopsis thaliana

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.39 ATP + L-homoserine
-
 Arabidopsis thaliana ADP + O-phospho-L-homoserine
-
 ?
2.7.1.39 ATP + L-homoserine formation of O-phospho-L-homoserine, a branch point intermediate in the pathways for Met and Thr in plants Arabidopsis thaliana ADP + O-phospho-L-homoserine
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.1.39 47
-
-
 Arabidopsis thaliana

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.39 8
-
-
 Arabidopsis thaliana