Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Burr, B.; Walker, J.; Truffa-Bachi, P.; Cohen, G.N.
    Homoserine kinase from Escherichia coli K12 (1976), Eur. J. Biochem., 62, 519-526.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.39 2-chloro-L-alanine
-
 Escherichia coli
2.7.1.39 L-2-aminobutyrate
-
 Escherichia coli
2.7.1.39 L-Cys
-
 Escherichia coli
2.7.1.39 L-homocysteine
-
 Escherichia coli
2.7.1.39 L-Ile
-
 Escherichia coli
2.7.1.39 L-Met
-
 Escherichia coli
2.7.1.39 L-Ser
-
 Escherichia coli
2.7.1.39 L-Thr competitive to the substrate L-homoserine Escherichia coli
2.7.1.39 L-Val
-
 Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.39 0.24
-
 L-homoserine pH 7.8, 37°C Escherichia coli
2.7.1.39 0.55
-
 ATP pH 7.8, 37°C Escherichia coli

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.39 Mn2+ high requirement Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.39 29000
-
 2 * 29000, SDS-PAGE Escherichia coli
2.7.1.39 60000
-
 gel filtration Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.39 Escherichia coli
-
 K-12
-
2.7.1.39 Escherichia coli
-
 derepressed strain
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.39
-
 Escherichia coli

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.39 additional information
-
-
 Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.39 ATP + L-homoserine
-
 Escherichia coli ADP + O-phospho-L-homoserine
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.1.39 dimer 2 * 29000, SDS-PAGE Escherichia coli

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.7.1.39 0.4
-
 L-2-aminobutyrate pH 7.8, 37°C Escherichia coli
2.7.1.39 0.6
-
 L-Thr pH 7.8, 37°C Escherichia coli
2.7.1.39 1
-
 L-Cys pH 7.8, 37°C Escherichia coli
2.7.1.39 4
-
 L-homocysteine pH 7.8, 37°C Escherichia coli
2.7.1.39 4.5
-
 L-Ile pH 7.8, 37°C Escherichia coli
2.7.1.39 10
-
 L-Val pH 7.8, 37°C Escherichia coli
2.7.1.39 15
-
 2-chloro-L-alanine pH 7.8, 37°C Escherichia coli
2.7.1.39 27
-
 L-Ser pH 7.8, 37°C Escherichia coli
2.7.1.39 35
-
 L-Met pH 7.8, 37°C Escherichia coli