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Literature summary extracted from

  • Shames, S.L.; Wedler, F.C.
    Homoserine kinase of Escherichia coli: kinetic mechanism and inhibition by L-aspartate semialdehyde (1984), Arch. Biochem. Biophys., 235, 359-370.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.39 5'-adenylylimidodiphosphate
-
 Escherichia coli
2.7.1.39 L-alpha-aminobutyric acid
-
 Escherichia coli
2.7.1.39 L-aspartate semialdehyde mixed inhibition versus L-homoserine and ATP Escherichia coli
2.7.1.39 L-homoserine above 0.75 mM Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.39 0.15
-
 L-homoserine pH 7.8 Escherichia coli
2.7.1.39 0.58
-
 L-aspartate semialdehyde pH 7.8 Escherichia coli
2.7.1.39 3.8
-
 4-hydroxynorvaline pH 7.8 Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.39 Escherichia coli
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.39
-
 Escherichia coli

Reaction

EC Number Reaction Comment Organism Reaction ID
2.7.1.39 ATP + L-homoserine = ADP + O-phospho-L-homoserine ordered random mechanism with ATP preferentially binding before L-homoserine Escherichia coli
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.39 ATP + 4-hydroxynorvaline 0.3% of the activity with L-homoserine Escherichia coli ADP + 4-phospho-L-norvaline
-
 ?
2.7.1.39 ATP + L-aspartate 4-semialdehyde 10% of the activity with L-homoserine Escherichia coli ?
-
 ?
2.7.1.39 ATP + L-homoserine
-
 Escherichia coli ADP + O-phospho-L-homoserine
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.7.1.39 50
-
 stable up to in absence of ligands Escherichia coli

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.7.1.39 0.15
-
 L-alpha-aminobutyric acid
-
 Escherichia coli
2.7.1.39 0.9
-
 5'-adenylylimidodiphosphate pH 7.4 Escherichia coli
2.7.1.39 2
-
 L-homoserine pH 7.6 Escherichia coli