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Homoserine kinase of Escherichia coli: kinetic mechanism and inhibition by L-aspartate semialdehyde

Shames, S.L.; Wedler, F.C.; Arch. Biochem. Biophys. 235, 359-370 (1984)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
2.7.1.39
5'-adenylylimidodiphosphate
-
Escherichia coli
2.7.1.39
L-alpha-aminobutyric acid
-
Escherichia coli
2.7.1.39
L-aspartate semialdehyde
mixed inhibition versus L-homoserine and ATP
Escherichia coli
2.7.1.39
L-homoserine
above 0.75 mM
Escherichia coli
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.7.1.39
0.15
-
L-homoserine
pH 7.8
Escherichia coli
2.7.1.39
0.58
-
L-aspartate semialdehyde
pH 7.8
Escherichia coli
2.7.1.39
3.8
-
4-hydroxynorvaline
pH 7.8
Escherichia coli
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.7.1.39
Escherichia coli
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
2.7.1.39
-
Escherichia coli
Reaction
EC Number
Reaction
Commentary
Organism
2.7.1.39
ATP + L-homoserine = ADP + O-phospho-L-homoserine
ordered random mechanism with ATP preferentially binding before L-homoserine
Escherichia coli
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.1.39
ATP + 4-hydroxynorvaline
0.3% of the activity with L-homoserine
641463
Escherichia coli
ADP + 4-phospho-L-norvaline
-
-
-
?
2.7.1.39
ATP + L-aspartate 4-semialdehyde
10% of the activity with L-homoserine
641463
Escherichia coli
?
-
-
-
?
2.7.1.39
ATP + L-homoserine
-
641463
Escherichia coli
ADP + O-phospho-L-homoserine
-
641463
Escherichia coli
?
Temperature Stability [°C]
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
2.7.1.39
50
-
stable up to in absence of ligands
Escherichia coli
Ki Value [mM]
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
2.7.1.39
0.15
-
L-alpha-aminobutyric acid
-
Escherichia coli
2.7.1.39
0.9
-
5'-adenylylimidodiphosphate
pH 7.4
Escherichia coli
2.7.1.39
2
-
L-homoserine
pH 7.6
Escherichia coli
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
2.7.1.39
5'-adenylylimidodiphosphate
-
Escherichia coli
2.7.1.39
L-alpha-aminobutyric acid
-
Escherichia coli
2.7.1.39
L-aspartate semialdehyde
mixed inhibition versus L-homoserine and ATP
Escherichia coli
2.7.1.39
L-homoserine
above 0.75 mM
Escherichia coli
Ki Value [mM] (protein specific)
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
2.7.1.39
0.15
-
L-alpha-aminobutyric acid
-
Escherichia coli
2.7.1.39
0.9
-
5'-adenylylimidodiphosphate
pH 7.4
Escherichia coli
2.7.1.39
2
-
L-homoserine
pH 7.6
Escherichia coli
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.7.1.39
0.15
-
L-homoserine
pH 7.8
Escherichia coli
2.7.1.39
0.58
-
L-aspartate semialdehyde
pH 7.8
Escherichia coli
2.7.1.39
3.8
-
4-hydroxynorvaline
pH 7.8
Escherichia coli
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.7.1.39
-
Escherichia coli
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.1.39
ATP + 4-hydroxynorvaline
0.3% of the activity with L-homoserine
641463
Escherichia coli
ADP + 4-phospho-L-norvaline
-
-
-
?
2.7.1.39
ATP + L-aspartate 4-semialdehyde
10% of the activity with L-homoserine
641463
Escherichia coli
?
-
-
-
?
2.7.1.39
ATP + L-homoserine
-
641463
Escherichia coli
ADP + O-phospho-L-homoserine
-
641463
Escherichia coli
?
Temperature Stability [°C] (protein specific)
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
2.7.1.39
50
-
stable up to in absence of ligands
Escherichia coli