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Literature summary extracted from

  • Chu, X.; Li, D.
    Cloning, expression, and purification of His-tagged rat mevalonate kinase (2003), Protein Expr. Purif., 27, 165-170.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.36 expression of His-tagged mevalonate kinase in Escherichia coli Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.36 0.035
-
 (R,S)-mevalonate pH 7.5, 25°C, recombinant mevalonate kinase Rattus norvegicus
2.7.1.36 0.95
-
 ATP pH 7.5, 25°C, recombinant mevalonate kinase Rattus norvegicus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.36 42000
-
 2 * 42000, recombinant mevalonate kinase, SDS-PAGE Rattus norvegicus
2.7.1.36 84000
-
 recombinant mevalonate kinase, gel filtration Rattus norvegicus

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.36 Rattus norvegicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.36 recombinant His-tagged mevalonate kinase, hiTrap column Rattus norvegicus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.36 32.7
-
 recombinant mevalonate kinase Rattus norvegicus

Storage Stability

EC Number Storage Stability Organism
2.7.1.36 4°C, 20 mM potassium phosphate, pH 7.5, 5% glycerol, 5 mM 2-mercaptoethanol, 1 month, no loss of activity Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.36 ATP + mevalonate
-
 Rattus norvegicus ADP + phosphomevalonate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.1.36 dimer 2 * 42000, recombinant mevalonate kinase, SDS-PAGE Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.36 7 8 recombinant mevalonate kinase Rattus norvegicus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.7.1.36 6 9 at least 80% of maximal activity over the whole range, recombinant mevalonate kinase Rattus norvegicus