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Literature summary extracted from

  • Suarez, D.; Garcia-Peregrin, E.
    Properties and partial purification of mevalonate kinase from Agave americana (1977), Phytochemistry, 16, 661-665.
No PubMed abstract available

General Stability

EC Number General Stability Organism
2.7.1.36 sensitive to dialysis Agave americana

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.36 Mn2+ inhibition at high concentrations Agave americana

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.36 0.05
-
 mevalonate pH 7.9, 37°C Agave americana
2.7.1.36 0.14
-
 ATP pH 7.9, 37°C Agave americana

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.36 Mg2+ maximal activity at 6-8 mM Agave americana
2.7.1.36 Mn2+ maximal acitvation at 2 mM, inhibition above Agave americana

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.36 Agave americana
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.36 ammonium sulfate, Sephadex G-100, DEAE-Sephadex Agave americana

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.1.36 flower
-
 Agave americana
-
2.7.1.36 leaf
-
 Agave americana
-
2.7.1.36 scape
-
 Agave americana
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.36 additional information
-
 0.58 dpm/mg Agave americana

Storage Stability

EC Number Storage Stability Organism
2.7.1.36 5°C, stable for several months in buffer containing dithiothreitol Agave americana

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.36 ATP + mevalonate ATP is the most effective phosphate donor Agave americana ADP + phosphomevalonate
-
 ?
2.7.1.36 GTP + mevalonate
-
 Agave americana GDP + phosphomevalonate
-
 ?
2.7.1.36 ITP + mevalonate
-
 Agave americana IDP + phosphomevalonate
-
 ?
2.7.1.36 UTP + mevalonate
-
 Agave americana UDP + phosphomevalonate
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.36 7.9
-
-
 Agave americana