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Literature summary extracted from

  • Gray, J.C.; Kekwick, R.G.O.
    Mevalonate kinase in green leaves and etiolated cotyledons of the french bean Phaseolus vulgaris (1973), Biochem. J., 133, 335-347.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.1.36 iodoacetamide 10 mM, 20% activation Phaseolus vulgaris

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.36 ADP very weak inhibition Phaseolus vulgaris
2.7.1.36 CuSO4 10 mM, almost complete inhibition Phaseolus vulgaris
2.7.1.36 farnesyl diphosphate 0.3 mM, approx. 75% inhibition Phaseolus vulgaris
2.7.1.36 geranyl diphosphate 0.3 mM, approx. 10% inhibition Phaseolus vulgaris
2.7.1.36 geranylgeranyl diphosphate 0.3 mM, approx. 90% inhibition Phaseolus vulgaris
2.7.1.36 mevalonate at high concentrations Phaseolus vulgaris
2.7.1.36 mevalonate 5-phosphate 0.3 mM, approx. 45% inhibition Phaseolus vulgaris
2.7.1.36 Mn2+ inhibition at high concentrations Phaseolus vulgaris
2.7.1.36 p-chloromercuribenzoate 1 mM, almost complete inhibition Phaseolus vulgaris
2.7.1.36 phytyl diphosphate 0.3 mM, almost complete inhibition Phaseolus vulgaris

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.36 0.0426
-
 RS-mevalonate pH 7.0, 30°C, enzyme from etiolated cotyledons Phaseolus vulgaris
2.7.1.36 0.046
-
 RS-mevalonate pH 7.0, 30°C, leaf enzyme Phaseolus vulgaris
2.7.1.36 1.54
-
 ATP pH 7.0, 30°C, enzyme from etiolated cotyledons Phaseolus vulgaris
2.7.1.36 1.75
-
 ATP pH 7.0, 30°C, enzyme fom green leaves Phaseolus vulgaris
2.7.1.36 2
-
 ATP pH 7.0, 30°C Phaseolus vulgaris

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.36 Mg2+ maximal activity at 9 mM Phaseolus vulgaris
2.7.1.36 Mn2+ most active at low concentrations Phaseolus vulgaris
2.7.1.36 Mn2+ maximal activation at 2 mM Phaseolus vulgaris

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.36 97000 104000 gel filtration Phaseolus vulgaris

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.36 Phaseolus vulgaris
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.36 enzyme from cotyledons and leaves, ammonium sulfate, Sephadex G-200, DEAE-Sephadex Phaseolus vulgaris

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.1.36 cotyledon etiolated Phaseolus vulgaris
-
2.7.1.36 leaf
-
 Phaseolus vulgaris
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.36 ADP + mevalonate
-
 Phaseolus vulgaris ?
-
 ?
2.7.1.36 ATP + mevalonate
-
 Phaseolus vulgaris ADP + phosphomevalonate
-
 ?
2.7.1.36 CTP + mevalonate weak activity Phaseolus vulgaris CDP + phosphomevalonate
-
 ?
2.7.1.36 GTP + mevalonate weak activity Phaseolus vulgaris GDP + phosphomevalonate
-
 ?
2.7.1.36 ITP + mevalonate
-
 Phaseolus vulgaris IDP + phosphomevalonate
-
 ?
2.7.1.36 UTP + mevalonate
-
 Phaseolus vulgaris UDP + phosphomevalonate
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.36 6.5 7.5
-
 Phaseolus vulgaris

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.7.1.36 5.5 9 approx. 30% of maximal activity at pH 5.5, approx. 60% of maximal activity at pH 9 Phaseolus vulgaris