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Literature summary extracted from

  • Tchen, T.T.
    Mevalonic kinase: purification and properties (1958), J. Biol. Chem., 233, 1100-1103.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.1.36 UTP stimulation of ATP utilization Saccharomyces cerevisiae

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.36 p-chloromercuribenzoate 0.1 mM, 83% inhibition, completely reversed by reduced glutathione Saccharomyces cerevisiae

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.36 Ca2+ can partially replace Mn2+ in activation Saccharomyces cerevisiae
2.7.1.36 Ca2+ at 5 mM 10% of the activity with Mn2+ Saccharomyces cerevisiae
2.7.1.36 Co2+ can partially replace Mn2+ in activation Saccharomyces cerevisiae
2.7.1.36 Co2+ approx. 15% of the activity with Mn2+ at 5 mM Saccharomyces cerevisiae
2.7.1.36 Fe2+ can partially replace Mn2+ in activation Saccharomyces cerevisiae
2.7.1.36 Fe2+ at 5 mM about 10% of the activity with Mn2+ Saccharomyces cerevisiae
2.7.1.36 Mg2+ Mg2+ becomes increasingly active at higher concentrations and at sufficiently high concentrations the most active metal ion Saccharomyces cerevisiae
2.7.1.36 Mn2+ divalent cation required for activity Saccharomyces cerevisiae
2.7.1.36 Mn2+ higher activation than with Mg2+ at concentrations below approx. 0.008 mM, lower activation than with Mg2+ above Saccharomyces cerevisiae
2.7.1.36 Zn2+ can partially replace Mn2+ in activation Saccharomyces cerevisiae
2.7.1.36 Zn2+ at 5 mM about 30% of the activity of Mn2+ Saccharomyces cerevisiae

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.36 Saccharomyces cerevisiae
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.36 protamine sulfate, ammonium sulfate, acid treatment at pH 5.0, ammonium sulfate Saccharomyces cerevisiae

Storage Stability

EC Number Storage Stability Organism
2.7.1.36 -10°C, several months, no loss of activity Saccharomyces cerevisiae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.36 ATP + mevalonate no activity with ADP Saccharomyces cerevisiae ADP + phosphomevalonate
-
 ?
2.7.1.36 CTP + mevalonate
-
 Saccharomyces cerevisiae CDP + phosphomevalonate
-
 ?
2.7.1.36 GTP + mevalonate
-
 Saccharomyces cerevisiae GDP + phosphomevalonate
-
 ?
2.7.1.36 UTP + mevalonate
-
 Saccharomyces cerevisiae UDP + phosphomevalonate
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.36 6.4 6.7
-
 Saccharomyces cerevisiae

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.7.1.36 5 9 more than 50% of maximal activity at pH 5.0 and pH 9.0 Saccharomyces cerevisiae