Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Shimizu, S.; Kubo, K.; Tani, Y.; Ogata, K.
    Purification and properties of pantothenate kinase from brevibacterium ammoniagenes IFO 12071 (1973), Agric. Biol. Chem., 37, 2863-2870.
No PubMed abstract available

General Stability

EC Number General Stability Organism
2.7.1.33 ATP and/or sucrose do not stabilize Corynebacterium ammoniagenes

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.33 ADP not Corynebacterium ammoniagenes
2.7.1.33 AlCl3 strong Corynebacterium ammoniagenes
2.7.1.33 Ba2+
-
 Corynebacterium ammoniagenes
2.7.1.33 CoA
-
 Corynebacterium ammoniagenes
2.7.1.33 Cu2+ strong Corynebacterium ammoniagenes
2.7.1.33 D-pantothenate substrate inhibition, above 0.5 mM Corynebacterium ammoniagenes
2.7.1.33 dephospho-CoA
-
 Corynebacterium ammoniagenes
2.7.1.33 EDTA
-
 Corynebacterium ammoniagenes
2.7.1.33 Fe2+ strong Corynebacterium ammoniagenes
2.7.1.33 HgCl2 strong Corynebacterium ammoniagenes
2.7.1.33 additional information no inhibition by 2,2'-dipyridyl, Ca2+, Cd2+, 3'-AMP, GTP, GDP, ITP, UTP; not inhibitory: 3',5'-ADP Corynebacterium ammoniagenes
2.7.1.33 Na2HAsO4
-
 Corynebacterium ammoniagenes
2.7.1.33 NH4+
-
 Corynebacterium ammoniagenes
2.7.1.33 pantetheine
-
 Corynebacterium ammoniagenes
2.7.1.33 pantetheine 4'-phosphate weak Corynebacterium ammoniagenes
2.7.1.33 pantothenic acid 4'-phosphate
-
 Corynebacterium ammoniagenes
2.7.1.33 pantothenoylcysteine 4'-phosphate weak Corynebacterium ammoniagenes
2.7.1.33 pantothenyl alcohol
-
 Corynebacterium ammoniagenes
2.7.1.33 Pb2+
-
 Corynebacterium ammoniagenes

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.33 0.035
-
 ATP pH 6.5, 37°C Corynebacterium ammoniagenes
2.7.1.33 0.067
-
 pantothenate pH 6.5, 37°C Corynebacterium ammoniagenes
2.7.1.33 1
-
 ATP pH 6.5, 37°C Corynebacterium ammoniagenes
2.7.1.33 1
-
 ATP ATP in form of MgATP2- Corynebacterium ammoniagenes

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.33 Co2+ activation, can replace Mg2+ Corynebacterium ammoniagenes
2.7.1.33 Mg2+ Km-value: 1 mM Corynebacterium ammoniagenes
2.7.1.33 Mg2+ requirement, active substrate: MgATP-complex Corynebacterium ammoniagenes
2.7.1.33 Mn2+ activation, can replace Mg2+ Corynebacterium ammoniagenes
2.7.1.33 additional information no activation by Ca2+, Cd2+, Ba2+, Pb2+, Fe2+ or Cu2+ Corynebacterium ammoniagenes
2.7.1.33 Ni2+ activation, can replace Mg2+ with about 50% efficiency Corynebacterium ammoniagenes
2.7.1.33 Zn2+ activation, can replace Mg2+ with about 50% efficiency Corynebacterium ammoniagenes

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.33 45000
-
 gel filtration Corynebacterium ammoniagenes

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.33 Corynebacterium ammoniagenes
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.33
-
 Corynebacterium ammoniagenes

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.33 0.034
-
 pH 6.5, 37°C Corynebacterium ammoniagenes

Storage Stability

EC Number Storage Stability Organism
2.7.1.33 4°C, 2 mg protein/ml in 0.01 M phosphate buffer, pH 7, inactivation within 4 days Corynebacterium ammoniagenes

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.33 ATP + (R)-pantothenate
-
 Corynebacterium ammoniagenes ADP + (R)-4'-phosphopantothenate
-
 ?
2.7.1.33 ATP + D-pantothenate
-
 Corynebacterium ammoniagenes ADP + D-4'-phosphopantothenate
-
 ?
2.7.1.33 GTP + pantothenate phosphorylation at 28% the rate of ATP Corynebacterium ammoniagenes GDP + D-4'-phosphopantothenate
-
 ?
2.7.1.33 ITP + pantothenate phosphorylation at 46% the rate of ATP Corynebacterium ammoniagenes IDP + D-4'-phosphopantothenate
-
 ?
2.7.1.33 pantetheine + ATP
-
 Corynebacterium ammoniagenes D-4'-phosphopantetheine + ADP
-
 ?
2.7.1.33 panthenoylcysteine + ATP
-
 Corynebacterium ammoniagenes D-4'-phosphopanthenoylcysteine + ADP
-
 ?
2.7.1.33 pantothenyl alcohol + ATP
-
 Corynebacterium ammoniagenes D-4'-phosphopantothenyl alcohol + ADP
-
 ?
2.7.1.33 UTP + pantothenate phosphorylation at 18% the rate of ATP Corynebacterium ammoniagenes UDP + D-4'-phosphopantothenate
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.1.33 30 37
-
 Corynebacterium ammoniagenes

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.7.1.33 30
-
 10% to 20% loss of activity within 10 min at pH 7.5 to 8, 10 min stable at pH 7, t1/2: 10 min at pH 6.5 Corynebacterium ammoniagenes
2.7.1.33 37
-
 10 min, 70% loss of activity Corynebacterium ammoniagenes
2.7.1.33 43
-
 10 min, inactivation Corynebacterium ammoniagenes

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.33 6.5 7
-
 Corynebacterium ammoniagenes

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
2.7.1.33 6.5
-
 t1/2: 10 min at 30°C Corynebacterium ammoniagenes
2.7.1.33 7
-
 10 min stable at 30°C Corynebacterium ammoniagenes
2.7.1.33 7.5
-
 90% of activity retained after 10 min at 30°C Corynebacterium ammoniagenes
2.7.1.33 8
-
 80% of activity retained after 10 min at 30°C Corynebacterium ammoniagenes

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.7.1.33 0.033
-
 CoA reduced form, pH 6.5, 37°C Corynebacterium ammoniagenes
2.7.1.33 0.037
-
 pantetheine 4'-phosphate pH 6.5, 37°C Corynebacterium ammoniagenes
2.7.1.33 0.067
-
 CoA oxidized form, pH 6.5, 37°C Corynebacterium ammoniagenes
2.7.1.33 0.125
-
 pantothenic acid 4'-phosphate pH 6.5, 37°C Corynebacterium ammoniagenes