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Literature summary extracted from

  • Gee, P.; Kent, C.
    Multiple isoforms of choline kinase from Caenorhabditis elegans: cloning, expression, purification, and characterization (2003), Biochim. Biophys. Acta, 1648, 33-42.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.32 7 different genes, cloned 5 Caenorhabditis elegans

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.32 0.7
-
 ATP isozyme CKB-2, pH 10.0, 37°C Caenorhabditis elegans
2.7.1.32 1.6
-
 choline isozyme CKA-2, pH 10.0, 37°C Caenorhabditis elegans
2.7.1.32 2.4
-
 ATP isozyme CKA-2, pH 10.0, 37°C Caenorhabditis elegans
2.7.1.32 13
-
 choline isozyme CKB-2, pH 10.0, 37°C Caenorhabditis elegans

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.32 Mg2+ required Caenorhabditis elegans
2.7.1.32 Mn2+ poor substitute for Mg2+ Caenorhabditis elegans

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.32 40600
-
 isoform CKB-2: x * 40600, SDS-PAGE, x * 42000, deduced from gene sequence Caenorhabditis elegans
2.7.1.32 42000
-
 isoform CKB-2: x * 40600, SDS-PAGE, x * 42000, deduced from gene sequence Caenorhabditis elegans
2.7.1.32 48600
-
 isoform CKA-2: x * 48600, SDS-PAGE, x * 49000, deduced from gene sequence Caenorhabditis elegans
2.7.1.32 49000
-
 isoform CKA-2: x * 48600, SDS-PAGE, x * 49000, deduced from gene sequence Caenorhabditis elegans

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.32 Caenorhabditis elegans
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.32 isoforms CKA-2, CKB-2 Caenorhabditis elegans

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.32 2.4
-
 isozyme CKB-2, pH 10.0, 37°C Caenorhabditis elegans
2.7.1.32 43
-
 isozyme CKA-2, pH 10.0, 37°C Caenorhabditis elegans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.32 ATP + choline
-
 Caenorhabditis elegans ADP + O-phosphocholine
-
 ?
2.7.1.32 ATP + ethanolamine poor substrate compared to choline Caenorhabditis elegans ADP + ethanolamine phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.1.32 ? isoform CKB-2: x * 40600, SDS-PAGE, x * 42000, deduced from gene sequence Caenorhabditis elegans
2.7.1.32 dimer isoform CKA-2: x * 48600, SDS-PAGE, x * 49000, deduced from gene sequence Caenorhabditis elegans

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.32 10
-
 isoform CKA-2 Caenorhabditis elegans