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Literature summary extracted from

  • Brostrom, M.A.; Browning, E.T.
    Choline kinase from brewers yeast. Partial purification, properties, and kinetic mechanism (1973), J. Biol. Chem., 248, 2364-2371.
    View publication on PubMed

General Stability

EC Number General Stability Organism
2.7.1.32 choline, ATP, bovine serum albumin and changes in ionic strength do not contribute to the stability of choline kinase Saccharomyces cerevisiae
2.7.1.32 loss of activity upon freezing and thawing Saccharomyces cerevisiae

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.32 5,5'-dithiobis(2-nitrobenzoic acid)
-
 Saccharomyces cerevisiae
2.7.1.32 Acetylcholine
-
 Saccharomyces cerevisiae
2.7.1.32 ADP
-
 Saccharomyces cerevisiae
2.7.1.32 N-ethylmaleimide
-
 Saccharomyces cerevisiae
2.7.1.32 phosphorylcholine
-
 Saccharomyces cerevisiae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.32 additional information
-
 additional information
-
 Saccharomyces cerevisiae
2.7.1.32 0.14
-
 ATP pH 8.5, 25°C Saccharomyces cerevisiae

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.32 67000
-
 gel filtration Saccharomyces cerevisiae

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.32 Saccharomyces cerevisiae
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.32
-
 Saccharomyces cerevisiae

Reaction

EC Number Reaction Comment Organism Reaction ID
2.7.1.32 ATP + choline = ADP + phosphocholine random bi-bi mechanism Saccharomyces cerevisiae
a

Storage Stability

EC Number Storage Stability Organism
2.7.1.32 0°C, 10 mM Tris actetate, pH 7.2, 13 mM 2-mercaptoethanol, 1 mM EGTA, 15 mM MgCl2, stable for 1 week Saccharomyces cerevisiae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.32 ATP + choline
-
 Saccharomyces cerevisiae ADP + O-phosphocholine
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.1.32 25
-
 assay at Saccharomyces cerevisiae

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.7.1.32 30
-
 40 min, without Mg2+, 80% loss of activity, + 15 mM Mg2+, 9% loss of activity Saccharomyces cerevisiae
2.7.1.32 39
-
 10 min, 15 mM Mg2+, 50% loss of activity Saccharomyces cerevisiae

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
2.7.1.32 5
-
 or below, 2-3 h, 90% or more loss of activity, crude enzyme extract Saccharomyces cerevisiae