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Literature summary extracted from

  • Thorner, J.W.
    Glycerol kinase (1975), Methods Enzymol., 42C, 148-156.
No PubMed abstract available

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.7.1.30
-
 Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.30 ADP
-
 Escherichia coli
2.7.1.30 fructose 1,6-diphosphate
-
 Escherichia coli
2.7.1.30 p-Hydroxymercuriphenylsulfonate
-
 Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.30 additional information
-
 additional information assay at 25°C, increasing pH or decreasing temperature rises Km for glycerol Escherichia coli
2.7.1.30 0.5
-
 dihydroxyacetone pH 7.0, 25°C Escherichia coli

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.30 KCl 0.04 M, stimulates 30% at neutral pH Escherichia coli
2.7.1.30 Mg2+ true substrate: MgATP-complex Escherichia coli
2.7.1.30 Mn2+ can substitute for Mg2+ Escherichia coli
2.7.1.30 Mn2+ rate is 3-fold lower than with Mg2+ Escherichia coli
2.7.1.30 additional information no effect: Ca2+ Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.30 55000
-
 4 * 55000, SDS-PAGE Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.30 Escherichia coli
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.30
-
 Escherichia coli

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.30 41.2
-
 pH 7.0, 25°C Escherichia coli

Storage Stability

EC Number Storage Stability Organism
2.7.1.30 0°C, suspension of crystals, 10 mM glycerol, 1 mM EDTA, 1 mM 2-mercaptoethanol, 0.1 M potassium phosphate, pH 7.0, saturated with ammonium sulfate, stable for several years Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.30 ATP + D-glyceraldehyde
-
 Escherichia coli ADP + D-glyceraldehyde 3-phosphate
-
 ?
2.7.1.30 ATP + dihydroxyacetone
-
 Escherichia coli ADP + dihydroxyacetone phosphate
-
 ?
2.7.1.30 ATP + glycerol high specificity for ATP, no other ribonucleoside triphosphate utilized Escherichia coli ADP + sn-glycerol 3-phosphate
-
 ir
2.7.1.30 ATP + L-glyceraldehyde
-
 Escherichia coli ADP + L-glyceraldehyde 3-phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.1.30 tetramer 4 * 55000, SDS-PAGE Escherichia coli

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.1.30 25
-
 assay at Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.30 9.5
-
-
 Escherichia coli

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.7.1.30 7 9.5 pH 7.0: about 50% of maximum activity, pH 9.5: maximum activity Escherichia coli