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Literature summary extracted from

  • Raushel, F.M.; Cleland, W.W.
    Bovine liver fructokinase: purification and kinetic properties (1977), Biochemistry, 16, 2169-2175.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.3 1-deoxy-D-fructose dead-end inhibitor Bos taurus
2.7.1.3 2,5-anhydro-D-glucitol
-
 Bos taurus
2.7.1.3 2,5-anhydro-D-lyxitol
-
 Bos taurus
2.7.1.3 2,5-anhydro-D-mannitol
-
 Bos taurus
2.7.1.3 2,5-anhydro-D-mannose
-
 Bos taurus
2.7.1.3 5-Thio-D-fructose competitive to D-fructose Bos taurus
2.7.1.3 CrADP dead-end inhibitor Bos taurus
2.7.1.3 CrATP dead-end inhibitor Bos taurus
2.7.1.3 D-Fructose 1-phosphate competitive against D-fructose, noncompetitive to MgATP2- Bos taurus
2.7.1.3 D-mannoheptulose
-
 Bos taurus
2.7.1.3 D-psicose
-
 Bos taurus
2.7.1.3 D-ribono-gamma-lactone
-
 Bos taurus
2.7.1.3 D-ribose
-
 Bos taurus
2.7.1.3 D-sedoheptulose
-
 Bos taurus
2.7.1.3 D-tagatose
-
 Bos taurus
2.7.1.3 D-xylulose
-
 Bos taurus
2.7.1.3 L-galactoheptulose
-
 Bos taurus
2.7.1.3 L-ribulose
-
 Bos taurus
2.7.1.3 L-sorbose
-
 Bos taurus
2.7.1.3 MgADP- competitive to MgATP2- Bos taurus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.3 0.1
-
 D-fructose 25°C, pH 7 Bos taurus
2.7.1.3 0.37
-
 L-ribulose 25°C, pH 7 Bos taurus
2.7.1.3 0.43
-
 L-sorbose 25°C, pH 7 Bos taurus
2.7.1.3 0.9
-
 D-tagatose 25°C, pH 7 Bos taurus
2.7.1.3 1
-
 2,5-anhydro-D-mannose 25°C, pH 7 Bos taurus
2.7.1.3 1.2
-
 5-keto-D-fructose 25°C, pH 7 Bos taurus
2.7.1.3 1.4
-
 D-xylose 25°C, pH 7 Bos taurus
2.7.1.3 1.6
-
 2,5-anhydro-D-mannitol 25°C, pH 7 Bos taurus
2.7.1.3 1.8
-
 L-galacto-2-heptulose 25°C, pH 7, D-fructose 1-phosphate Bos taurus
2.7.1.3 2.2
-
 D-sedoheptulose 25°C, pH 7 Bos taurus
2.7.1.3 2.9
-
 2,5-anhydro-D-glucitol 25°C, pH 7 Bos taurus
2.7.1.3 10
-
 2,5-anhydro-D-lyxitol 25°C, pH 7 Bos taurus
2.7.1.3 11
-
 D-psicose 25°C, pH 7 Bos taurus
2.7.1.3 58
-
 D-ribono-gamma-lactone 25°C, pH 7 Bos taurus
2.7.1.3 120
-
 D-mannoheptulose 25°C, pH 7 Bos taurus
2.7.1.3 142
-
 D-ribose 25°C, pH 7 Bos taurus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.3 Cs+ activates Bos taurus
2.7.1.3 K+ Km: 15 mM Bos taurus
2.7.1.3 Na+ activates Bos taurus
2.7.1.3 NH4+ activates Bos taurus
2.7.1.3 Rb+ activates Bos taurus
2.7.1.3 Tl+ activates Bos taurus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.3 29000
-
 2 * 29000 SDS-PAGE in the presence of dithiothreitol Bos taurus
2.7.1.3 56000
-
 gel filtration, ultracentrifugation Bos taurus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.3 ATP + D-fructose Bos taurus
-
 ADP + D-fructose 1-phosphate
-
 r

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.3 Bos taurus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.3
-
 Bos taurus

Reaction

EC Number Reaction Comment Organism Reaction ID
2.7.1.3 ATP + D-fructose = ADP + D-fructose 1-phosphate random kinetic mechanism with the formation of two dead-end complexes Bos taurus
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.1.3 liver
-
 Bos taurus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.3 17
-
-
 Bos taurus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.3 ATP + 2,5-anhydro-D-glucitol
-
 Bos taurus ?
-
 r
2.7.1.3 ATP + 2,5-anhydro-D-lyxitol
-
 Bos taurus ?
-
 r
2.7.1.3 ATP + 2,5-anhydro-D-mannitol
-
 Bos taurus ?
-
 r
2.7.1.3 ATP + 2,5-anhydro-D-mannose
-
 Bos taurus ?
-
 r
2.7.1.3 ATP + 5-keto-D-fructose
-
 Bos taurus ?
-
 ?
2.7.1.3 ATP + D-fructose
-
 Bos taurus ADP + D-fructose 1-phosphate
-
 r
2.7.1.3 ATP + D-psicose
-
 Bos taurus ?
-
 r
2.7.1.3 ATP + D-ribono-gamma-lactone
-
 Bos taurus ?
-
 r
2.7.1.3 ATP + D-ribose
-
 Bos taurus ?
-
 r
2.7.1.3 ATP + D-sedoheptulose
-
 Bos taurus ?
-
 r
2.7.1.3 ATP + D-tagatose
-
 Bos taurus ?
-
 r
2.7.1.3 ATP + D-xylose
-
 Bos taurus ADP + D-xylose 1-phosphate
-
 ?
2.7.1.3 ATP + D-xylulose
-
 Bos taurus ?
-
 r
2.7.1.3 ATP + L-galactoheptulose
-
 Bos taurus ?
-
 r
2.7.1.3 ATP + L-ribulose
-
 Bos taurus ?
-
 r
2.7.1.3 ATP + L-sorbose
-
 Bos taurus ?
-
 r
2.7.1.3 ATP + mannoheptulose
-
 Bos taurus ?
-
 r
2.7.1.3 additional information the minimum molecule for substrate activity seems to be (2R)-2-hydroxymethyl-3,4-dihydroxytetrahydrofuran Bos taurus ?
-
 ?
2.7.1.3 additional information not: 5-thio-D-fructose Bos taurus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.1.3 dimer 2 * 29000 SDS-PAGE in the presence of dithiothreitol Bos taurus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.1.3 25
-
 assay at Bos taurus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.3 7
-
 assay at Bos taurus

pI Value

EC Number Organism Comment pI Value Maximum pI Value
2.7.1.3 Bos taurus isoelectric focusing, LKB (pH 3-10) ampholytes were used with 7.5% polyacrylamide gels
-
 5.7