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Literature summary extracted from

  • Schriek, U.; Schwenn, J.D.
    Properties of the purified APS-kinase from Escherichia coli and Saccharomyces cerevisiae (1986), Arch. Microbiol., 145, 32-38.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.1.25 ascorbate activation Escherichia coli
2.7.1.25 dithiothreitol activation Escherichia coli
2.7.1.25 dithiothreitol activation Saccharomyces cerevisiae
2.7.1.25 glutathione reduced glutathione increases the activity significantly Escherichia coli
2.7.1.25 glutathione reduced glutathione increases the activity significantly Saccharomyces cerevisiae
2.7.1.25 L-cysteine activation Escherichia coli
2.7.1.25 additional information not activated by 2-mercaptoethanol Escherichia coli
2.7.1.25 additional information not activated by 2-mercaptoethanol Saccharomyces cerevisiae
2.7.1.25 additional information not activated by L-cysteine and ascorbate Saccharomyces cerevisiae
2.7.1.25 thioredoxin activation in the presence of dithiothreitol Escherichia coli
2.7.1.25 thioredoxin activation in the presence of dithiothreitol Saccharomyces cerevisiae

General Stability

EC Number General Stability Organism
2.7.1.25 rapid loss of activity in dilute solutions Escherichia coli
2.7.1.25 rapid loss of activity in dilute solutions Saccharomyces cerevisiae

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.25 2,4,6-Trinitrobenzene sulfonate 0.05 mM, complete inhibition Escherichia coli
2.7.1.25 2,4,6-Trinitrobenzene sulfonate 0.05 mM, 28% inhibition Saccharomyces cerevisiae
2.7.1.25 2,6-dichlorophenol indophenol 0.05 mM, complete inhibition Escherichia coli
2.7.1.25 2,6-dichlorophenol indophenol 0.05 mM, complete inhibition Saccharomyces cerevisiae
2.7.1.25 Bromosuccinimide 0.05 mM, complete inhibition Escherichia coli
2.7.1.25 Bromosuccinimide 0.05 mM, complete inhibition Saccharomyces cerevisiae
2.7.1.25 dehydroascorbate
-
Escherichia coli
2.7.1.25 dehydroascorbate
-
Saccharomyces cerevisiae
2.7.1.25 FAD
-
Escherichia coli
2.7.1.25 FAD
-
Saccharomyces cerevisiae
2.7.1.25 ferricyanide 0.05 mM, complete inhibition Escherichia coli
2.7.1.25 GSSG
-
Escherichia coli
2.7.1.25 GSSG
-
Saccharomyces cerevisiae
2.7.1.25 Mercuriphenylacetate 0.05 mM, complete inhibition Escherichia coli
2.7.1.25 Mercuriphenylacetate 0.05 mM, complete inhibition Saccharomyces cerevisiae
2.7.1.25 additional information
-
Escherichia coli
2.7.1.25 additional information not inhibited by ferricyanide Saccharomyces cerevisiae
2.7.1.25 oxidized glutathione thioredoxin reverses inactivation Escherichia coli

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.1.25 cytosol
-
Escherichia coli 5829
-
2.7.1.25 cytosol
-
Saccharomyces cerevisiae 5829
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.25 29500
-
in the presence of dithiothreitol, gel filtration Saccharomyces cerevisiae
2.7.1.25 40000 45000 gel filtration Saccharomyces cerevisiae
2.7.1.25 49500 52000 gel filtration Saccharomyces cerevisiae
2.7.1.25 85000 90000 gel filtration Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.25 Escherichia coli
-
-
-
2.7.1.25 Escherichia coli AN1460
-
-
-
2.7.1.25 Saccharomyces cerevisiae
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.25 ammonium sulfate, Blue Sepharose, Sephacryl S-200, PBE 94, Red Sepharose, phenyl-sepharose Saccharomyces cerevisiae
2.7.1.25 ammonium sulfate, Giogel P2, Blue Sepharose, Sephacryl S-300, PBE 94 Escherichia coli

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.25 0.614
-
-
Escherichia coli
2.7.1.25 0.614
-
-
Saccharomyces cerevisiae
2.7.1.25 0.843
-
-
Escherichia coli
2.7.1.25 0.843
-
-
Saccharomyces cerevisiae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.25 ATP + adenosine 5-phosphosulfate i.e. adenylylsulfate or APS Escherichia coli ADP + 3'-phosphoadenosine 5'-phosphosulfate i.e. 3'-phosphoadenylylsulfate or PAPS, via a phosphorylated enzyme intermediate ?
2.7.1.25 ATP + adenosine 5-phosphosulfate i.e. adenylylsulfate or APS Saccharomyces cerevisiae ADP + 3'-phosphoadenosine 5'-phosphosulfate i.e. 3'-phosphoadenylylsulfate or PAPS, via a phosphorylated enzyme intermediate ?
2.7.1.25 ATP + adenosine 5-phosphosulfate i.e. adenylylsulfate or APS Escherichia coli AN1460 ADP + 3'-phosphoadenosine 5'-phosphosulfate i.e. 3'-phosphoadenylylsulfate or PAPS, via a phosphorylated enzyme intermediate ?

Subunits

EC Number Subunits Comment Organism
2.7.1.25 dimer 2 * 28000-29500, dithiothreitol-treated enzyme, SDS-PAGE Saccharomyces cerevisiae
2.7.1.25 dimer 2 * 40000-45000, dithiothreitol-treated enzyme, SDS-PAGE Escherichia coli
2.7.1.25 tetramer monomers and dimers are catalytically active Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.25 additional information
-
pI: 5.5 Escherichia coli
2.7.1.25 additional information
-
pI: 5.5 Saccharomyces cerevisiae
2.7.1.25 additional information
-
pI: 7.4 Escherichia coli
2.7.1.25 additional information
-
pI: 7.4 Saccharomyces cerevisiae
2.7.1.25 6.8 7
-
Escherichia coli
2.7.1.25 6.8 7
-
Saccharomyces cerevisiae
2.7.1.25 8
-
-
Escherichia coli
2.7.1.25 8
-
-
Saccharomyces cerevisiae

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.7.1.25 6 8 continuous increase of activity from pH 6.0 to 8.0 Escherichia coli
2.7.1.25 6 8 continuous increase of activity from pH 6.0 to 8.0 Saccharomyces cerevisiae
2.7.1.25 6.8 8 maximal activity at pH 6.8, approx. half-maximal activity at pH 8.0 Escherichia coli
2.7.1.25 6.8 8 maximal activity at pH 6.8, approx. half-maximal activity at pH 8.0 Saccharomyces cerevisiae