EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
2.7.1.25 | ascorbate | activation | Escherichia coli | |
2.7.1.25 | dithiothreitol | activation | Escherichia coli | |
2.7.1.25 | dithiothreitol | activation | Saccharomyces cerevisiae | |
2.7.1.25 | glutathione | reduced glutathione increases the activity significantly | Escherichia coli | |
2.7.1.25 | glutathione | reduced glutathione increases the activity significantly | Saccharomyces cerevisiae | |
2.7.1.25 | L-cysteine | activation | Escherichia coli | |
2.7.1.25 | additional information | not activated by 2-mercaptoethanol | Escherichia coli | |
2.7.1.25 | additional information | not activated by 2-mercaptoethanol | Saccharomyces cerevisiae | |
2.7.1.25 | additional information | not activated by L-cysteine and ascorbate | Saccharomyces cerevisiae | |
2.7.1.25 | thioredoxin | activation in the presence of dithiothreitol | Escherichia coli | |
2.7.1.25 | thioredoxin | activation in the presence of dithiothreitol | Saccharomyces cerevisiae |
EC Number | General Stability | Organism |
---|---|---|
2.7.1.25 | rapid loss of activity in dilute solutions | Escherichia coli |
2.7.1.25 | rapid loss of activity in dilute solutions | Saccharomyces cerevisiae |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.7.1.25 | 2,4,6-Trinitrobenzene sulfonate | 0.05 mM, complete inhibition | Escherichia coli | |
2.7.1.25 | 2,4,6-Trinitrobenzene sulfonate | 0.05 mM, 28% inhibition | Saccharomyces cerevisiae | |
2.7.1.25 | 2,6-dichlorophenol indophenol | 0.05 mM, complete inhibition | Escherichia coli | |
2.7.1.25 | 2,6-dichlorophenol indophenol | 0.05 mM, complete inhibition | Saccharomyces cerevisiae | |
2.7.1.25 | Bromosuccinimide | 0.05 mM, complete inhibition | Escherichia coli | |
2.7.1.25 | Bromosuccinimide | 0.05 mM, complete inhibition | Saccharomyces cerevisiae | |
2.7.1.25 | dehydroascorbate | - |
Escherichia coli | |
2.7.1.25 | dehydroascorbate | - |
Saccharomyces cerevisiae | |
2.7.1.25 | FAD | - |
Escherichia coli | |
2.7.1.25 | FAD | - |
Saccharomyces cerevisiae | |
2.7.1.25 | ferricyanide | 0.05 mM, complete inhibition | Escherichia coli | |
2.7.1.25 | GSSG | - |
Escherichia coli | |
2.7.1.25 | GSSG | - |
Saccharomyces cerevisiae | |
2.7.1.25 | Mercuriphenylacetate | 0.05 mM, complete inhibition | Escherichia coli | |
2.7.1.25 | Mercuriphenylacetate | 0.05 mM, complete inhibition | Saccharomyces cerevisiae | |
2.7.1.25 | additional information | - |
Escherichia coli | |
2.7.1.25 | additional information | not inhibited by ferricyanide | Saccharomyces cerevisiae | |
2.7.1.25 | oxidized glutathione | thioredoxin reverses inactivation | Escherichia coli |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
2.7.1.25 | cytosol | - |
Escherichia coli | 5829 | - |
2.7.1.25 | cytosol | - |
Saccharomyces cerevisiae | 5829 | - |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.7.1.25 | 29500 | - |
in the presence of dithiothreitol, gel filtration | Saccharomyces cerevisiae |
2.7.1.25 | 40000 | 45000 | gel filtration | Saccharomyces cerevisiae |
2.7.1.25 | 49500 | 52000 | gel filtration | Saccharomyces cerevisiae |
2.7.1.25 | 85000 | 90000 | gel filtration | Escherichia coli |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.7.1.25 | Escherichia coli | - |
- |
- |
2.7.1.25 | Escherichia coli AN1460 | - |
- |
- |
2.7.1.25 | Saccharomyces cerevisiae | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.7.1.25 | ammonium sulfate, Blue Sepharose, Sephacryl S-200, PBE 94, Red Sepharose, phenyl-sepharose | Saccharomyces cerevisiae |
2.7.1.25 | ammonium sulfate, Giogel P2, Blue Sepharose, Sephacryl S-300, PBE 94 | Escherichia coli |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
2.7.1.25 | 0.614 | - |
- |
Escherichia coli |
2.7.1.25 | 0.614 | - |
- |
Saccharomyces cerevisiae |
2.7.1.25 | 0.843 | - |
- |
Escherichia coli |
2.7.1.25 | 0.843 | - |
- |
Saccharomyces cerevisiae |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.7.1.25 | ATP + adenosine 5-phosphosulfate | i.e. adenylylsulfate or APS | Escherichia coli | ADP + 3'-phosphoadenosine 5'-phosphosulfate | i.e. 3'-phosphoadenylylsulfate or PAPS, via a phosphorylated enzyme intermediate | ? | |
2.7.1.25 | ATP + adenosine 5-phosphosulfate | i.e. adenylylsulfate or APS | Saccharomyces cerevisiae | ADP + 3'-phosphoadenosine 5'-phosphosulfate | i.e. 3'-phosphoadenylylsulfate or PAPS, via a phosphorylated enzyme intermediate | ? | |
2.7.1.25 | ATP + adenosine 5-phosphosulfate | i.e. adenylylsulfate or APS | Escherichia coli AN1460 | ADP + 3'-phosphoadenosine 5'-phosphosulfate | i.e. 3'-phosphoadenylylsulfate or PAPS, via a phosphorylated enzyme intermediate | ? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
2.7.1.25 | dimer | 2 * 28000-29500, dithiothreitol-treated enzyme, SDS-PAGE | Saccharomyces cerevisiae |
2.7.1.25 | dimer | 2 * 40000-45000, dithiothreitol-treated enzyme, SDS-PAGE | Escherichia coli |
2.7.1.25 | tetramer | monomers and dimers are catalytically active | Escherichia coli |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.7.1.25 | additional information | - |
pI: 5.5 | Escherichia coli |
2.7.1.25 | additional information | - |
pI: 5.5 | Saccharomyces cerevisiae |
2.7.1.25 | additional information | - |
pI: 7.4 | Escherichia coli |
2.7.1.25 | additional information | - |
pI: 7.4 | Saccharomyces cerevisiae |
2.7.1.25 | 6.8 | 7 | - |
Escherichia coli |
2.7.1.25 | 6.8 | 7 | - |
Saccharomyces cerevisiae |
2.7.1.25 | 8 | - |
- |
Escherichia coli |
2.7.1.25 | 8 | - |
- |
Saccharomyces cerevisiae |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
2.7.1.25 | 6 | 8 | continuous increase of activity from pH 6.0 to 8.0 | Escherichia coli |
2.7.1.25 | 6 | 8 | continuous increase of activity from pH 6.0 to 8.0 | Saccharomyces cerevisiae |
2.7.1.25 | 6.8 | 8 | maximal activity at pH 6.8, approx. half-maximal activity at pH 8.0 | Escherichia coli |
2.7.1.25 | 6.8 | 8 | maximal activity at pH 6.8, approx. half-maximal activity at pH 8.0 | Saccharomyces cerevisiae |