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Literature summary extracted from

  • Yu, M.; Martin, R.L.; Jain, S.; Chen, L.J.; Segel, I.H.
    Rat liver ATP-sulfurylase: purification, kinetic characterization, and interaction with arsenate, selenate, phosphate, and other inorganic oxyanions (1989), Arch. Biochem. Biophys., 269, 156-174.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.7.4 ClO3- competitive with SO42- and apparently uncompetitive with respect to MgATP2- Rattus norvegicus
2.7.7.4 ClO4- competitive with SO42- and apparently uncompetitive with respect to MgATP2- Rattus norvegicus
2.7.7.4 diphosphate mixed-type inhibitor with respect to both MgATP2- and MoO42- Rattus norvegicus
2.7.7.4 FSO3- competitive with SO42- and apparently uncompetitive with respect to MgATP2- Rattus norvegicus
2.7.7.4 NO3- competitive with SO42- and apparently uncompetitive with respect to MgATP2- Rattus norvegicus
2.7.7.4 S2O32- noncompetitive mixed-type inhibition with respect to MgATP2- Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.7.4 0.012
-
 ATP 30°C, pH 8.0, reaction with CrO42- Rattus norvegicus
2.7.7.4 0.012
-
 ATP ATP in form of MgATP2- Rattus norvegicus
2.7.7.4 0.019
-
 ATP 30°C, pH 8.0, reaction with SeO42- Rattus norvegicus
2.7.7.4 0.019
-
 ATP ATP in form of MgATP2- Rattus norvegicus
2.7.7.4 0.1
-
 SeO42- 30°C, pH 8.0 Rattus norvegicus
2.7.7.4 0.12
-
 CrO42- 30°C, pH 8.0 Rattus norvegicus
2.7.7.4 0.13
-
 ATP 30°C, pH 8.0, reaction with MoO42- Rattus norvegicus
2.7.7.4 0.13
-
 ATP ATP in form of MgATP2- Rattus norvegicus
2.7.7.4 0.18
-
 SO42- 30°C, pH 8.0 Rattus norvegicus
2.7.7.4 0.21
-
 ATP 30°C, pH 8.0, reaction with SO42- Rattus norvegicus
2.7.7.4 0.21
-
 ATP ATP in form of MgATP2- Rattus norvegicus
2.7.7.4 0.24
-
 MoO42- 30°C, pH 8.0 Rattus norvegicus
2.7.7.4 0.27
-
 ATP 30°C, pH 8.0, reaction with WO42- Rattus norvegicus
2.7.7.4 0.27
-
 ATP ATP in form of MgATP2- Rattus norvegicus
2.7.7.4 0.47
-
 WO42- 30°C, pH 8.0 Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.7.4 cytosol
-
 Rattus norvegicus 5829
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.25 Mg2+ activation Rattus norvegicus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.7.4 62000
-
 2 * 62000, SDS-PAGE Rattus norvegicus
2.7.7.4 122000
-
 gel filtration Rattus norvegicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.7.4 ATP + sulfate Rattus norvegicus first enzyme of the two-step sulfate activation sequence diphosphate + adenylylsulfate
-
 r

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.25 Rattus norvegicus
-
-
-
2.7.7.4 Rattus norvegicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.7.4
-
 Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.1.25 liver
-
 Rattus norvegicus
-
2.7.7.4 liver
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.7.4 14.2
-
-
 Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.25 ATP + adenosine 5-phosphosulfate i.e. adenylylsulfate or APS Rattus norvegicus ADP + 3'-phosphoadenosine 5'-phosphosulfate i.e. 3'-phosphoadenylylsulfate or PAPS, via a phosphorylated enzyme intermediate r
2.7.7.4 ATP + chromate
-
 Rattus norvegicus diphosphate + adenylyl chromate
-
 r
2.7.7.4 ATP + CrO42-
-
 Rattus norvegicus AMP + adenylyl-chromate followed by nonenzymatic reaction of adenylylmolybdate with H2O to AMP and molybdate ?
2.7.7.4 ATP + molybdate
-
 Rattus norvegicus diphosphate + adenylyl molybdate
-
 r
2.7.7.4 ATP + MoO42-
-
 Rattus norvegicus AMP + adenylylmolybdate
-
 ?
2.7.7.4 ATP + selenate
-
 Rattus norvegicus diphosphate + adenylyl selenate
-
 r
2.7.7.4 ATP + SeO42- 20% of the activity with SO42- Rattus norvegicus AMP + adenylylselenate reaction is followed by nonenzymatic reaction of adenylylselenate with H2O to AMP and SeO42- ?
2.7.7.4 ATP + sulfate
-
 Rattus norvegicus diphosphate + adenylylsulfate
-
 r
2.7.7.4 ATP + sulfate first enzyme of the two-step sulfate activation sequence Rattus norvegicus diphosphate + adenylylsulfate
-
 r
2.7.7.4 ATP + sulfate
-
 Rattus norvegicus diphosphate + adenylyl sulfate
-
 r
2.7.7.4 ATP + tungstate
-
 Rattus norvegicus diphosphate + adenylyl tungstate
-
 r
2.7.7.4 ATP + WO42-
-
 Rattus norvegicus AMP + adenylyl-wolframate followed by nonenzymatic reaction of adenylyl-WO42- with H2O to AMP and WO42- ?

Subunits

EC Number Subunits Comment Organism
2.7.7.4 dimer 2 * 62000, SDS-PAGE Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.7.4 7 9 Tris-MES buffer Rattus norvegicus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.7.7.4 0.001
-
 diphosphate pH 8.0, 30°C, at 20 mM SO42- and 5 mM excess Mg2+ Rattus norvegicus
2.7.7.4 0.033
-
 ClO3- pH 8.0, 30°C Rattus norvegicus
2.7.7.4 0.28
-
 ClO4- pH 8.0, 30°C Rattus norvegicus
2.7.7.4 0.5
-
 FSO3- pH 8.0, 30°C Rattus norvegicus
2.7.7.4 1.13
-
 S2O32- pH 8.0, 30°C Rattus norvegicus
2.7.7.4 1.2
-
 NO3- pH 8.0, 30°C Rattus norvegicus