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Literature summary extracted from

  • Garavaglia, S.; Galizzi, A.; Rizzi, M.
    Allosteric regulation of Bacillus subtilis NAD kinase by quinolinic acid (2003), J. Bacteriol., 185, 4844-4850.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.1.23 quinolinic acid allosteric activator Bacillus subtilis

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.23 expression in Escherichia coli Bacillus subtilis

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.23 HgCl2 1 mM, 75% inhibition Bacillus subtilis
2.7.1.23 NADP+ 0.05 mM, 75% inhibition Bacillus subtilis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.23 1
-
 NAD+
-
 Bacillus subtilis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.23 30000
-
 2 * 30000, SDS-PAGE Bacillus subtilis
2.7.1.23 60000
-
 gel filtration Bacillus subtilis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.23 ATP + NAD+ Bacillus subtilis key enzyme for NADP+ metabolism and quinolinic acid metabolism ADP + NADP+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.23 Bacillus subtilis
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.23 ATP + NAD+
-
 Bacillus subtilis ADP + NADP+
-
 ?
2.7.1.23 ATP + NAD+ key enzyme for NADP+ metabolism and quinolinic acid metabolism Bacillus subtilis ADP + NADP+
-
 ?
2.7.1.23 dATP + NAD+ 77% of the activity with ATP Bacillus subtilis dADP + NADP+
-
 ?
2.7.1.23 dCTP + NAD+ 30% of the activity with ATP Bacillus subtilis dCDP + NADP+
-
 ?
2.7.1.23 dGTP + NAD+ 77% of the activity with ATP Bacillus subtilis dGDP + NADP+
-
 ?
2.7.1.23 diphosphate + NAD+ 10% of the activity with ATP Bacillus subtilis phosphate + NADP+
-
 ?
2.7.1.23 dTTP + NAD+ 35% of the activity with ATP Bacillus subtilis dTDP + NADP+
-
 ?
2.7.1.23 GTP + NAD+ 69% of the activity with ATP Bacillus subtilis GDP + NADP+
-
 ?
2.7.1.23 polyphosphate + NAD+ 50% of the activity with ATP Bacillus subtilis ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.1.23 dimer 2 * 30000, SDS-PAGE Bacillus subtilis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.1.23 35
-
-
 Bacillus subtilis

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.7.1.23 25 50 25°C: about 45% of maximal activity, 50°C: about 70% of maximal activity Bacillus subtilis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.7.1.23 40
-
 15 min, 50% loss of activity Bacillus subtilis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.7.1.23 1.36
-
 ATP
-
 Bacillus subtilis
2.7.1.23 5.46
-
 NAD+
-
 Bacillus subtilis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.23 9
-
 glycine-NaOH buffer Bacillus subtilis

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.7.1.23 7 10 pH 7.0: about 35% of maximal activity, pH 10.0: about 50% of maximal activity Bacillus subtilis

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.1.23 NAD+
-
 Bacillus subtilis