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Literature summary extracted from

  • Chung, A.E.
    Nicotinamide adenine dinucleotide kinase from Azotobacter vinelandii. I. Purification and properties of the enzyme (1967), J. Biol. Chem., 242, 1182-1186.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.23 2'-AMP 2 mM, 25% inhibition Azotobacter vinelandii
2.7.1.23 3'-AMP 2 mM, 20% inhibition Azotobacter vinelandii
2.7.1.23 3-acetylpyridine-adenine-dinucleotide (oxidized form) 0.5 mM, 10% inhibition Azotobacter vinelandii
2.7.1.23 5'-AMP 2 mM, 15% inhibition Azotobacter vinelandii
2.7.1.23 ADP 2 mM, 15% inhibition Azotobacter vinelandii
2.7.1.23 CH3HgBr 0.1 mM, 63% inhibition Azotobacter vinelandii
2.7.1.23 deaminopyridinealdehyde adenine dinucleotide 0.5 mM, 10% inhibition Azotobacter vinelandii
2.7.1.23 DTNB 0.1 mM, 74% inhibition Azotobacter vinelandii
2.7.1.23 Hg(CH3COO)2 0.1 mM, complete inhibition Azotobacter vinelandii
2.7.1.23 NADH 0.1 mM, 20% inhibition Azotobacter vinelandii
2.7.1.23 nicotinamide mononucleotide 2 mM, 15% inhibition Azotobacter vinelandii
2.7.1.23 PCMB 0.01 mM, 79% inhibition, NAD+ and ATP protect from inactivation Azotobacter vinelandii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.23 0.4
-
 NAD+ 37°C Azotobacter vinelandii
2.7.1.23 1
-
 ATP 37°C Azotobacter vinelandii

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.23 Mg2+ optimal concentration: 5 mM Azotobacter vinelandii

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.23 Azotobacter vinelandii
-
 ATCC 9104
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.23 0.233
-
-
 Azotobacter vinelandii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.23 ATP + NAD+
-
 Azotobacter vinelandii ADP + NADP+
-
 ?
2.7.1.23 CTP + NAD+
-
 Azotobacter vinelandii CDP + NADP+
-
 ?
2.7.1.23 GTP + NAD+
-
 Azotobacter vinelandii GDP + NADP+
-
 ?
2.7.1.23 ITP + NAD+
-
 Azotobacter vinelandii IDP + NADP+
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.7.1.23 additional information
-
 ATP, NAD+ and 2-mercaptoethanol stabilize against thermal inactivation Azotobacter vinelandii
2.7.1.23 20
-
 20 min, 12% loss of activity Azotobacter vinelandii
2.7.1.23 30
-
 20 min, 37% loss of activity Azotobacter vinelandii
2.7.1.23 38
-
 20 min, 70% loss of activity. 5 min, about 50% loss of activity Azotobacter vinelandii

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.1.23 NAD+
-
 Azotobacter vinelandii